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Beta-TC-6细胞:小鼠胰岛素瘤胰岛β复苏细胞系更多折扣

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  • Beta-TC-6细胞:小鼠胰岛素瘤胰岛β复苏细胞系更多折扣 货号:GD-C5918691

    细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
    产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
    产品规格:≥1*10(6)/ml
    安全级别:1
    物种来源:人源或鼠源等
    细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
    细胞常规培养传代流程(请严格遵照无菌操作):1)吸出原培养瓶中的培养基,PBS缓冲液润洗细胞两次,加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间,通常控制在1-2min);2)镜下观察消化情况,在细胞边缘缩小,贴壁松动时(不建议消化到细胞漂浮)去掉胰酶,加6-8ml完全培养基,轻轻吹打细胞层,尽量把细胞层吹落,吹散;3)取部分细胞悬液转移到新的培养皿/瓶中,添加适当的完全培养基,把细胞悬液打匀,于培养箱中培养;4)注意培养基PH值变化情况,定期换液(每周2-3次),待细胞密度达到80%以后重复1项操作或者冻存;特别注意:(如使用公共实验室或者初次接触细胞培养,建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基,如因特殊情况需要继续使用原瓶,请在原瓶培养基中额外添加10%的血清,(原瓶培养基的继续使用时间Z长不宜超过72小时);2)贴壁细胞收到当天切忌立刻消化,请将细胞换液后放置培养箱孵育到第二天再做消化传代,请优先选择直径6cm的培养皿进行传代培养;3)如签收时出现培养瓶壁破裂,漏液等情况请及时做好照片记录并联系实验室。
    细胞培养实验中常见问题总结:1)一般客户拿到细胞后,应该注意什么?客户收到细胞后先不开盖,放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况,并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况,显微镜下拍细胞100X,200X各一张),排除细胞本身污染的情况;收到细胞未开封,出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况,请在显微镜下观察细胞密度,如为贴壁细胞,未超过80%汇合度时,将培养瓶中培养液吸出,留下10ml培养液继续培养;超过80%汇合度时,请按细胞培养条件传代培养。如为悬浮细胞,吸出培养液、1000转/分钟离心2分钟,吸出上清,管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制,慎用Hanks液配制;2)快递细胞多久能到,是寄冻存的细胞还是复苏好的细胞?我们采用快递发货,一般外地2--3天,寄细胞前请确认当地温度,如果气温低于4度的,则采用邮寄冻存细胞;3)可否使用与原先培养条件不同之培养基?不能。每一细胞株均有其特定使用且已适应之细胞培养基,若骤然使用和原先提供之培养条件不同之培养基,细胞大都无法立即适应,造成细胞无法存活;4)可否使用与原先培养条件不同之血清种类?不能。血清是细胞培养上一个极为重要的营养来源,所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清,在一些物质或分子的量或内容物上都有所不同,血清使用错误常会造成细胞无法存活。
    细胞特性:贴壁/悬浮等
    细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
    培养条件:详见细胞说明书
    传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
    2-(4-Hydroxyphenyl)propionic acid, 98%
    4-BroMothioanisole
    Trichlorovinylsilane
    TRIS[N,N-BIS(TRIMETHYLSILYL)AMIDE]CERIUM(III)
    1,3,5(10)-Estratrien-3-ol-17-one
    4-(4-Acetylpiperazino)phenylboronic acid, pinacol ester
    DL-ALANYL-GLYCINE
    Octatriacontane
    2-Fluoropyridine-3-carbonyl chloride ,98%
    3-Chloro-6-methoxy-benzo[b]thiophene-2-carboxylicacid
    4-(Thiomorpholin-4-ylcarbonyl)benzeneboronic acid 97%
    1-Phenylimidazole
    3,5-Dimethylbenzonitrile
    4-Fluorophenol
    5-Chlorovaleronitrile
    2-(4-Pyridyl)-1,3-thiazole-4-carboxylic acid
    1,3-DibroMoadaMantane
    6-BroMo-1h-pyrazolo[4,3-b]pyridine
    Staurosporine, 98%
    MefenaMic Acid, BP
    Stearyl Gallate
    3,5-dihydroxy-2,6,6-tris(3-methylbut-2-enyl)-4-(3-methyl-1-o...
    (S)-2-Amino-3-(4-ethylphenyl)propanoic acid
    butyl methyl sulphide
    2,5-Dimethyl-2,5-hexanediol
    2,4,6,8-TETRAMETHYLCYCLOTETRASILOXANE
    Sodium rhodizonate
    4-Iodoacetophenone
    5-BroMonicotinic acid, 98%
    1,5-Cyclooctadiene(pyridine)(tricyclohexylphosphine)iridiuM(I) hexafluorophosphate, 99%
