SJRH30细胞：人横纹肌肉癌复苏细胞系更多折扣

SJRH30细胞：人横纹肌肉癌复苏细胞系更多折扣 货号：GD-C5920560
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-EthoxybenzaMide, 97%
N-Cbz-4-aMinocyclohexanone
2-(TrifluoroMethoxy)phenylacetic acid
3-NITROBENZENESULFONAMIDE
1-(6-BroMopyridin-2-yl)piperidin-4-ol
1-BroMo-2-iodo-4-(trifluoroMethyl)benzene
Ethyl trans-2-decenoate, 97%
2-(Adamant-1-yl)propan-2-ol
Lauric Acid
2,2,6,6-Tetramethyl-3,5-heptanedionato lithium, 98+% [Li(TMHD)]
1,1-Diphenyl-2-propanol, 98%
(+/-)-HYDROBENZOIN
2,4-Dichloro-6-phenyl-1,3,5-triazine
Bromothymol Blue
4-Bromomethylbenzoic acid
2,6-Difluorobenzophenone
N,N-DiMethyl-3,5-dinitroaniline
4-BroMo-1H-pyrazole-3-carboxaMide
Stachyose hydrate, 97+%
M-Cresol Purple
CarbaMazepin
hydromorphone
benzyl B-D-glucopyranoside
Pyridine Hydrochloride
Tetrahydrofuran
4-ETHYL-3-HEXANOL
2,4-DICHLOROANILINE
3-Methylbutanal
3-(4-Fluorophenyl)-1H-pyrazole, 97%
1-(2,4,6-Triisopropylphenylsulfonyl)-1,2,4-triazole, 98%
L-Valine-15N
2,4-Dihydroxybenzaldehyde
2-Cyclopentylethanol, 98+%
tert-ButylaMine
CHROMIUM (III) CHLORIDE
HYDANTOIC ACID
Ethylamine Hydrobromide
5-Aminosalicylic acid, purified, 99%
N,N-Dimethyl-m-phenylenediamine ,99%
5-Methyl-2-pyrrolidone
Thianaphthene
Rhodanine-3-acetic acid
2,3-Dichlorothiophene-5-sulphonyl chloride
Calcium nitrate tetrahydrate
Genistein
3,5-DiMethylphenylhydrazine hydrochloride
MethylMaleic Hydrazide
(3-Fluoropyridin-4-yl)MethanaMine
Triethyl 2-fluoro-2-phosphonoacetate, 96%
Biphenyl
trans-4-AcetaMidocyclohexanol
Methyl 1-cyclohexene-1-carboxylate
Ginsenoside Rg3
cyclohexyldiphenylphosphine oxide
S-Methyl thiopropionate
Diglycolic acid
1-Fluoro-3-iodobenzene
Methylenecyclobutane
1-Acetylpyrrole-3-boronic acid, pinacol ester
Tri-p-cresyl Phosphate
Methyl 2-chloro-4-nitrobenzoate
cis-1,2-Bis(phenylsulfonyl)ethylene
4,5-Dimethylisoxazole-3-carboxylic acid
PROPYLCYCLOHEXANE
1-(2,5-DIMETHOXYPHENYL)-2-THIOUREA
1-(4-CHLORO-3-TRIFLUOROMETHYLPHENYL)
5-Fluoroisatin
2-Propoxybenzoic Acid
1-(2-Cyanoethyl)-2-Methylpiperidine
3-(3-Morpholinopropyl)phenylboronic acid, pinacol ester, HCl
Glycerol forMal, 99+%, Mixture of isoMers, stabilized
RubidiuM fluoride
Linalyl Acetate
4-Phenylethynylphthalic Anhydride
4-Hydroxyindole
6-Nitrobenzimidazole
[2R-[2aR*),3a]-2-[1-[3,5-Bis(trifluoromethyl)phenyl]ethoxy]-3-(4-fluorophenyl)morpholine
5-O-Allyl-2,3,4-tri-O-benzyl-D-ribitol
INS ELISA Kit
IL25 ELISA Kit
Human TNFSF12 ELISA Kit
Human CLL1 ELISA Kit
LH ELISA Kit
CD163 ELISA Kit
Monkey α2-M (Alpha-2 Macroglobulin) ELISA Kit
Human C1qR1 (Complement Component 1q Receptor) ELISA Kit
Human cardiotrophln 1,CT-1 ELISA Kit
NGF ELISA Kit
Violet Red Bile Glucose Agar
Cysteine Heart Blood Agar with antibiotics
SodiuM orthovanadate, 99.9% (Metals basis)
tert-Butyl peroxybenzoate, 98%
5-Methyl-1H-indazole-3-carboxylic acid, 97%
2-METHYLUNDECANAL
hydromorphone
Potassium dichromate
4-phenyl-1H-Imidazol-2-amine
5-chloro-2-nitrobenzotrifluoride

(R)-Glycidyl butyrate
4-Bromobenzonitrile
MagnesiuM sulfate heptahydrate, 99+%
4-BroMosalicylaldehyde, 97%
EthyldiMethylchlorosilane, 97%
Ethyl trans-2-hexenoate
4-Chloro-3-fluorophenol
Cupric sulfate
4-AMino-2-broMopyridine
4-Ethylphenylacetylene
5-BroMo-2-piperazinopyriMidine
3-Maleimidopropionic acid ,98%
(R,R)-(+)-2,2'-Bis[(S)-(N,N-dimethylamino)(phenyl)methyl]-1,1'-bis(di(3,5-dimethylphenyl)phosphino)ferrocene, min. 97%
3-Methylbenzyl 1H-1,2,4-triazol-3-yl sulfide
5,7-Dichloro-3-ethylpyrazolo[1,5-a]pyrimidine