U-251 MG细胞：人星形胶质瘤复苏细胞系更专注

U-251 MG细胞：人星形胶质瘤复苏细胞系更专注 货号：GD-C5519276

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-DiMethylaMinobenzoic acid, 98%
DOWEX(R) 50WX4 HYDROGEN FORM
Methyl 2-iodo-4,5-diMethoxybenzoate
3-Nitro-L-tyrosine
BIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)CALCIUM(II)
2-Methoxy-4-nitrophenyl isocyanate
TribroMoacetaldehyde
5-(Chloromethyl)-1-methyl-3-thien-2-yl-1H-pyrazole 97%
N-Methyl-(tetrahydrofuran-3-ylmethyl)amine, 97%
Ethyl bis(phenyl)phosphinate
2,6-Diiodo-4-nitroaniline
2-Methoxy-5-fluorouracil
2-PHENYLBENZYLAMINE HYDROCHLORIDE
alpha-Amyrin
γ-Undecanolactone
Benzo[d]isoxazol-3-ol
2-(DiMethylaMino)vinyl 3-Pyridyl Ketone
3-BroMo-5-fluorobenzaldehyde
Methyl 4-aMinobutyrate hydrochloride, 99%
Sulfurated Potash, LuMp, USP
Ethyl 4-Pyrazolecarboxylate
9-methyl-9H-purin-6-amine
2-chloro-5-Aminophenol
dexamethasone-21-acetate
Rhapontigenin
3,4-(Methylenedioxy)phenylacetonitrile
4-NITROPHENYL BENZOATE
Anthraquinone
6-Chloro-1-hexanol, 97%
4,6-Dichloro-2-(Methylthio)pyriMidine, 98%
SodiuM iodoacetate
M-Chlorotoluene
2,5-DIMERCAPTO-1,3,4-THIADIAZOLE DIPOTASSIUM SALT
O-(2-AMinoethyl)-O′-(2-azidoethyl)heptaethylene glycol
3-tert-Butyl-1-phenyl-2-pyrazolin-5-one, 98%
3-AMino-4-broMopyrazole
Nalpha-Acetyl-L-lysine
Uridine 5'-diphospho-α-D-galactose disodium salt ,95%
2-Methyl-2-nitropropane, 99%
2-Methylpentyl Formate
3'-Hydroxyacetophenone
L-ALANYL-L-TRYPTOPHAN
ACETYLSHIKONIN
DL-CATECHIN
2-Hydroxyethylhydrazine
TriphenylbisMuthine
2,3,4,6-Tetra-O-acetyl-D-Mannopyranosyl Fluoride
2-Methyl-2-butene, 90%, balance 2-Methyl-1-butene
N-BOC-O-Benzyl-L-serine, 98%
L-Cysteine ethyl ester hydrochloride
(1S,2R)-(+)-2-AMino-1,2-diphenylethanol
3-Azabicyclo[3.1.0]hexane-2-carboxylic acid, 3-[(2S)-2-[[(1,...
(3,5-dichloro-pyridin-2-yl)-hydrazine
3,3a,6,6a-tetrahydro-2H-cyclopenta[b]furan-2-one
3-ethyl pyridazine
2,3-Dibromopropionyl chloride
Dichloro(N,N,N′,N′-tetraMethylethylenediaMine)palladiuM(II)
2-AdaMantanaMine hydrochloride
2,2-Bis(4-aMinophenyl)hexafluoropropane
2',5'-Difluoropropiophenone 97%
4-Chloro-2-(trifluoromethyl)benzaldehyde
1-tert-Butoxy-4-chlorobenzene
3,3,5,5-Tetramethyl-1-pyrroline N-Oxide
5-Bromoindazole
Cadmium sulfate
ISOAMYLAMINE
Hydroxypropyl methylcellulose phthalate
Methylene di-p-phenyl diisocyanate
1-Butyl-2,3-diMethyliMidazoliuM Bis(trifluoroMethanesulfonyl)iMide
2,2-Difluorobenzo[1,3]dioxole-4-boronic acid
Creatine phosphate disodiuM salt tetrahydrate, 99%
Methyl 2-aMino-3-(1H-indol-3-yl)propanoate hydrochloride, 97%
N-(Ethoxycarbonyl)nortropinone
4-Chlorobutyryl Chloride
TRIS(TRIMETHYLSILYL)METHANE
(3-fluoro-5-isobutoxy-phenyl)boronic acid
TERT-BUTYLDIMETHYLSILYL (S)-(-)-GLYCIDY
2-methoxyl-4-amino-5-ethylsulfonyl methyl benzoate
DHT ELISA Kit
PSGL-1 ELISA Kit
Human PCDH19 ELISA Kit
Human DPP4 (Dipeptidyl Peptidase IV) ELISA Kit
BAFFR ELISA Kit
Rat Aβ42 (Amyloid Beta 42) ELISA Kit
IGY ELISA Kit
Human EMILIN2 ELISA Kit
SAA3 ELISA Kit
CRY1 ELISA Kit
Power-nitrate Medium
Hemolysis Agar Base
Methyl acetopyruvate, 98%
ScandiuM(III) fluoride, anhydrous, 99.9% (REO)
5,7-Difluoroquinoline
Methyl 2-Furoate
9-methyl-9H-purin-6-amine
Silane, trichloro(3-phenylpropyl)-
Molybdenum(VI) oxide
1,3-Dithiane

2-Cyanoethyl N,N-diisopropylchlorophosphoramidite
Span 60
Fluoroacetyl Chloride
Silver(I) citrate hydrate
D-Valine, 98+%
16,17-alpha Epoxypregnenolone
Patent Blue VF
N-Benzyl 4-broMobenzaMide
4-BroMo-2-fluorobenzenesulfonaMide
3-BROMO-5-ETHOXY-4-HYDROXY-BENZONITRILE
5-BroMo-2-chlorobenzyl alcohol
1-Piperonyl
3-Methyl-1-(4-methylphenyl)-1H-pyrazol-5-ol , 97%
4-(4-Methoxyphenyl)piperidine
2-Methylpyridine-3-boronic acid hydrochloride, 97%