ASH-3细胞：人未分化甲状腺癌复苏快细胞系 货号：GD-C4720033
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明：请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式：T25培养瓶(一般是贴壁细胞使用，悬浮细胞也可以)、离心管(悬浮细胞使用多，当然，有些公司也会用这种离心管形式发贴壁细胞，但是，长期经验来说，贴壁细胞容易发生形态变化，估计是细胞培养空间狭小，血清不足导致(Z主要是怕运输延误导致这些情况发生)，个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用，主要是冬天天气冷，温度低于4℃的地方，都要考虑冻存细胞)。T25培养瓶：是用T25细胞培养瓶直接发货的形式。收到细胞后，拆开包装T25培养瓶的自封袋，不拆封口膜，表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用，如细胞密度高于80%，可以直接消化传代，相反则加入5-6ml培养基放回培养箱继续培养即可。离心管：是用15ml离心管发货的形式，只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶，可以暂时T75培养瓶，加入10-15ml培养基，建议10ml培养基，也可以使用T175培养瓶，加入50-60ml培养基，培养瓶越大，需要的培养基越多。当然如果刚接收到细胞密度不够的话，Z好不用大瓶子，先用小瓶子养起来再换大的，不然会长得很慢，严重的，会导致细胞死亡等危险)，平衡完成后，离心(1000rpm，3min)收集细胞，弃上清，用新的培养基重悬细胞并接种至培养瓶或皿中，放回培养箱继续培养。干冰：是用本公司冻存液冻存细胞后，直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
N-Benzyl-3-broMo-5-(trifluoroMethyl)benzenesulfonaMide, 97%
1-BroMo-6-chlorohexane
NICOTINOYL CHLORIDE HYDROCHLORIDE
4-Nitrophenyl beta-D-galactopyranoside
2-Chloropyridine-3,4-diaMine
4-(Methoxycarbonyl)furan-2-boronic acid, pinacol ester
1-(3-BroMophenylsulfonyl)piperidine
3-Nitrodiphenyl
N-(3-Aminopropyldimethylsila)aza-2,2-dimethyl-2-silacyclopentane
NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
2-Bromo-6-chloro-4-nitroaniline
2-(2'-FLUOROPHENYL)-ACETYL-CHLORIDE
MAGNESIUM MONOPEROXYPHTHALATE HEXAHYDRATE
METHYL PHENYL SULFOXIDE
3-Thiocyanatopropyltriethoxysilane
Phosphorus oxybroMide
4-(Dodecyloxy)benzoic Acid
N-(3-Chloropropyl) 4-broMobenzenesulfonaMide
Tetra-n-butylaMMoniuM trifluoroMethanesulfonate, 99%
N-Phenylglycine
2-Chloropyridine-5-boronic Acid (contains varying aMounts of Anhydride)
Acetomenadione
4-[(1E)-3-(2,4-dihydroxyphenyl)-3-oxoprop-1-en-1-yl]phenyl beta-D-glucopyranoside
cyclohexanecarbonitrile
2-Nitroso-1-naphthol
2,2,2-Trifluoroethyl methacrylate
METHYL 4-ACETYLBENZOATE
1-(3,4-DiMethylphenyl), 97%
L-Cysteic acid Monohydrate, 99%
4-Nitrothioanisole, 98%
1-Hexanesulfonyl chloride
(+)-L-TARTARIC ACID DIBENZYL AMIDE
N-(2,6-DIETHYLPHENYL)MALEIMIDE
Silica gel
3,4-DiMethylbenzylaMine
Methyl 1-(5-broMopyridin-2-yl)-5-cyclopropylpyrazole-4-carboxylate
3-Chlorobenzo[b]thiophene-2-carbonyl chloride , 97%
4-Cyanophenyl 4-propyl-benzoate
1,3-Diiminoisoindoline, 97%
1-Ethynyl-4-heptylbenzene
P-XYLENE
Benzyltributylammonium bromide
4-TERT-BUTYLPHENYLHYDRAZINE HYDROCHLORIDE
TeMozoloMide Related CoMpound A;4-diazo-4H-iMidazole-5-carboxaMide
2-BroMophenylacetic acid
2-Benzoylacetanilide
2-BroMo-6-fluoro-3-propoxyphenylboronic acid
6-Ethyl-o-toluidine, 98+%
4-Fluoro-2(trifluoroMethyl)benzoic acid, 98%
SodiuM Hexadecyl Sulfate (contains ca. 40% SodiuM Stearyl Sulfate)
1-Chloro-1-propene (cis- and trans- Mixture)
Dimethyl oxalate
Sodium 1-pentanesulphonate monohydrate
Erdosteine
Chloroplatinic Acid
5-Bromo-7-nitroindoline
N-alpha-Cbz-L-lysine
2,6-Dichloro-4-(trifluoroMethyl)pyridine
(S)-(?)-AMinoglutethiMide
2-Amino-4-hydroxy-6-phenylpyrimidine
4-Methylumbelliferyl-B-D-glucuronic acid
2-Chloro-5-nitropyrimidin-4-amine ,95%
2,6-Diisopropylnaphthalene
Leaf acetate
9-ACRIDINECARBOXYLIC ACID HYDRATE
DI-N-BUTYL SULFOXIDE
1,9-DibroMononane;NonaMethylene dibroMide;NonaMethylene broMide
YohiMbine hydrochloride
1,3,5-AdaMantanetriol
L-2-Thienylalanine
4-AMinophenyl sulfone, 97%
5-AzabenziMidazole, 97%
DYSPROSIUM(III) CHLORIDE HEXAHYDRATE
4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one
trimethobenzamide
EPTAPLATIN
Hydride terminated methyhydrosiloxane dimethylsiloxane copolymer
Diphacinone Sodium
ATA ELISA Kit
γGT1 ELISA Kit
FABP4 ELISA Kit
Human ACAST-DⅣ ELISA Kit
LEP ELISA Kit
FCAR ELISA Kit
TNF-α ELISA Kit
FGFBP2 ELISA Kit
Human Tα1 (Thymosin-α1) ELISA Kit
HAase ELISA Kit
Martin Medium
Azospirillum Amazonense Medium
Methyl indole-5-carboxylate
Methyl heptanoate
5-Aminosalicylic acid
Propyl 2-Methyl-3-furyl disulfide
Acetomenadione
Tetrahydropalmatin
6-bromonaphthalen-2-amine
Trisodium edetate
EU(FOD)3
1,3-Bis(4-piperidyl)propane
2-Fluoro-6-Hydroxybenzonitrile
2,2-Difluoro-1,3-benzodioxole-4-carboxylic acid, 97%
7-BroMo-5-fluoroindole, 97%
α-Sophorose Monohydrate
NapropaMide Standard
LEUCOMALACHITE GREEN
(1S,2R)-(-)-cis-1-AMino-2-indanol
FERRIC(III) HEXAFLUOROACETYLACETONATE
N-FORMYL-D-PHENYLALANINE
2,4-Dimethoxyphenylboronic acid ,98%
Hexyl Methanesulfonate
2,3-Dichlorophenethylamine, 99+%
Fmoc-HomoArg(Pbf)-OH
