B16F10细胞：小鼠黑色素瘤高转移复苏细胞系更专业 货号：GD-C5420300
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
传代培养及其操作步骤：原代细胞培养成功以后，需要进行分离培养，否则细胞会因生存空间不足或密度过大，营养障碍，影响细胞生长。细胞由原培养瓶内分离稀释后传到新的培养瓶中培养的过程称之为传代培养。传代细胞允许培养的细胞扩增(形成细胞株)，可以进行细胞克隆，易于保存，但可能丧失一些特殊的细胞和分化特征。传代细胞形成细胞株的利处在于提供了大量持久的实验材料，便于实验。【操作步骤】1)吸掉或倒掉培养瓶内旧培养液；2)向瓶内加入胰蛋白酶液和EDTA混合液少量。以能覆盖培养瓶底为宜；3)置37℃孵箱或室温(25℃温度)下进行消化，2~5min后把培养瓶放在倒置显微镜下进行观察，当发现胞质回缩、细胞间隙增大后，应立即中止消化；4)吸出消化液，向瓶内加入Hanks液少量，轻轻转动培养瓶，把残留消化液冲掉，然后再加培养液。如果仅使用胰蛋白酶消化，在吸除胰蛋白液后，可直接加入少量含血清的培养液，终止消化；5)使用弯头吸管，吸取瓶内培养液，按顺序反复轻轻吹打瓶壁细胞，使之从瓶壁脱离形成细胞悬液。吹打时动作要轻柔，以防用力过猛损伤细胞；6)用计数板计数后，分别接种于新的培养瓶中，置CO2培养箱中进行培养；7)细胞培养换液时间应根据细胞生长的状态和实验要求来确定。一般2～3d后应换一次生长液。待细胞铺满器皿底面，即可使用；也可继续传代扩大培养或换成维持液。【注意事项】1)掌握好细胞消化的时间，消化时间过短时，细胞不宜从瓶壁脱落，过长的消化会导致细胞脱落、损伤；2)掌握好消化浓度，当消化液浓度过高时，消化时间应缩短，过低时细胞消化时间相对延长。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-AMino-5-broMo-4-Methylpyridine, 97%
4-ForMylbenzoic acid
1-Benzyl-3-pyrrolidinol , 96%
3-Fluoro-2-Methoxyphenol
4-AcetaMidobenzenesulfonaMide
3-(1,2,3,4-Tetrahydroquinolin-2-yl)-2-(trifluoroMethyl) propionic acid
FMoc-N-(2-Boc-aMinoethyl)-Gly-OH
5-Acetyl-2-fluorobenzeneboronic acid
5-Methoxy-4'-(trifluoromethyl)valerophenone
4-(But-3-en-1-yl)chlorobenzene
3-Iodo-2,4,5-trimethylthiophene (stabilized with Copper chip)
2-Chloro-2-methylpropane
BIS(CYCLOPENTADIENYL)RUTHENIUM
Testosterone acetate
3-ForMylphenylboronic Acid
4-Chlorobenzotrifluoride
3,6-Di(2-pyridyl)-1,2,4,5-tetrazine
Undecafluoropentyl Iodide
Tris(4-Methoxyphenyl)phosphine, 95%
Silicotungstic Acid, Reagent
(R)-(+)-2-(MethoxyMethyl)-1-pyrrolidinecarboxaldehyde
3-Diethylamino-1-propanol
Iron(II) oxalate dihydrate
Ethyl 6,8-dichlorocaprylate
Benzoic acid,5-fluoro-2-nitro-, methyl ester
N,N-DIMETHYLFORMAMIDE DINEOPENTYL ACETAL
Ethyl 3-(4-fluorophenyl)-3-oxopropanoate
Benserazide hydrochloride
1-BroMo-4-chloro-2-fluorobenzene, 98%
Di-n-propyl disulfide, 99%
Cys-Gly
2-Nitroanisole
1,2,3-Trifluorobenzene
PHENYLACETIC ACID HYDRAZIDE
1,5-DIHYDROXYANTHRAQUINONE
2-CHLORO-6-IODOTOLUENE
[5-(Trifluoromethyl)-2-pyridinyl]methanol
5-Dibutylamino-naphthalene-1-sulfonyl chloride
2-Dimethylaminobenzylhydrazine dihydrochloride
Diethylaminoacetonitrile
METHYL 5-BROMO-2-FUROATE
4-(1-PYRROLIDINYL)BENZOIC ACID
4-IODOPHENYL ISOTHIOCYANATE
BENZO[A]PYRENE-D12
4-Iodoanisole
2,4,6-TribroMo-3-hydroxybenzoic acid
Trichostatin A
2-Isopropylphenol, 97%
2-Methyl-6,7-diMethoxy-1,2,3,4-tetrahydroisoquinoline hydrochloride, 98%
Polyoxyethylene sorbitan monopalmitate
SodiuM 4-Ethylbenzenesulfonate
Desvenlafaxine Succinate
N-[(9H-Fluoren-9-Ylmethoxy)Carbonyl]-D-Glutamic Acid
5-Chloro-2,3-dihydroxypyridine
2,3-diamino-6-methoxypyridine
1-(4-NITROPHENYL)GLYCEROL
O-PhthaliMide
2-Nitrobiphenyl , 98%
4,5-Dihydro-6-Methylpyridazin-3(2H)-one
N,O-Di-BOC-hydroxylamine, 97%
2-(Piperazin-1-yl)benzoic acid 97%
Benzyltriphenylphosphonium borohydride, 95%
Cresyl Violet perchlorate, laser grade, pure, 99%
METHYL BETA-D-GLUCOPYRANOSIDE
PENTAERYTHRITYL TETRABROMIDE
Cesium iodide
2,6-DiMethyl-4-heptanone;Isobutylketone;Diisopropylacetone;Isovalerone;Valerone
5-BroMo-1-benzothiophene-2-carbonyl chloride
1-AMino-4-broMonaphthalene
Trans-4-(boc-aMinoMethyl)-cyclohexaneMethanaMine
4-BroMo-2-fluorobenzyl cyanide, 98+%
Indole-6-carboxylic acid, 98+%
Methyl hexanoate
Stachydrine Hydrochloride
N,N'-hexamethylenebis(cinnamylideneamine)
Rosuvastatin sodium
Sodium 3-trifluoromethylbenzoate
4-(2-pyrrolidin-1-ylethoxy)aniline
DEHA-S ELISA Kit
COL6α1 ELISA Kit
Human APOE ELISA Kit
Human DEFβ125 ELISA Kit
Chicken Leukemia inhibitory factor,LIF ELISA Kit
Rat HRAS (V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog) ELISA Kit
PRL/LTH ELISA Kit
Human LMP7 ELISA Kit
Human ANGPTL1 ELISA Kit
SAA ELISA Kit
PPLO Agar Base
Tetracyline Examination Medium
IsopropylMagnesiuM Chloride
FuMaryl chloride, 95%
5-Chloro-2,4-Difluoroaniline
2-Fluoro-5-bromopyridine
3-Diethylamino-1-propanol
Solvent Black 27
ecteola cellulose fine mesh
3-bromo-4-chlorobenzotrifluoride
3-Chloro-4-fluorophenylboronic acid
2,8-BIS(TRIFLUOROMETHYL)-4-CHLOROQUINOLINE
Crotonyl alcohol
BariuM sodiuM niobiuM oxide, Puratronic|r, 99.999% (Metals basis)
1,3-Bis(trifluoroMethyl)benzene, 98+%
Benzyl 3-broMopropyl ether
2-Fluoro-3-pyridineboronic acid
6-(2-Thienyl)-2-pyridinecarboxaldehyde
3,5-Diacetoxyacetophenone
11-Azido-1-undecanethiol
3-FLUORO-4-METHYLPHENOL
4-Amino-3-(trifluoromethyl)pyridine
trans-(4-Ethoxybenzoyl)-3-acrylic acid, 98%
1H,1H,2H,2H-Perfluorooctylmethyldimethoxysilane 97%
4-(5-Oxopyrazolidin-3-yl)benzeneboronic acid 96%
