U251 MG细胞：人星形胶质瘤复苏细胞系更多折扣

U251 MG细胞：人星形胶质瘤复苏细胞系更多折扣 货号：GD-C5920588
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
1,3-Dibenzoyloxybenzene, 98%
NaphtholAS
2,5-Norbornadiene
2-Nitrophenylacetic acid
ACRYLIC ACID ISOAMYL ESTER
2-AMinobenziMidazole
L-MANNITOL
Sodium tri-sec-butylborohydride, 1M solution in tetrahydrofuran
Ethyl 3,4,5-trimethoxybenzoylacetate
N-Ethyl-N-sulphopropyl-m-toluidine, sodium salt >98%
BOC-D-Alanine methyl ester, 99%
(2-AMINOETHOXY)DIPHENYLBORANE
3,4-Diaminobenzoic acid
Lithium chromate
4-Methyl-3-(trifluoromethyl)aniline
2,5-Dihydrofuran
(S)-(-)-N,N-DiMethyl-1-ferrocenylethylaMine
4-BroMo-1-(pyridin-4-ylMethyl)pyrazole
Sudan Red 7B
Mesterolone (CIII)
N-Ethylglycine
3,3'-Dihydroxy-beta,beta-carotene-4,4'-dione
Tris(trimethylsiloxy)boron
1,6-Diiodohexane
2-Heptanone
4-Biphenylboronic acid
2-Fluoro-5-bromobenzotrifluoride
1-Methyl-Piperidine-3-Carboxylic Acid Methyl Ester
3-(MethylsulfonylaMino)benzeneboronic acid, 97%
4-Hydroxy-3,5-diMethoxybenzyl alcohol, 97%
1-Nonanethiol
DiaMMine dichloropalladiuM (Ⅱ)
TRIS(2-AMINOETHYL)AMINE
TEREPHTHALIC DIHYDRAZIDE
CHLORANILIC ACID
(2-Chloro-3-pyridinyl)Methanol
Methylthiomethyl p-Tolyl Sulfone
3-Amino-3-(4-fluorophenyl)propanoic acid
3,5-Dimethylphenylhydrazine hydrochloride 98%
2-Amino-6-bromonaphthalene
6-CHLORO-2,4-DIMETHOXYPYRIMIDINE
(4-CHLORO-PHENYL)-METHANESULFONYL CHLORIDE
Methyl 4-amino-3-iodobenzoate
AZOCARMINE B
2-(Dicyclohexylphosphino)-2'-Methylbiphenyl
Carbohydrazide
4-Methyl-4-cyclohexene-1,2-dicarboxylic Anhydride
2-AcetaMido-5-fluoropyridine
Manganese(II) broMide, 99%, anhydrous
BOC-L-BETA-HOMOLEUCINE
(S)-(+)-CaMptothecin
3,5-Dibromo-2-hydroxypyridine
N-methyl-3-(trimethoxysilyl)-N-[3-(trimethoxysilyl)propyl]propan-1-amine
5-METHOXY-PYRIDIN-2-YLAMINE
4-Nitrobenzyl chloroformate
Phenol Red sodium salt
5,6-DICHLOROBENZIMIDAZOLE-2-THIOL
2,3-Pyrazinedicarboxylic acid
BIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)COPPER(II)

6-Methyl-2-p-tolyl-imidazo[1,2-a]pyridine
Diphenyl difluorosilanegas cylinder

Dimethyl malate
D-Phenylalanine methyl ester hydrochloride
METHYL 2-CYANOBENZOATE
4-Methoxystyrene
3-(TrifluoroMethyl)styrene
1-(4-Carboxyphenyl)-5-Mercapto-1H-tetrazole
1-(Toluene-4-sulfonyl)-1H-indole-3-boronic acid pinacol ester
IsophorondiaMine, 99+%, Mixture of cis and trans
(S)-(-)-Nalpha-DiMethylbenzylaMine, 99+%

4-BroMo-1,2-Methylenedioxybenzene
7-fluoro-3,4-dihydronaphthalen-1(2H)-one
3-Chlorophenol
2-amino-2-(4-bromophenyl)ethanol
Amidosulfuron
PRDX2 ELISA Kit
GSTa2 ELISA Kit
Human TNFα ELISA Kit
Human MRC2 ELISA Kit
HSP90β1 ELISA Kit
Rat cluster Of differentiation,CD44 ELISA Kit
IL-6 ELISA Kit
Human BEST2 ELISA Kit
Human heart fatty acid binding protein,h-FABP ELISA Kit
GDF10 ELISA Kit
BPLS Agar
PYG Broth Medium Base
CORN OIL
Pectin FroM Citrus
5-Decanone, 99%
2-MethyliMidazole
3,3'-Dihydroxy-beta,beta-carotene-4,4'-dione
Iron(III) nitrate nonahydrate
1-Benzocyclobutenecarbonitrile
Dicyclohexyl ketone

1H-Pyrazole-1-carboxamidine hydrochloride
DINOBUTON
3-AMino-4-Ethoxypyridine
Tris(isopropylcyclopentadienyl)erbiuM(III), 99.9% (REO)
12-Chloro-5-dodecyne, 98%
His-Gly
Ethyl 5-hydroxy-2-Methylindole-3-carboxylate
4-Nitrobenzenesulfenyl chloride
1-Methyl-4-(trifluoroMethylsulfonyl)benzene
4-MethylaMino-3-nitrophenylboronic acid, pinacol ester
1,5-Naphthalenedisulfonic acid disodiuM salt hydrate
Methyl 3-formylindole-6-carboxylate
Gallic acid ,99%
Ethyl 4-(trifluoromethyl)benzoate , 97%
6-Chloro-N-isopropyl-2-pyrazinamine