Hs 578T细胞：人乳腺癌复苏细胞系更多折扣 货号：GD-C5919114
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Thiophene-2-carboxaldehyde, 98+%
Allyl alcohol
2,6-Bis[(2R,4S,5S)-1-benzyl-4,5-diphenyliMidazolidin-2-yl]pyridine
PentabroMoethane , 98%
BenzyltriphenylphosphoniuM broMide
L(-)-CaMphorsulfonic acid
(4-VINYLBENZYL) AMINE
N,N-Diethylacetamide, 99%
2'-Trifluoromethylbiphenyl-3-carboxylic acid
Nitranilic Acid Hexahydrate
Calcein-AM
4-Methyl-D-phenylalanine
4-(N,N-Diphenylamino)benzaldehyde
N-Ethyl-o-toluenesulfonamide
Hesperidin
4-(TrifluoroMethoxy)aniline hydrochloride
2,5-Dibenzylidenecyclopentanone
6-Undecanol
TriphenylMethyl Mercaptan, 98%
Ropivacaine Hydrochloride, USP
3,4-DiMethylheptane
N,N'-Diphenylformamidine
4'-fluorobiphenyl-4-carboxylate
9-aminophenanthrene
Methyl 5-fluoro-2-hydroxybenzoate
4-(4-N-PROPYLPHENYL)BENZOIC ACID
4-(BroMoacetyl)pyridine hydrobroMide
3-Benzoylthiophene-2-carboxylic acid, 96%
2,4,6-TriMethyl-1,6-heptadien-4-ol, 98%
Anise seed oil
Azetidine
Phloroglucinol
3′-AcetylaMino-4′-Methoxyacetophenone
1,3,5-Tri-tert-butylbenzene
3-Bromo-2-chloro-5-methylpyridine 97%
Propionic anhydride ,99%
1-Bromo-2-ethylbenzene
Dicyclohexylphosphinyl Chloride
Cyclopentadecanone
3-CHLOROBIPHENYL
4-AMINO-3-CHLORO-5-METHYLBENZONITRILE
4-Fluoroacetophenone
(R)-(+)-1-AMino-2-(MethoxyMethyl)pyrrolidine
CyclohexylMagnesiuM BroMide (ca. 18% in Tetrahydrofuran, ca. 1Mol/L)
2-PiperidinopyriMidine-5-boronic acid pinacol ester
Hexa-n-butylditin, 98%
Tetrahydro-3-furoic acid, 97%
1,5-Dichloro-2,4-dinitrobenzene
2-Chlorohexane
4-Chloro-3-fluoropyridine
Tetrahydropyran-2-methanol
fmoc-3-(3-benzothienyl)-D-alanine
L-glutamic-3-13C acid
2-Naphthalenethiol
2-Fluoro-3-nitrophenylboronic acid, pinacol ester
(R)-(?)-Pyrrolidine-3-carboxylic acid
(R)-3-AMino-1-carbobenzoxypyrrolidine Hydrochloride , 98%
Methoxyacetaldehyde dimethyl acetal
Methyl 4-(1H-1,2,4-triazol-1-yl)benzenecarboxylate
Acid Red 88
Benzeneseleninic anhydride, 98%
N,N'-Dimethyl-1,2-ethanediamine
2-ETHYLHEXYL ACRYLATE
D-Valine
Oxazole
Cyclobutanecarboxylic acid chloride
1-Acetylindoline
Ibudilast
6-Fluoro-8-Methylquinoline
Diphenyl(pentafluorophenyl)phosphine, 98%
(+/-)-Naringenin
VariaMine Blue B DiazoniuM Salt [for Naphthol-Dyes]
4-Bromo-3-chlorotoluene
ethyl 2-methylcyclopropanecarboxylate
(+/-)-Pseudopelletierine oxime
2,3-Difluorobenzaldehyde
MAU ELISA Kit
IFN-α1 ELISA Kit
Human NEO1 ELISA Kit
Human PSMD10 ELISA Kit
C4 ELISA Kit
TLR6 ELISA Kit
O2 ELISA Kit
Human colon cancer-specific antigen-4,CCSA-4 ELISA Kit
Human Angiopoietin 1,ANG-1 ELISA Kit
AGF ELISA Kit
Nielsen Medium
Anaerobic Agar
IsopropylMagnesiuM broMide, 3M in 2-MeTHF
GLYCOCHOLIC ACID SODIUM SALT
5-Chloro-2-fluorobenzaMide, 97+%
2'-Fluoro-6'-hydroxyacetophenone, 98%
N,N'-Diphenylformamidine
Solvent Black 7
Silk, powd.
4-bromo-4'-fluorobiphenyl
1,2,3,4-TETRAHYDROISOQUINOLINE HYDROCHLORIDE
4-FLUORO-1-NAPHTHOIC ACID
4-IodobenzylaMine Hydrochloride
Cobalt iron oxide, May contain sMall aMounts of iron oxide
Methyl 2,2-diMethylacetoacetate, 99%
AMberlite XAD-1180
4-Fluoro-2-(trifluoroMethyl)phenol
2-CYANO-4-METHYLPYRIDINE
3-Pentanol
4,5-Difluoro-2-isopropoxyphenylboronic acid
5-BroMo-3-pyridinecarboxaldehyde
2-Nitrophenol SodiuM Salt
5-Bromoindole-2-carboxylic acid
1-Ethyl-3-methylpyridinium Ethyl Sulfate
Hexadecyl β-D-maltopyranoside ,98% ,98%
