ECC10细胞:人子宫内膜癌复苏快细胞系 货号:GD-C4720140
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Diethyl oxalate, 99%
SalicylhydroxaMic acid
S-(2-Phenoxyethyl)thioacetate
PalladiuM hydroxide
D-ALPHA-TOCOPHERYLQUINONE
1H-Indazole-6-carboxaldehyde
Mono-Methyl terephthalate
3-Methacryloxypropyltris(methoxyethoxy)silane tec(3-Tris(2-methoxyethoxy)silyl propylmethacrylate
1-(4-Methoxy-pyridin-2-yl)-ethanone
1,2,3,4,6-Penta-O-acetyl-α-D-galactopyranose ,98%
Hexamethyleneammonium Hexamethylenedithiocarbamate
Bromoxynil
21-DEOXYCORTISONE
3,4-Difluorobenzotrifluoride
4-Piperidinobenzoic acid
4-Chlorophenylacetone
trans-N,N'-Diacetylcyclohexane-1,2-diaMine
2,4-Dichlorobenzyl broMide
trans-4-Phenyl-3-buten-2-one, 99%
PIPES DipotassiuM Salt, Biological Buffer
Ethyl Anthranilate
L-m-Tyrosine
4,7-dichloro-1,10-phenanthroline
D-Pantothenic Acid Sodium Salt
β-Phenylethylamine hydrochloride
PYRAZINE-N,N'-DIOXIDE
4-Fluorophenoxyacetic acid
2-Ethylbutyl acetate, 97%
3-Chloro-2,6-difluorobenzoic acid, 97%
D-GlutaMic acid, 99+%
Geranyl chloride
N-tert-Butyl-3-MethoxybenzaMide
(3S,5R,8aS)-(+)-Hexahydro-3-phenyl-5H-oxazolo[3,2-a]pyridine-5-carbonitrile
Pyridine sulfur trioxide
2-AcetaMido-4-fluorobenzoic acid
3-Chlorophenyl isocyanate
N-(3-Aminopropyl)-N-methylaniline
alpha,alpha-dibromo-4'-fluoroacetophenone
trans-3-Amino-1-Boc-4-methoxypyrrolidine
(3-Bromobenzyl)dimethylamine
Sodium 3,5,6-trichloropyridin-2-olate
3-METHYL-1-PHENYL-2-PHOSPHOLENE 1-OXIDE
S-BUTYRYLTHIOCHOLINE IODIDE
Granisetron Related CoMpound D;1-Methyl-1H-indazole-3-carboxylic acid
2-Methyl-2-[(7-Methyl-2,3-dihydro-1H-inden-4-yl)oxy]propanoic acid
Sulfonazo III [SpectrophotoMetric reagent for alkaline earth Metals and indicator for the precipitation titration of SO4 with Ba]
5-Hydroxy-3,4-dihydro-2(1h)-quinolinone
4-Methylsulphonylphenol, 95%
3-Phenoxybenzoyl chloride, 97%
2-Amino-4,6-dimethylpyrimidine
Acetoxy-2-propanone (stabilized with Mg6Al2(OH)16CO3.4H2O)
Cyproterone acetate
methyl-2,3-dideoxy-3-fluoro-5-o-(4-phenylbenzoyl)-alpha-d-erythro-pentofuranoside
Valproate semisodium
4-Isopropylbenzenesulphonyl chloride
2-BENZYLIDENECYCLOHEXANONE
SodiuM Ligninsulfonate
2-Fluoro-4-(trifluoroMethyl)pyridine
Phthaloyl dichloride
2,2,3,3,4,4,4-Heptafluoro-1-butanol, 95%
4-Benzyl-3-hydroxymethylmorpholine
Hydroxypropyl methylcellulose phthalate 55
Potassium (E)-propenyl-1-trifluoroborate
4-(TRIMETHYLSILOXY)BENZALDEHYDE
N6-Cbz-L-Lysine benzyl ester hydrochloride
2,6-Dichloroquinoxaline
2,5-DiMethylthiophene;2,5-Thioxene; α,α′-Thioxene;
AMaranth
1,4-Bis(3-hydroxyphenoxy)benzene
2-(t-ButylaMino)sulfonylphenylboronic acid
alpha-Methyl-3-(trifluoroMethyl)benzyl alcohol, 96%
N1-(5-AMino-2-Methylphenyl)acetaMide, 97%
6-Phenyl-2-pyridone
Ethylgonendione
N,N-dibutylaniline
1H-Indole-1-carboxylicacid, 5-bromo-2,3-dihydro-, 1,1-dimethylethyl ester
5-bromo-D-tryptophan
(Z)-2'-(1H-imidazol-1-yl)-2,4-dichloroacetophenone oxime
CKMT1A ELISA Kit
Mouse β-Defensins,β-DF ELISA Kit
Human ITGA5 ELISA Kit
sPLA2 ELISA Kit
AmAC-1 ELISA Kit
Rat MAPK (P38 Mitogen-Activated Protein Kinase) ELISA Kit
IFN-α ELISA Kit
Human PLXND1 ELISA Kit
Human AGTR2 ELISA Kit
FLNa ELISA Kit
Yeast Synthetic Drop-out Agar Medium
Wallerstein Laboratory Nutrient Agar
Isododecane, Mixture of isoMers, tech. 80%
Iron(II) perchlorate hydrate, Reagent Grade
Ethyl 5-Chloro-2-Indolecarboxylate
SODIUM GLYCEROPHOSPHATE HYDRATE
L-m-Tyrosine
4-acetoxy-3-methoxybenzoic acid
Silver bromide
3-fluoro-4-methoxybenzonitrile
2-BROMO-5-NITROBENZOIC ACID
4-Isopropylbenzeneboronic Acid
p-Benzoquinone dioxiMe, 95%
1-Acetylpiperidine-4-carboxylic acid, 98+%
StrontiuM, distilled dendritic pieces, 99.8% (Metals basis)
HexadecylpyridiniuM broMide
Butyl butyrate
(R)-(?)-HexahydroMandelic acid
4-Cyanophenyl isocyanate
Ethylboronic acid
3,4-Difluorophenylglyoxal hydrate, 98%, dry wt. basis
2,6-Difluoro-4-methoxybenzonitrile
Zinc stannate
Ethyl 1-(2,4-difluorophenyl)-7-Cl-6-F-4-O-hydropyridino[2,3-b]pyridine-3-carboxylate, 99%
3-(Bromomethyl)-1,2-benzisoxazole