Chang liver细胞：人张氏肝复苏细胞系更专业

Chang liver细胞：人张氏肝复苏细胞系更专业 货号：GD-C5419228

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
DiMethyl 5-aMinoisophthalate, 98%
4-Fluorophenol
4-PHENYL-1-CYCLOHEXENE
4-Hydroxy-3-MethoxycinnaMaldehyde
(R)-(6,6′-DiMethoxybiphenyl-2,2′-diyl)bis[bis(3,4,5-triMethoxyphenyl)phosphine]
cis-1,4-Hexadiene
ETHYL 5-(CHLOROMETHYL)-2-FURANCARBOXYLATE
(-)-1-(9-Fluorenyl)ethyl chloroformate, 0.5 w/v % solution in acetone (ca 18mM)
1-(Hydroxymethyl)adamantane
1-(2-methoxybenzyl)-5-oxopyrrolidine-3-carboxylic acid(SALTDATA: FREE)
5-(3-Chlorophenyl)-1,3-oxazole-4-carboxylic acid
Pigment Red 112
9,10-Bis(phenylethynyl)anthracene
2-BROMO-4-FLUOROBENZENESULFONAMIDE
MethylaMine
1,3-Dithiane
DiaMMoniuM Dithiodiglycolate (45% in Water)
Methyl 2-broMo-4,5-diMethoxybenzoate
YohiMbine hydrochloride, 99%
Sorbitol, Powder, NF
N2-Isobutyrylguanosine Monohydrate
Triacontane
4-Chloro-2-butanone
4-BENZYLBENZENESULFONAMIDE
4-Fluorobenzoic acid
1,2-EPOXY-3-(4-NITROPHENOXY)PROPANE
CIS-CHLORDANE
MagnesiuM Carbonate Hydroxide Pentahydrate
TantaluM nitride, 99.5% (Metals basis)
6-MethylchroMone, 98%
(R)-(-)-8-Diphenylphosphino-1,2,3,4-tetrahydro-1-naphthylaMine, 97+%
Indole-7-carboxaldehyde
3-METHYL-2-BUTANOL
Oxazole-5-carboxylic acid
2-CyclopropylaMinopyriMidine-5-boronic acid, pinacol ester
2-(TRIMETHYLSILOXY)ETHYL METHACRYLATE
2,4-Dichlorophenylhydrazine hydrochloride
3-(Aminomethyl)phenol
2-Dodecylphenol
3,3'-Diethyl-9-methylthiacarbocyanine iodide
Hexyl Acetoacetate
HYDROXYLAMINE
Methyl L-lysinate dihydrochloride
(R)-N-Boc-1-Naphthylalanine
6-(DiethylaMino)-3-pyridinylaldehyde
3'-Hydroxyacetophenone
6-(DibutylaMino)-1,3,5-triazine-2,4-dithiol
N-Diisopropyl 2-broMobenzaMide
trans-Stilbene oxide, 99%
Poly-(a,b)-DL-aspartic acid SodiuM Salt
1-Decanol
1-Bromo-4-propylbenzene
Ethyl 2-Aminothiazole-4-Carboxylate
Di-n-octyltin oxide
1-Bromo-4-propylbenzene
1-BROMO-2-METHYLNAPHTHALENE
2,4-DIBROMO-1-NAPHTHOL
(R)-(-)-2-METHOXY-2-PHENYLETHANOL
(S)-C3-TunePhos
2,4-Dibromo-5-methoxytoluene
4-Amino-2,1,3-benzothiadiazole
5-(Trifluoromethoxy)isatin, 97%
Benzyl methyl sulphone
Pyrazinoic acid hydrazide
2-Methyl-3-nitro-N,N-dipropylphenylacetamide
1-Propanethiol
2-Ethylhexan-1-ol;2-Ethylhexyl alcohol
1-Benzothiophene-7-carbaldehyde
1-BroMo-3,3,3-trifluoroacetone
Ethyl 2,4,6-TriMethylbenzoate
H-Aib-OtBu HCl
2,4,6-Trifluorophenol, 99%
FLUORANTHENE
SulbactaM
N,N-dibutylacetamide
Poly(butadiene)
4,4'-dibromodiphenyl ether
2-cyanopropanoic acid
Porcine myeloperoxidase,MPO ELISA Kit
S100P ELISA Kit
Human FBN1 (Fibrillin 1) ELISA Kit
Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) ELISA Kit
Chicken Interferon β,IFN-β/IFNB ELISA Kit
α-enolase ELISA Kit
CX43 ELISA Kit
Human SLC44A4 ELISA Kit
Human GMP-140 ELISA Kit
ProlAPP ELISA Kit
O/F Medium
LT 100 Agar Base
Benzyl acetoacetate, 97%
TerbiuM(III) fluoride, anhydrous, REacton|r, 99.9% (REO)
5,6-DIAMINO-2,4-DIHYDROXYPYRIMIDINE SULFATE
2-Fluoro-4-Methylpyridine, 98%
Triacontane
(2-Bromoethyl)oxirane
PRASEODYMIUM SULFATE HYDRATE
resveratrol

1,1,1-Trifluoro-2,4-pentanedione
2-AMINOBENZOPHENONE-2'-CARBOXYLIC ACID
4-Fluorobenzonitrile
Ethyl 7-chloroiMidazo[1,2-a]pyridine-2-carboxylate, 95%
Methyl hydrogen succinate, 95%
5-AMino-3-(2-thienyl)-1H-pyrazole, 98%
2-AMino-5-(trifluoroMethyl)pyridine
2,6-Bis[(4R,5R)-4-Methyl-5-phenyl-2-oxazolinyl]pyridine
2-Fluoro-3-trifluoroMethylphenylboronic acid
3-METHYLOXINDOLE 96
Methyl 2-cyclopentanonecarboxylate
Methyl 7-Azaindole-3-glyoxylate
Diethyl adipate, 99%
2,4-Difluoro-alpha,alpha-1-bis(1H-1,2,4-triazol-1-ylmethyl)benzylalcohol
Benzoin Ethyl Ether