RF952细胞:人子宫内膜癌复苏快细胞系 货号:GD-C4720083
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
1-BroMo-3-nitro-4-(trifluoroMethoxy)benzene, 95%
(±)-2-Methyl-1-butanol
3-PHENOXYPHENYLACETONITRILE
4-TERT-OCTYLRESORCINOL
7-Chloro-4-iodoquinoline
(1R,2R)-TRANS-1,2-CYCLOHEXANEDIOL
4-Azatricyclo[4.3.1.1~3,8~]undecan-5-one hydrochloride
3-(1,3-dihydro-2H-isoindol-2-yl)benzoic acid(SALTDATA: FREE)
Methyl imidazo[1,2-a]pyrazine-8-carboxylate
1-(2-Bromo-ethyl)-3-methyl-benzene
2-Chloro-6-fluoro-4-(trifluoromethyl)phenol 98%
2-[(4,6-DIMETHOXYPYRIMIDIN-2-YL)THIO]BENZOIC ACID
METHYL 2-FLUOROBENZOATE
3-(2,4-DICHLOROPHENYL)PROPIONIC ACID
Methyl 1-aminocyclopropanecarboxylate hydrochloride
TetrabutylaMMoniuM hydrogensulfate
Di-n-octyl Sulfoxide
2,4-DibroMo-5-fluoroanisole
Tin(IV) acetate, 98%, extra pure
Papaverine Hydrochloride, USP
Iron(II) Phthalocyanine
2,5-Dichlorophenol
Silane coupler KH-791
5-Amino-4-bromobenzothiazole
DL-Pyroglutamic acid
HEXAHYDRATE
1-(3-BroMophenyl)
2-BroMo-4-fluoro-N-(3-fluorobenzyl)aniline, 97%
2-Chloro-3,5-dinitrobenzoic acid, 97% (dry wt.), May cont. up to ca 5% water
3-(4-BroMophenyl)-1H-pyrazole, 97%
3-BroMo-2-nitropyridine
TRANS,CIS-2,6-NONADIEN-1-OL
2,3,5,6-Tetrafluoro-4-hydroxybenzoic acid hydrate
RuCl2[(S)-(DM-BINAP)][(S,S)-DPEN]
6-BroMo-7-Methyl-3-nitroiMidazo[1,2-a]pyridine
DL-Asparagine Monohydrate
Hexaphenylcyclotrisiloxane
2,3:4,5-Di-O-isopropylidene-beta-D-fructopyranose
(4'-Methoxy-biphenyl-3-yl)-acetic acid
2-(Aminomethyl)-5-fluorobenzeneboronic acid hydrochloride
2-(2-METHYLPHENYL)PYRROLIDINE
5-Methylnicotinic acid
2,6-Dinitro-p-cresol
4-Amino-m-cresol
2-Phenyl-2-iMidazoline
4-(2-BroMoethyl)benzenesulfonic Acid
4-chloro-2-fluorophenylboronic aicd, pinacol ester
beta-Tetralone, 95%
Ethyl dichlorophosphite, 98%
3-AMino-1-tert-butoxycarbonylpiperidine
3-AMino-1-propanol Hydrochloride
1-Adamantanamine hydrochloride
Cyclopenta[c]pyrrole-1-carboxylic acid, (2S)-2-cyclohexyl-N-(pyrazinylcarbonyl)glycyl-3-methyl-L-valyloctahydro-, ethyl ester, (1S,3aR,6aS)-
(2S)-2-(tert-butylcarbamoylamino)-3,3-dimethyl-butanoic acid
2,2-Bis[4-(4-aminophenoxy)phenyl]-hexafluoropropanane
DIPROPYL PHTHALATE
2-PhthaliMidopropionic acid
1-N-HEPTYLOXY-4-NITROBENZENE
2-Methyl-3-nitroaniline
6-Methoxy-1H-pyrrolo[2,3-b]pyridine
2-(2-Chloroethoxy)tetrahydro-2H-pyran
1-(tert-Butoxycarbonyl)pyrrolidine-2-carboxylic acid
5-methyl-1,3-diphenyl-1H-pyrazole-4-carboxylic acid(SALTDATA: FREE)
n-Octyl Sulfide
Metazachlor
Butyl lactate
Allyl mercaptan
2-Methoxy-5-fluoropyridine
1,3-Bis(trifluoroMethyl)-5-broMobenzene
N-[3-[3-(DiMethylaMino)acryloyl]phenyl]-N-ethylacetaMide
4-(2-Chlorophenyl)benzaldehyde
1,2-DibroMocyclopentene, 97%
Xylazine Hydrochloride, USP
3,5-DibroMopyridine
Methallyl acetate
Ethyl 4-formyl-1H-pyrrole-2-carboxylate
Calcium folinatc
Decamethylcyclopentasiloxane
SEMA4D/CD100 ELISA Kit
Mouse β-glucuronidase,βGD ELISA Kit
Lp-PLA2 ELISA Kit
CoQ10 ELISA Kit
ANXA5 ELISA Kit
Rat L-Phenylalanine ammonla-lyase,PAL ELISA Kit
TRAIL-R3 ELISA Kit
Human PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
Human Trichinella antibody ELISA Kit
PGC ELISA Kit
Schwartz Differential Medium
Pseudomonas Agar for Determine of Pyocyanin
YtterbiuM sputtering target, 76.2MM dia x 1.59MM thick
ChroMotrope FB
5-AMino-2-cyano-3-Methoxypyridine, 96%
2-Methyl-1,3-dithiane, 98%
2,5-Dichlorophenol
Methyl 3-hydroxyisonicotinate
Methyl-D3-p-Toluenesulfonate
Sulfadimethoxypyrimidine
Prenyl acetate
(Trifluoromethyl)trimethylsilane
Triphenyltin Acetate
2,3-Dihydroxypyridine, 98%
CyclopropyldiphenylMethanol, 98+%
4-Chloro-4'-hydroxybenzophenone
BroMoethane
2-(CYCLOHEXYLAMINO)PYRIDINE-5-BORONIC ACID PINACOL ESTER
0
Methyl 9-fluorenylideneacetate
Benzene, 1,3,5-trifluoro-2-Methoxy- (9CI)
1,3-Bis(3-aminopropyl)tetramethyldisiloxane ,97%
3-Methyl-5-phenyl-4-isoxazolecarboxylic acid, 97%
2,4-Diacetyl deuteroporphyrin IX dimethyl ester
4-(Trifluoromethyl)benzophenone 97%