GLC-15细胞：人肺癌腺复苏细胞系更专业 货号：GD-C5419248
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(S)-(+)-4-Phenyl-2-oxazolidinone, 98%
HexadecylpyridiniuM chloride Monohydrate
3-(DiMethylaMino)-2-iodoacrolein
2,4-BIS[5,6-DI(P-SULFOPHENYL)-1,2,4-TRIAZIN-3-YL]PYRIDINE TETRASODIUM SALT
2-(tert-ButyldiMethylsilyl)-1,3-dithiane
TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)SAMARIUM(III)
TERT-BUTYLPHOSPHONIC ACID
N-BOC-3-Piperidinecarboxylic acid, 98%
2-(4-Chlorophenoxy)isobutyric Acid
Iron disulfide (Iron pyrite), 95%
Bis(p-sulfonatophenyl)phenylphosphine dihydrate dipotassium salt, min. 97%
Orphenadrine citrate
Imipramine hydrochloride
Propylthiouracil
2-Methylbenzothiazole
Erythrosin B
2,3-Difluorophenetole
Methyl 4-broMo-2-hydroxybenzoate
Zinc phthalocyanine, 96%
StrontiuM Chloride, Hexahydrate, Crystal, Reagent, ACS
DL-Ornithine Monohydrochloride
2-Isopropylaniline
2,3-Dichlorobenzaldehyde
3-Bromo-N-phenylcarbazole
4-Fluorophenylacetonitrile
1-(TRIFLUOROMETHYL)VINYL ACETATE
1,4-DIBROMOBENZENE-D4
N,N'-(1,4-Phenylene)bis(acetoacetaMide)
TerbiuM(III) fluoride, anhydrous, REacton|r, 99.9% (REO)
Ethyl 2-aMino-5-phenylthiophene-3-carboxylate, 97%
1-(3-Methylbenzyl)
Phorate
Dilauryl thiodipropionate
Octocrilene
N,N,N',N'-TETRAETHYL-1,3-PROPANEDIAMINE
PHENANTHRENE-9-CARBOXALDEHYDE
Dichlorobis(tricyclohexylphosphine)palladium(II) ,98%
2,2,2-Trifluoroethanesulphonyl chloride 97%
1,2-Dichloro-1,1-difluoroethane (HCFC-132b) 97%
tert-Butyldimethylsilane
2-IODO-2-METHYLPROPANE
3-CHLORO-4-FLUOROPHENYLACETONITRILE
6-CHLORO-3-INDOLYL-BETA-D-GLUCURONIDE CYCLOHEXYLAMMONIUM SALT
(S)-(+)-2-Amino-3-methyl-1-butanol
Methyl 3-aMino-4-Methylthiophene-2-carboxylate
AMinoMethanesulfonic acid
Heptaethylene Glycol
1-BOC-4-(4-nitrophenyl)
2-Chloro-6-Methylpyrazine, 97%
Potassium bisaccharate
Pyridazine-4-carboxylic Acid
Chloromethyl isopropyl ether
2'-Deoxy-N2-Dimethylaminomethylene-Guanosine
Deoxyadenosine
Dimethylaminohexanol
2,4,5-TRICHLOROBENZENESULFONYL CHLORIDE
(?)-2,3-Bis[(2R,5R)-2,5-diMethylphospholano]-N-MethylMaleiMide(1,5-cyclooctadiene)rhodiuM(I) tetrafluoroborate
N-BENZOYLIMIDAZOLE
(S)-N-FMoc-(3-Pyridyl)alanine
Vanillic Acid
1-(2,4-Difluorophenyl)biguanide hydrochloride 97%
(S,S)-[2-(4'-i-Propyloxazolin-2'-yl)ferrocenyldiphenylphosphine, min. 97%
Pentolinium tartrate, 98%
2-Nonanone
Methyl 2-chloro-4-fluorobenzoate
7-Chloroindole
3-(4-Hydroxyphenyl)propionic acid
3-BroMo-4-Methylbenzonitrile
1-BroMo-4-cyclohexylbenzene
4-Fluoro-3-hydroxybenzonitrile
Chelerythrine chloride, 98%
4-Methoxyphenylacetyl chloride, 98%
4-BroMobenzotrifluoride
6-AMino-1-hexanol
4-hexen-1-ol
Vorapaxar
Fmoc-D-Dab(Boc)-OH
3,5-Dimethyli-4-isoxazolecarboxamide
RXFP1 ELISA Kit
S100β ELISA Kit
Human PCDHβ16 ELISA Kit
Human farnesyl-diphosphate farnesyltransferase 1,FDFT1 ELISA Kit
OGCHI ELISA Kit
BMG/β2-MG ELISA Kit
Chicken anti-Endomysial Antibody IgA,EMA IgA ELISA Kit
CCK-8 ELISA Kit
Human hemagglutinin，HA ELISA Kit
ACH ELISA Kit
Skimmed Milk Sucrose Peptone Medium Base
Alkaline Peptone Water
Diethyl acetylMethylphosphonate, 97%
Hydrogen Fluoride Pyridine CoMplex
5,5'-DibroMo-BAPTA, 95%
2-Fluoro-4-(trifluoroMethyl)pyridine, 97%
2-Isopropylaniline
Quinalphos
Digitonin
Epoxypropionic acid
DIETHYLTIN DICHLORIDE
Nalidixic acid
1-HexadecylpyridiniuM Chloride Monohydrate
2,6-Dichloro-3-nitropyridine, 97%
3,4-DiMethylbenzeneboronic acid, 98+%
1-BroMo-3,4-(Methylenedioxy)benzene
N-Acetyl-L-glutaMic acid
4-(N,N-DiMethylaMinosulfonyl)phenylboronic acid pinacol ester
2-(4-BroMophenyl)-N-MethylacetaMide
2,5-DiMethyl-3(2H)-furanone
2,2'-DIAMINODIPHENYL SULFIDE
3-Hydroxy-4-methoxybenzaldehyde ,99%
2,6-Diacetylpyridine, 99%
Span 85 (=Sorbitan Trioleate)
2-Aminocyclopent-1-ene-1-carbonitrile
