PA-1细胞：人卵巢畸胎瘤复苏细胞系更专注 货号：GD-C5519075
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Allantoin, 98%
Hydroquinine
2-(CARBOXYMETHYLTHIO)-4-METHYLPYRIMIDINE
(1S,2S)-1,2-CYCLOHEXANEDICARBOXYLIC ACID
2-BroMo-4-Methoxy-5-benzyloxybenzoic acid
TRANS-4-METHYL-2-PENTENE
BENZANILIDE
Lithium Acetoacetate
2-Methyl-3-(methylthio)pyrazine
Monopotassium 7-Amino-1,3-naphthalenedisulfonate Hydrate
2,5-Dibromobenzenesulfonamide
1,1,1-Trifluoro-2,3-epoxypropane
GAMMA-L-GLUTAMYL-4-NITROANILIDE
Naphthalene-2,6-disulfonic acid
Methyl 3-aMino-5-phenylthiophene-2-carboxylate
1,3-Thiazol-2-ylMethanol
2,6-DibroMo-p-cresol
N-Hexyl 3-broMo-5-trifluoroMethylbenzenesulfonaMide
TriMethylaMine hydrochloride, 98%
Propylene Glycol Diacetate
Diisopropyl Allylboronate
Methyl 2-bromobenzoate
Z-Glu(OBzl)-OH
(4-Methylphenoxy)acetic acid
Triphenylcarbenium tetrafluoroborate
MOLYBDENUM(V) CHLORIDE
3-Methoxycarbonylphenylboronic acid pinacol ester
2-BroMoiodobenzene
2-Hydroxy-3-Methylpyrazine, 96%
5-Chlorovaleronitrile, 97%
N-Ethyl-N-(2-hydroxyethyl)-p-phenylenediaMine sulfate
4-AMino-4H-1,2,4-triazole
CYCLOHEXYL BUTYRATE
POLY(VINYL ACETATE)
5-(tert-ButoxycarbonylaMino)-1-pentanol , 97%
Trans-(4-Propylcyclohexyl)cyclohex-1-enylboronic acid
Methyl 3-(chlorosulphonyl)-4-phenyl-5-(trifluoromethyl)thiophene-2-carboxylate
Trimethoprim
3-Chloro-3',4'-dimethoxybenzophenone
Dimethyl-n-octylchlorosilane
3,4-Difluoroaniline
3-N-Boc-Aminomethylpiperidine
Polyvinyl chloride
NICKEL AND VANADIUM IN RESIDUAL FUEL OIL
3-Chloro-1-Methyl-1H-pyrazole
TOOS
L-TryptophanaMide Hydrochloride
2-Furaldehyde, 99%
4-Acetylpyridine, 98%
5-ALPHA-ANDROSTANE
2,6-DiMethyl-4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)phenol
5-(Trifluoromethyl)-1-indanone
(R)-1-Cbz-3-Pyrrolidinol
N-tert-Butoxycarbonyl-1,6-hexanediamine
3-nitropyrazole
2-HYDROXYPHENOXYACETIC ACID
5-OXO-2,3-DIHYDRO-1H,5H-PYRIDO[3,2,1-IJ]QUINOLINE-6-CARBALDEHYDE
Glycerol tristearate
alpha-AMinocaproic acid
Tetraphenylarsonium Chloride Hydrochloride Hydrate
5-Bromo-2-hydroxynicotinonitrile
2,4-Dibromo-3,5-difluoroaniline
N-(2-Chlorobenzyloxycarbonyloxy)succinimide, 98%
Tau-fluvalinate
3,3-DIMETHYLHEPTANE
Triadimefon
1,2,3,4-Tetrahydronaphthalene;Tetranap
2,2-DiMethyl-1-propanol
10-BroModecanoic acid
trans-8-Methyl-6-nonenoyl Chloride
5-Ethyl-4h-[1,2,4]triazol-3-ylaMine
1,4-DibroMopentane, 97%
STRONTIUM BROMIDE
Thianthrene
N5-[imino[[(phenylmethoxy)carbonyl]amino]methyl]-N2,N5-bis[(...
6-Bromoisoquinoline
Calcium glubionate
4-Bromophenylboronic acid
SEPE-1 ELISA Kit
Mouse FUCA (Alpha-L-Fucosidase, Tissue) ELISA Kit
Human EGR4 ELISA Kit
Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
Hen egg phosvitin phosphopeptides,PPP ELISA Kit
Smad7 ELISA Kit
Chicken CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) ELISA Kit
Human secretin receptor,SR ELISA Kit
Human Vascular Endothelial cell Growth Factor B,VEGF-B ELISA Kit
AT-1 ELISA Kit
Glucose Peptone Medium
Trypticase Sulfite Neomycin Agar
Isobutyraldehyde, 98%
GLYCYL-L-PHENYLALANINE
5-Chloro-2-Pentanone
2-Fluoro-N,N-diisobutylbenzaMide, 97%
Methyl 2-bromobenzoate
Disperse Red 134
poly(ethylene glycol) monolaurate
5-Amino-2-bromobenzotrifluoride
1,9-DECADIYNE
(1R)-TRANS-1,2-CYCLOPENTANEDIOL MONOACETATE
2,4,6-TrichloropyriMidine
GalliuM(II) telluride, 99.999% (Metals basis)
2-Acetyl-5-broMobenzo[b]furan, 99%
(R)-(+)-3-Boc-2,2-diMethyloxazolidine-4-carboxaldehyde, 95%
6-BroMo-2-(hydroxyMethyl)pyridine
Ethyl D-Lactate
GAMMA-L-GLUTAMYL-4-NITROANILIDE
p-Cresol
PalMitic acid ethyl ester
3-BENZOYLBENZOIC ACID
Rhein, 90%
4,5-Difluorooxindole 97%
4-Chloromethyl-5-methyl-1,3-dioxol-2-one
