NCI-H747[H747]细胞：人盲肠癌复苏细胞系更多折扣 货号：GD-C5920390
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
BisMuth subgallate hydrate
Bulleyaconitine A
2-(3-Benzoylphenyl)propionitrile
Asiatic acid
0
3-(TrifluoroMethyl)benzaldehyde
1,1′-Bis[(2R,5R)-2,5-diisopropylphospholano]ferrocene
2-(Methylthio)nicotinic acid, 97%
cis-2-Methylcyclohexanol
Dansylglycine
6-O-(Triisopropylsilyl)-D-galactal
(S)-(+)-4-Phenyl-2-oxazolidinone
1-METHYLTHIO-2-PROPANONE
Nafamostat mesylate
5-AMino-2-chloropyridine
3,6-Dichloropyridazine
1,3-Dihydro-1-Methoxyisobenzofuran
Methyl 2-broMo-4,5-difluorobenzoate
Xylitol, 99+%
Sorbitan Sesquioleate, NF
N-Acetyl-2,3-didehydro-2-deoxyneuraMinic Acid
tricosane
D-(-)-Gulonic acid gamma-lactone
3,5-diphenyl-1H-pyrazole
4-Fluorobenzamidine hydrochloride
POLYPHENYLMETHYLDIMETHYLSILOXANE
Guanosine-5'-diphosphate disodium salt
Ethyl ethoxyacetate
EthylenediaMinetetraacetic acid, iron(III) MonosodiuM salt
1,3-Diphenylisobenzofuran, 97%
1,2:5,6-Di-O-isopropylidene-α-D-allofuranose
ChloroMethyl chlorosulfate
ERYTHROSIN B
Oxindole-4-boronic acid, pinacol ester
2-BroMo-6-Methoxybenzaldehyde
4-AcetaMido-1-(3-chloro-2-pyridyl)piperidine, 96%
Poly(benzyl methacrylate), average M.W. 70000
Duroquinone, 97%
Potassium (4-methoxy)benzyloxymethyltrifluoroborate
Methyl 2-methyl-3-furancarboxylate, 97%
Ethyl 4-hydroxyphenylacetate
4-tert-Amylphenol
Ferric ammonium oxalate trihydrate
Hexyl acetate;n-Hexyl acetate
4-Methoxy-alpha-toluenethiol
5-CouMarancarboxaldehyde
4-Nitrotoluene-2-sulfonic Acid Hydrate
D-GlutaMic acid alpha-aMide, HCl
PalladiuM(II) nitrate hydrate
Cineole
1-EthylpyridiniuM Chloride
ethyl 4-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridine-5-car...
1-Amino-3,3-diethoxypropane
3-METHOXYPYRIDINE 1-OXIDE
barium diethyl bis(sulphate)
2-Bromo-4'-methoxyacetophenone
Ethylene glycol
Cytidine 5'-Monophosphate DisodiuM Salt Hydrate, 99%
2-FLUORO-6-METHOXYBENZOIC ACID
4'-Hydroxyhexanophenone
2,2-Difluorosuccinic acid
1,2-Dibromohexane, 97+%
3-Iodo-4-methoxy-1H-pyrrolo[2,3-b]pyridine
Methyl 2-benzoylbenzoate
5-Chloro-2-methoxyaniline
Fmoc-O-benzyl-L-tyrosine
2-Allyloxyethanol
4-MethyluMbelliferyl-beta-D-glucopyranoside
1-BroMo-3-(triMethylsilyl)benzene
4-(1,3-Dioxolan-2-yl)-2,6-difluorophenylboronic acid
D-Cysteine, 99%
{2-[1-(tert-Butoxycarbonyl)piperidin-4-yl]-5-Methyl-1,3-thiazol-4-yl}acetic acid, 97%
1,1'-SulfonyldiiMidazole
PentafluorobenzaMide
3-Acetylthianaphthene
2-Methoxy-4-(piperazin-1-yl)phenol
Boc-D-pyroglutaminol
2,3-Dihydroxypyridine
MPO-ANCA IgG ELISA Kit
Salusin a ELISA Kit
Human FBN1 (Fibrillin 1) ELISA Kit
Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) ELISA Kit
β-lactamase ELISA Kit
α-SYNo ELISA Kit
T4 ELISA Kit
CHE ELISA Kit
Human alpha-granular membrane protein,GMP-140 ELISA Kit
Amylin ELISA Kit
Halophilic Bacteria Selective Agar
Eugon LT 100 Agar Base
(4-Nitrophenyl)Methyl 3-oxobutanoate
AntiMony trifluoride
5,6-Diphenyl-2-Morpholinone, 98%
2-Fluoro-4-Methylpyridine-5-Boronic Acid
tricosane
2-pentenenitrile
Lanthanum(III) nitrate hexahydrate
Kojic acid
(1S,4R)-2-AZABICYCLO[2.2.1]HEPTAN-3-ONE
ETHIOFENCARB-SULFOXIDE
2,6-Dihydroxyacetophenone
5-Methyl-2-(3-nitrophenyl)-3-pyrazolinone, 98%
(S)-(-)-N-(3-Pentyl)-1-phenylethylaMine hydrochloride, 99%
Hexanophenone, 98%
2-Fluorotoluene
D-Leucinol
2-(N-Boc)-5-(tributylstannyl)thiazole
5-CHLORO-3-METHYL-1-PHENYL-1H-PYRAZOLE-4-CARBONITRILE
2-FLUORO-5-METHOXYBENZALDEHYDE
2-AMino-3-chloro-5-trifluoroMethylpyridine
Diethyl dimethylmalonate, 97%
n-Octanohydrazide
Phthalimidoacetone