    4-(Boc-aMino)-3-iodopyridine
    D-Panose
    5,6-DIACETOXYINDOLE
    TERT-BUTYL N-(TERT-BUTOXYCARBONYLOXY)CARBAMATE
    DI-U-CHLOROBIS[5-CHLORO-2-((4-CHLOROPHE&
    3-Fluoro-4-(4-Morpholinyl)aniline
    3,5-Dimethylphenol ,99%
    5-Methoxy-1H-pyrrolo[2,3-b]pyridine
    3-Aminoethyl-1-N-Cbz-pyrrolidine
    4-Amino-5-chloro-2,1,3-benzothiadiazole
    4-(Methylthio)phenylboronic acid
    BOC-(R)-3-AMINO-3-(4-FLUORO-PHENYL)-PROPIONIC ACID
    Methyl L-tyrosinate hydrochloride
    TRIMETHYLSULFONIUM HYDROXIDE
    6-BroMoquinoline
    Alizarin Astrol
    Ethyl 2-Methoxyisonicotinate
    3-BroMo-N-Methylaniline, 98%
    3-CarbaMoyl-2,2,5,5-tetraMethyl-3-pyrrolin-1-yloxy, 99%, free radical
    Boc-3-(2-pyridyl)-L-alanine
    Gestodene
    5-Chloro-2-phenoxyaniline
    4-trans-Propylcyclohexyl-4'-propylbiphenyl
    Butanamide,2,2'-[[3,3'-dichloro(1,1'-biphenyl)-4,4'diyl]bis(azo)]bis[3-oxo-N,N'-bis(p-anisyl and phenyl)derives]
    b-amino-3-fluoro-Benzenepropanol
    (S)-N-FMOC-4-Chlorophenylalanine
    DiallylcarbaMyl chloride
    5-BroMo-2-oxindole
    TRIETHYLSILANOL
    1-ETHYL-4-METHYLCYCLOHEXANE
    2-Ethylnaphthalene
    10-Undecenyltrichlorosilane
    Monofluorophosphoric acid, 70% in water
    5-[2-(Methylthio)ethyl]hydantoin
    Diethyl 5-nitroisophthalate
    N-Boc-2-pyrroleboronic acid
    3-Chloro-2,4,5-trifluorobenzoic acid
    Dimethyl 1,3-acetonedicarboxylate
    1-(2-Hydroxyethyl)pyrrolidine
    4,4'-Difluorobenzhydrol
    4-BroMo-1-Methyl-1H-iMidazole
    PotassiuM tetrachloroplatinate(II), 46-47% Pt
    Glycyl-L-tyrosine
    3,4-Difluorobenzophenone
    naphtho(2,3-A)pyrene
    Ac-Ile-OH
    norfenefrine
    Vitamin A Palmitate
    INS ELISA Kit
    Mouse Interleukin 23,IL-23 ELISA Kit
    Human TNFsR-Ⅱ ELISA Kit
    Human primary hepatic carcinoma,PHC ELISA Kit
    IFNβ ELISA Kit
    CD2 ELISA Kit
    BMG/β2-MG ELISA Kit
    Human asymmetrical dimethylarginine,ADMA ELISA Kit
    Human CLS (Cardiolipin Synthase) ELISA Kit
    Py ELISA Kit
    Fluid Tetrathionate Medium Base
    Meat Infusion Broth
    Triethyl Orthobutyrate
    BariuM Peroxide
    5-Methyl-1H-Pyrazole-3-Carboxylic Acid
    2-Methyl-1,3-Thiazole-5-Carboxylic Acid
    3,5-dihydroxy-2,6,6-tris(3-methylbut-2-enyl)-4-(3-methyl-1-o...
    Potassium Phosphate Monobasic
    N-Benzyl-2,4-Imidazolinedione
    2'-Hydroxyacetophenone

    TRIS(CYCLOPENTADIENYL)NEODYMIUM
    2-Pentylpyridine
    N-(4-Ethoxyphenyl)MaleaMic acid, 97%
    2,4,4,6-TetraMethyl-1-oxa-3-aza-2-cyclohexene, 95%
    2-Methylcyclopentane-1,3-dione, 98%
    Z-Aib-OH
    EDTA TETRASODIUM SALT: HYDRATE
    6-Fluoro-1H-indazole
    PROPYLMAGNESIUM CHLORIDE
    trans-3-Chloro-β-nitrostyrene
    ALPHA-BROMO-4-CHLOROPHENYLACETIC ACID
    2-Chloro-6-(trifluoromethyl)nicotinonitrile
    3-Methyl-1 H -pyrazole-4-carboxylic acid
    4-Bromo-2-difluoromethyl-1-fluorobenzene
    2-Propyl-4-(4-pyridylazo)phenol
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