A-172[A172]细胞：人脑胶质瘤复苏细胞系更多折扣 货号：GD-C5920373
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-DiMethylaMinophenol, 97+%
3-AMino-2-Methoxypyridine
4-(3-Ethylureido)phenylboronic acid, pinacol ester
1-Isocyanato-5,6,7,8-tetrahydronaphthalene
POTASSIUM TETRACYANONICKELATE(II) HYDRATE
ethyl 2,4-dichloropyriMidine-5-carboxylate
2-Fluoro-4-nitrobenzoic acid
5-Hydroxyisoquinoline
1,2-Bis(dimethylphosphino)ethane, 98% DMPE
3,3'-Dibromo-4,4'-dimethoxybiphenyl
3,4,5-Trichloropyridazine
1-TERT-BUTOXYCARBONYLAMINO-CYCLOHEXANECARBOXYLIC ACID
N4-(2-METHYLPROPYL)-3,4-QUINOLINEDIAMINE
Benzoylferrocene
2,2-DiMethyl-2,3-dihydro-1-benzofuran-7-carbaldehyde
D(+)-Sucrose
DiMethyl 2,2'-Azobis(2-Methylpropionate)
Methyl 8-broMo-4-chloro-5-fluoroquinoline-2-carboxylate
Vinylbenzyl alcohol, 90%, Mixture of isoMers, stabilized
SodiuM Sulfate, Anhydrous, USP, EP, BP
2,6-DibroMoquinone-4-chloroiMide
2,5-Dimethylthiophene
Methylene dithiocyanate
3-Anilinopropionitrile
3-Fluorobenzoic acid
N-Boc-(2S,3S)-(-)-2-Amino-3-methyl-1-pentanol
5-Bromothiophenesulfonyl chloride
(S)-2-Methylpyrrolidine hydrochloride
endo-N-Hydroxy-5-norbornene-2,3-dicarboxiMide, 97%
6-FluorochroMan-4-one, 97%
Octafluoronaphthalene
1-BroMo-2,6-dichlorobenzene
3-Hexylthiophene
PENTAAMMINECHLOROCOBALT(III) CHLORIDE
3-CHLOROISONICOTINALDEHYDE
3,4-DICHLOROPHENYL ISOTHIOCYANATE
4-Fluorophenylboronic acid, May contain varying amounts of anhydride, 97%
N-(2-N-Boc-Amino-acetyl)-glycine
4-(tert-Butylcyclohexen-1-yl)boronic acid 97%
4-ethoxy-3-(trifluoromethyl)aniline(SALTDATA: HCl)
2,4-Dimethoxybenzylamine hydrochloride
Tetraethylene glycol dimethyl ether
5-METHYLINDOLE-3-ACETIC ACID
DI-N-BUTYL SULFOXIDE
1,1-Diphenyl-2-propyn-1-ol
2-AMinobenzotriazole
L-1,2,3,4-TetrahydronorharMan-3-carboxylic acid
4-AMino-1-benzylpiperidine dihydrochloride hydrate, 95%
4,5,6,7-Tetrahydropyrazolo[1,5-a]pyridine-3-carboxylic acid, 97%
4-Nitropyridine N-oxide
2-ChlorobenzylaMine
Pyrrolidine
trans-4-(3,4-Difluorophenyl)-trans-4'-vinylbicyclohexane
2-methyl-5-benzothiazolol
L-prolinamide hydrochloride
TRI-N-HEXYLAMINE
(DichloroMethylene)diMethylaMMoniuM chloride
6-CHLORO-2-FLUORO-3-METHYLBENZOYL CHLORIDE
2,3-BIS(HYDROXYMETHYL)NAPHTHALENE
2-Isopropyl-3-methoxypyrazine
Polymethyloctadecylsiloxane
5-Bromo-4-chloro-2-fluorotoluene 97%
1-(tert-butoxycarbonyl)-2-methyl-L-proline(SALTDATA: FREE)
Fmoc-L-Serine
THIONAZIN
3,5-DIMETHOXYPHENYL ISOTHIOCYANATE
TolazaMide
Stearic anhydride
1-BroMo-2-butanol (contains ca. 20% 2-BroMo-1-butanol)
4-MethoxypicolinaMide
6-Methoxypurine, 99+%
1H-Indazole-4-carboxaldehyde, 97%
3-Acetylthiazolidine-2-thione
2-Methyl-6-nitrobenzoyl Chloride
methazolamide
2,3,4-trifluorocinnamic acid
7-hydroxy-1H-indole-2-carboxylic acid
(phenylmethoxy)acetaldehyde
PLGF ELISA Kit
Smad1 ELISA Kit
Human PAEP (Progestagen-Associated Endometrial Protein) ELISA Kit
E1/UBAE ELISA Kit
IL-10 ELISA Kit
Rat α-CTx (Alpha Crosslaps) ELISA Kit
MMP13 ELISA Kit
Human glial fibrillary acidic protein,GFAP ELISA Kit
Human HPX ELISA Kit
IRS-2 ELISA Kit
Soybean-Casein Digest Agar Medium with Lecithin
Bromcresol Purple Peptone Broth
Acetazolamide
YtterbiuM(III) fluoride, anhydrous, 99.9% (REO)
5,6-Dihydro-5-Methyluracil, 98+%
2-Fluoro-4-nitrobenzotrifluoride, 98%
2,5-Dimethylthiophene
2,6-dimethylheptan-2-ol
5-BROMO-1-BENZOFURAN-2-CARBOXYLIC ACID
carvacrol
1,3-Dibromo-2,2-dimethoxypropane
(Z)-9-Octadecenoic acid methyl ester
1H-Pyrrolo[2,3-C]Pyridine-2-Carboxylic Acid Ethyl Ester
2,3'-DibroMoacetophenone, 97%
2-(BroMoacetyl)naphthalene, 98%
CalciuM hydride
Resorcinol solution
4-(E-3-Ethoxy-3-oxo-1-propen-1-yl)phenylboronic acid
3-(4-FLUOROPHENYL)BENZALDEHYDE
12-AMinolauric Acid , 98%
1-Methyl-2-piperidineMethanol
8-Hydroxy-1,1,7,7-tetramethyljulolidine-9-carboxaldehyde, 99%
5-Cyano-4-methyl-1,2,3-thiadiazole ,97%
2-Bromo-1,3-dimethoxybenzene
2-Methoxy-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenylacetate
