PC-3MIE8细胞：人前列腺癌高转移复苏细胞系技术强 货号：GD-C5319055
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2,2'-Dichloro-3,3'-bipyridine, 94%
2,6-Dichlorophenylacetonitrile
4-tert-Butyl-N,N-diMethylaniline
1,1'-BIS(DICHLOROPHOSPHINO)FERROCENE
N'-BENZYL-N,N-DIMETHYLETHYLENEDIAMINE
2,3,4,5-TETRAMETHYL-2-CYCLOPENTENONE
1,10-Phenanthroline hydrate
2,3,5,6-Tetrafluorobenzonitrile
Tetramethylbiphosphine disulfide, 99%
Amberlyst? A-21, ion-exchange resin
5-Methyl-1-hexyn-3-ol
Salidroside
Cefotaxime
Celite
2-BroMo-1-(4-pentylphenyl)ethan-1-one
2-ChloropyriMidine
2,2-DiMethyl-1,3-benzodioxole
Ethyl 5-broMopicolinate
TrifluoroMethanesulfonyl chloride, 99%
Sulfur AtoMic Absorption Standard
4'-Ethoxybenzylidene-4-butylaniline
Dimethyl 4-nitrophthalate
4-phenylazetidin-2-one
2-Amino-4-hydroxy-1H-pteridine
1,4-dibromooctafluorobutane
1-Bromo-4-nitrobenzene
N-Benzyloxycarbonyl-L-proline
N-Thionylaniline
1,1':3',1''-Terphenyl-5'-boronic acid, 95%
2,5-DibroMothiazole, 97%
2-(2-Hydroxyphenyl)benzothiazole
4-(N-Boc-aMino)phenylboronic acid
3-(4-BroMophenyl)-1-(4-chlorophenyl)pyrazole-4-propionic acid
PotassiuM (4-Methylsulfonylphenyl)trifluoroborate
Benz[b]indeno[1,2-e]pyran-6-carboxaldehyde
trans-DichlorodiaMineplatinuM(II)
5-Chloro-2-methyl-benzothiazole
Perfluorooctanamide
6-Chloro-5-(chloroacetyl)-1,3-dihydro-2H-indol-2-one 97%
4-Chloro-3-formamidobenzotrifluoride
Gadolinium oxide
TETRAETHYLTIN
Sodium lauroylsarcosinate
SodiuM Benzoate
LithiuM hydride
2,3-DibroMopropene
Ethyl 4-Pentenoate
Ac-Lys(Boc)-OH
(R)-(+)-tert-ButylsulfinaMide, 98%
ETHYLBENZENE
(-)-2,3:4,6-Di-O-isopropylidene-2-keto-L-gulonic Acid Monohydrate
2-aminoimidazole sulfate
Fatty alcohol polyoxyethylene ether
Diethyl allylmalonate
3',6'-dichlorospiro(phthalide-3,9'-xanthene)
2,5-Difluoronitrobenzene
6-METHOXY-2-NAPHTHALENEBORONIC ACID
1-BOC-pyrrole-3-boronic acid, pinacol ester
N-Acetyl-3,5-diiodo-L-tyrosine
N-(Hydroxymethyl)benzamide
2-Bromo-5-chlorophenylacetic acid
Barium 2-ethylhexoxide (~1M in hexane/toluene)
Phenyldichlorosilane
CHALCONE
2'-Methoxyacetophenone
N,N-Dimethylacrylamide
Hexacosane
5-Pyridin-2-yl-1H-pyrazole-3-carboxylic acid
1,8-Dichloro-9,10-bis(phenylethynyl) anthracene
2-NitrocuMene
1H-Isoindol-3-aMine
Ethyl 3-(4-hydroxyphenyl)propanoate, 97%
Magnesium ascorbyl phosphate
2,4-Difluoroanisole
Z-Nva-OH
1-(aminomethyl)cyclopentanol
cromolyn sodium
2,4,6-Trimethylphenethylalcohol
ICAM-2/CD102 ELISA Kit
Mouse Interferon α,IFN-α ELISA Kit
Human Mucin-5 subtype AC,MUCC ELISA Kit
Human Pulmonary surfatcant-associated protein D,SP-D ELISA Kit
SCF ELISA Kit
S100A9 ELISA Kit
IL-4 ELISA Kit
Human gastrin receptor，GsaR ELISA Kit
KDR ELISA Kit
SHBG ELISA Kit
Thioglycollate Broth（Enriched）
Clostridium Welchii Agar Base without Kanamycin
Vanillin isobutyrate
1,2-DIOLEOYL-RAC-GLYCEROL
5'-Chloro-4'-fluoro-2'-nitroacetanilide, 98%
2-Fluorobenzenesulfonyl chloride
Dimethyl 4-nitrophthalate
Basic Blue 57
elastase from human leukocytes
7-Trifluoroisatin
PHENYLSILANE
3,4,5-Trimethoxyphenylboronic acid
2,5-Dichlorothiophene
1-Boc-indole-2-boronic acid, 95%
2-Chloro-5-(trifluoroMethyl)benzoyl chloride, 97%
2-BroMo-3,5-dichloropyridine
1-Ethyl-3-MethyliMidazoliuM bis(trifluoroMethylsulfonyl)iMide
5-BroMo-2-chloronicotinic acid
Methyl 4-Methylsalicylate
N-BOC-D-Arginine hydrochloride
E-Hexen-1-ylboronic acid
4-Bromo-2-methylphenol
1,2-Dicyano-3,4,5,6-tetrafluorobenzene
Terbium(III) chloride, anhydrous (99.9%-Tb) (REO)
(2R)-2-Aminopropanamide hydrochloride
