A549细胞：人肺癌复苏细胞系更多低价 货号：GD-C5819082
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Tri-n-butyl(1-ethoxyvinyl)tin
1-Chlorotetradecane
5-BroMo-2-(4-Methoxybenzyloxy)pyridine
Methyl 2-aMino-4-cyclopropylthiazole-5-carboxylate
N-HEXYLLITHIUM
METHYL 3-OXOHEPTANOATE
1-Eth-1-ynyl-4-(pentyloxy)benzene
(2S,4S)-(+)-2,4-Pentanediol, 99%
1-Methyl-2-imidazolidinone ,98%
Ethyl 3-ethyl-5-methylisoxazole-4-carboxylate
Butyl Benzenesulfonate
2-(Trifluoromethyl)benzene-1,4-diamine
2,4-DIAMINOANISOLE DIHYDROCHLORIDE
Nizatidine
3,5-Dichlorosalicylaldehyde
Guanidine carbonate
3,5-DichlorobenzenesulfonaMide
N-Isopropyl 4-broMobenzenesulfonaMide
TriMethylsilyl isothiocyanate, 97%
P-TOLUIDINE
4-BenzaMidopiperidine Hydrate
2,4-Dichloronitrobenzene
1,5-Diazocine,octahydro-
1,2,3,4-Tetrahydrocarbazole
Betamethasone
1-ACETOXYACENAPHTHENE
1-Bromo-2,4-difluorobenzene
2-BroMo-6-Methylpyridine
9-BBN diMer, 98%
trans-4-Decenal, 96%, May cont. ca 7% cis-isoMer
N-Acetyl-Val-Ala-Asp-al
(S)-(+)-1-Cbz-3-aMinopyrrolidine
6,8-Dichloro-3-MethylchroMone
Poly(diMethylsiloxane)
2,4-DiMethylbenzophenone
DYSPROSIUM CHLORIDE
Itaconic acid, 99+%
Rubidium iodide, 99%
1-(1H-indol-3-yl)ethanamine(SALTDATA: FREE)
(5-fluoro-1H-benzimidazol-2-yl)methanol(SALTDATA: FREE)
3-Ethyl-2-methyl-5-(morpholin-4-ylmethyl)-1,5,6,7-tetrahydroindol-4-one
9-Bromoanthracene
NEODYMIUM IODIDE
CIS-2-PENTEN-1-OL
1-(2-Methoxyphenyl) hydrochloride
Atrolactic Acid Hydrate
3-Pyrazol-1-yl-benzylaMine
3-Iodo-4-nitroanisole, 97%
6-(Tetrahydropyran-4-yloxy)pyridine-2-carboxylic acid, 97%
N-Acetyl-3,5-dinitro-L-tyrosine
N-Hydroxy-4-nitrophthaliMide
n-Propyl propionate
(S)-(-)-1-Benzyl-3-(Boc-Amino)Pyrrolidine
2-Bromo-5-methoxyphenol
3,4-Diaminobenzophenone
11-HEXADECYN-1-OL
Vinylbenzyl chloride
N-Hexyl 3-broMo-5-trifluoroMethylbenzenesulfonaMide
Z-L-ProlinaMide
6-Chloropyridine-3-carbohydrazide , 97%
Triphenylarsine oxide
N-(4-amino-2-methoxyphenyl)acetamide hydrochloride(SALTDATA: FREE)
1-Benzyl-4-(N-Boc-amino)piperidine
alpha-Cyclopentylmandelic acid
2-Amino-1-cyclopentene-1-carbonitrile
4-Bromoquinoline
Capecitabine Related CoMpound B;5'-deoxy-5-fluorouridine
2-Fluorobenzotrifluoride
11-Azido-3,6,9-trioxaundecanoic Acid
3-AMino-4-Methoxyphenylboronic acid hcl
4-Fluorobenzaldehyde, 98+%
4-(2-Oxo-1-pyrrolidinyl)benzenesulfonyl chloride, 97%
LAUREL LEAF OIL
trans-Stilbene
2,2-dichloro-1,1,1-trifluoroethane
1H-Pyrrole-2-carboxamide,1-amino-(9CI)
2-Amino-4-chloro-6-isopropylpyrimidine
4-propylcyclohexan-1-ol
GIP ELISA Kit
Mouse IDUA (α-L-iduronidase) ELISA Kit
Human EGR4 (Early Growth Response Protein 4) ELISA Kit
Human tumor marker DR-70 for lung cancer,DR-70TM ELISA Kit
HSVⅡ-Ab ELISA Kit
TARDBP ELISA Kit
Chicken Leukemia inhibitory factor,LIF ELISA Kit
SCTR ELISA Kit
VEGF.D ELISA Kit
AGTR1 ELISA Kit
Medium 2（Penassay Base Agar）
Clostridia Count Agar
Isobutylboronic acid, 97%
GLYCYL-D-VALINE
5-Chloro-2-Cyanopyridine
2-Fluoro-N-(3-fluorobenzyl)aniline, 97%
2,4-Dichloronitrobenzene
Fluorescent brightener 185
dipyrone
Benzoic acid,5-fluoro-2-nitro-, methyl ester
1-(4-Methoxyphenyl) hydrochloride
BREDERECK'S REAGENT
Diisopropyl Ether
2,3-Dichloro-1,4-naphthoquinone, 98%
2-AMino-N-(4-Methoxyphenyl)pyridine, 97%
3-Chloropropiophenone, 96%
Leptophos
1-METHOXY-4-NITRONAPHTHALENE
1-BOC-4-(4-nitrophenoxy)piperidine
Benzenesulfonic acid sodiuM salt
2,4,6-TRIMETHYLBENZENESULFONYL HYDRAZIDE
2-Ethyl-4,5-diMethylthiazole , 98%
2-(1-adamantyl)ethanamine(SALTDATA: FREE)
1H-Pyrrolo[2,3-b]pyridine-5-carboxylic acid
Diethyl Sebacate
