PC-3M-2B4细胞：人前列腺癌低转移复苏细胞系更多低价 货号：GD-C5819054
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2,4-Dinitroaniline, 99% (dry wt.), cont. up to 15% water
Diethyl (2-oxopropyl)phosphonate
1,3,5-Triacryloylhexahydro-1,3,5-triazine , 98%
1-N-BUTYL-1-PHENYLHYDRAZINE
ZINC CYCLOHEXANEBUTYRATE
2-METHYL-2-MORPHOLIN-4-YL-PROPYLAMINE
5-MORPHOLINO-2-NITROPHENOL
2-Morpholinobenzylamine , 97%
1-Boc-4-(4-Bromo-butyl)-piperidine
DL-Leucyl-DL-valine
trans-8-Methyl-6-nonenoyl Chloride
2,5-Dichloro-3-nitropyridine
N-Ethyl-N-(2-hydroxyethyl)perfluorooctylsulphonamide
(S)-(+)-Nipecotic acid
DibroMobis(triphenylphosphine)nickel(II)
2-AMinoethanethiol Hydrochloride
3,4-Diethoxyphenylacetic Acid
Ethyl 2-(4-(4-broMophenylsulfonyl)piperazinoacetate
TrifluoroMethanesulfonic anhydride, 98+%
Prazosin Hydrochloride
2-Morpholino-8-phenylchroMone
2-Nitrobenzamide
octahydrocyclopenta[c]pyrrole
methyl 8-quinolyl ether
10-fluorodecan-1-ol
4-Fluorophenylacetylene
4-Piperidinopiperidine
2-Chlorothiazole
3-Hydroxybenzophenone, 99%
2,2,3,3,3-Pentafluoro-1-propanol, 98%
2-Chloro-3-fluoropyridine
TBTO
3-BroMo-5-fluoropyridin-2-ol
PotassiuM (piperidin-1-yl)Methyltrifluoroborate
Diethylbenzene
2,4-DINITROPHENYL THIOCYANATE
(S)-(-)-2-Pyrrolidone-5-carboxylic acid, 98%
5-[4-(tert-Butyl)phenyl]-2H-1,2,3,4-tetraazole, 97%
5-(2-Bromoethyl)-2,3-dihydrobenzofuran
(4R)-4-amino-1-(tert-butoxycarbonyl)-L-proline(SALTDATA: FREE)
N-Benzylglycine
1-(Dimethylamino)-3-[2-[2-(3-methoxyphenyl)ethyl]phenoxy]-2-propanol hydrochloride
5-Methoxytryptamine hydrochloride
Formic acid
M-Anisic acid
Fluorescein
alpha-Estradiol
2-AMino-4-broMo-6-fluorobenzothiazole
1-(4-Chlorophenyl)ethanol, 98%
NITROBENZENE
L-IsoleucinaMide Hydrochloride
NA
Copper(II) sulfate
Succinic Acid Diammonium Salt
Butylmagnesium chloride
2-CHLORO-4,8-DIMETHYLQUINOLINE
4-(4-Methoxyphenyl)-2,2:6,2-terpyridine, 98%
ACID YELLOW 17
(1S,2S)-1-((4R,11bS)-3H-Dinaphtho[2,1-c:1′,2′-e]phosphepin-4(5H)-yl)-1-phenyl-2-propanaMine
2-Acetylpyridine ,98%
Chloromethyl(2-chlorophenoxy)dimethylsilane
1-(5-Nitrofurfurylidene)aminoguanidine Hydrochloride
Methyl 4-fluoro-2-hydroxybenzoate 98%
Sodium mesitylenesulfonate
3-AMINOMETHYLPHENYLBORONIC ACID, PINACOL ESTER, HCL
5-CHLOROTRYPTAMINE HYDROCHLORIDE
Lavander oil
2-Methoxythiophenol
1,8-Diazafluoren-9-one
4-MaleiMidobutyric Acid
1,4-ButanesultaM
2-BroMo-1-[3-(4-chlorophenyl)-5-isoxazolyl]-1-ethanone, 97%
SUNFLOWER SEED OIL
N-Chloroacetyl-DL-isoleucine
5,6-DIMETHYLTHIENO[2,3-D]PYRIMIDIN-4(3H)-ONE
H-Ala-OiPr.HCl
4-[(morpholinothio)thioxomethyl]morpholine
3-Chloroadamantane-1-carboxamide
Porcine intercellular adhesion molecule 2,ICAM-2 ELISA Kit
Mouse Interferon α,IFN-α ELISA Kit
Human Mucin-5 subtype AC,MUCC ELISA Kit
Human pulmonary activation regulated chemokine,PARC ELISA Kit
T ELISA Kit
S100A8 ELISA Kit
IL-4 ELISA Kit
Human GasR (Gastrin Receptor) ELISA Kit
Human VEGFR-1/Flt1 ELISA Kit
SOX3 ELISA Kit
NBB Broth Base,Modified
Fluid Thioglycollate Medium
Vanillin Isobutyrate
GLYCYL-L-PROLINE
5-Chloro-4-nitrothiophene-2-sulfonyl chloride, 98%
2-Fluorobenzenesulfonyl chloride, 98%
2-Nitrobenzamide
Solvent Yellow 21
Trypsin inhibitor, pancreatic basic
ethyl 4-hydroxy-7-trifluoromethyl-3-quinolinecarb
3-Nitroacetophenone
O-(2-AMINOETHYL)-L-SERINE HYDROCHLORIDE
2,4-Dichloroaniline
Hydroquinone, 99%
5-AcetylsalicylaMide, 98%
3-Methoxy-2-(trifluoroMethyl)benzoic acid, 97%
2-BroMo-5-nitroanisole
L-PenicillaMine
3-PHENYL-1-BUTANOL
1,5-DiMethylpyrazole
Cobalt(II) oxalate, anhydrous, 98%
2,3-Dihydro-1,4-benzodioxine-6-carbothioamide , 97%
(Z)-4-Benzylamino-4-oxobut-2-enoic acid
Indomethacin
2,4,6-Tri(4-pyridyl)-1,3,5-triazine
