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RF952细胞:人子宫内膜癌复苏快细胞系

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  • RF952细胞:人子宫内膜癌复苏快细胞系 货号:GD-C4720083
    细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
    产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
    产品规格:≥1*10(6)/ml
    安全级别:1
    物种来源:人源或鼠源等
    细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
    接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
    细胞特性:贴壁/悬浮等
    细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
    培养条件:详见细胞说明书
    传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
    1-BroMo-3-nitro-4-(trifluoroMethoxy)benzene, 95%
    (±)-2-Methyl-1-butanol
    3-PHENOXYPHENYLACETONITRILE
    4-TERT-OCTYLRESORCINOL
    7-Chloro-4-iodoquinoline
    (1R,2R)-TRANS-1,2-CYCLOHEXANEDIOL
    4-Azatricyclo[4.3.1.1~3,8~]undecan-5-one hydrochloride
    3-(1,3-dihydro-2H-isoindol-2-yl)benzoic acid(SALTDATA: FREE)
    Methyl imidazo[1,2-a]pyrazine-8-carboxylate
    1-(2-Bromo-ethyl)-3-methyl-benzene
    2-Chloro-6-fluoro-4-(trifluoromethyl)phenol 98%
    2-[(4,6-DIMETHOXYPYRIMIDIN-2-YL)THIO]BENZOIC ACID
    METHYL 2-FLUOROBENZOATE
    3-(2,4-DICHLOROPHENYL)PROPIONIC ACID
    Methyl 1-aminocyclopropanecarboxylate hydrochloride
    TetrabutylaMMoniuM hydrogensulfate
    Di-n-octyl Sulfoxide
    2,4-DibroMo-5-fluoroanisole
    Tin(IV) acetate, 98%, extra pure
    Papaverine Hydrochloride, USP
    Iron(II) Phthalocyanine
    2,5-Dichlorophenol
    Silane coupler KH-791
    5-Amino-4-bromobenzothiazole
    DL-Pyroglutamic acid
    HEXAHYDRATE
    1-(3-BroMophenyl)
    2-BroMo-4-fluoro-N-(3-fluorobenzyl)aniline, 97%
    2-Chloro-3,5-dinitrobenzoic acid, 97% (dry wt.), May cont. up to ca 5% water
    3-(4-BroMophenyl)-1H-pyrazole, 97%
    3-BroMo-2-nitropyridine
    TRANS,CIS-2,6-NONADIEN-1-OL
    2,3,5,6-Tetrafluoro-4-hydroxybenzoic acid hydrate
    RuCl2[(S)-(DM-BINAP)][(S,S)-DPEN]
    6-BroMo-7-Methyl-3-nitroiMidazo[1,2-a]pyridine
    DL-Asparagine Monohydrate
    Hexaphenylcyclotrisiloxane
    2,3:4,5-Di-O-isopropylidene-beta-D-fructopyranose
    (4'-Methoxy-biphenyl-3-yl)-acetic acid
    2-(Aminomethyl)-5-fluorobenzeneboronic acid hydrochloride
    2-(2-METHYLPHENYL)PYRROLIDINE
    5-Methylnicotinic acid
    2,6-Dinitro-p-cresol
    4-Amino-m-cresol
    2-Phenyl-2-iMidazoline
    4-(2-BroMoethyl)benzenesulfonic Acid
    4-chloro-2-fluorophenylboronic aicd, pinacol ester
    beta-Tetralone, 95%
    Ethyl dichlorophosphite, 98%
    3-AMino-1-tert-butoxycarbonylpiperidine
    3-AMino-1-propanol Hydrochloride
    1-Adamantanamine hydrochloride
    Cyclopenta[c]pyrrole-1-carboxylic acid, (2S)-2-cyclohexyl-N-(pyrazinylcarbonyl)glycyl-3-methyl-L-valyloctahydro-, ethyl ester, (1S,3aR,6aS)-
    (2S)-2-(tert-butylcarbamoylamino)-3,3-dimethyl-butanoic acid
    2,2-Bis[4-(4-aminophenoxy)phenyl]-hexafluoropropanane
    DIPROPYL PHTHALATE
    2-PhthaliMidopropionic acid
    1-N-HEPTYLOXY-4-NITROBENZENE
    2-Methyl-3-nitroaniline
    6-Methoxy-1H-pyrrolo[2,3-b]pyridine
    2-(2-Chloroethoxy)tetrahydro-2H-pyran
    1-(tert-Butoxycarbonyl)pyrrolidine-2-carboxylic acid
    5-methyl-1,3-diphenyl-1H-pyrazole-4-carboxylic acid(SALTDATA: FREE)
    n-Octyl Sulfide
    Metazachlor
    Butyl lactate
    Allyl mercaptan
    2-Methoxy-5-fluoropyridine
    1,3-Bis(trifluoroMethyl)-5-broMobenzene
    N-[3-[3-(DiMethylaMino)acryloyl]phenyl]-N-ethylacetaMide
    4-(2-Chlorophenyl)benzaldehyde
    1,2-DibroMocyclopentene, 97%
    Xylazine Hydrochloride, USP
    3,5-DibroMopyridine
    Methallyl acetate
    Ethyl 4-formyl-1H-pyrrole-2-carboxylate
    Calcium folinatc
    Decamethylcyclopentasiloxane
    SEMA4D/CD100 ELISA Kit
    Mouse β-glucuronidase,βGD ELISA Kit
    Lp-PLA2 ELISA Kit
    CoQ10 ELISA Kit
    ANXA5 ELISA Kit
    Rat L-Phenylalanine ammonla-lyase,PAL ELISA Kit
    TRAIL-R3 ELISA Kit
    Human PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
    Human Trichinella antibody ELISA Kit
    PGC ELISA Kit
    Schwartz Differential Medium
    Pseudomonas Agar for Determine of Pyocyanin
    YtterbiuM sputtering target, 76.2MM dia x 1.59MM thick
    ChroMotrope FB
    5-AMino-2-cyano-3-Methoxypyridine, 96%
    2-Methyl-1,3-dithiane, 98%
    2,5-Dichlorophenol
    Methyl 3-hydroxyisonicotinate
    Methyl-D3-p-Toluenesulfonate
    Sulfadimethoxypyrimidine

    Prenyl acetate
    (Trifluoromethyl)trimethylsilane
    Triphenyltin Acetate
    2,3-Dihydroxypyridine, 98%
    CyclopropyldiphenylMethanol, 98+%
    4-Chloro-4'-hydroxybenzophenone
    BroMoethane
    2-(CYCLOHEXYLAMINO)PYRIDINE-5-BORONIC ACID PINACOL ESTER
    0
    Methyl 9-fluorenylideneacetate
    Benzene, 1,3,5-trifluoro-2-Methoxy- (9CI)
    1,3-Bis(3-aminopropyl)tetramethyldisiloxane ,97%
    3-Methyl-5-phenyl-4-isoxazolecarboxylic acid, 97%
    2,4-Diacetyl deuteroporphyrin IX dimethyl ester
    4-(Trifluoromethyl)benzophenone 97%
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