FRO细胞：人未分化甲状腺癌复苏快细胞系 货号：GD-C4718259
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-(4-Piperidinyloxy)acetaMide, 99%
5-BroMo-2-ethoxypyridine
Hydroquinidine
TantaluM wire, 2.0MM (0.08in) dia, annealed, 99.95% (Metals basis excluding Nb)
TriMethoxyboroxine
2-Chloro-4-nitrobenzoyl chloride, 98%
3-CHLORO-4,5-DIAMINOBENZOTRIFLUORIDE
1-(Bis-dimethylamino-methylene)-5-chloro-3-oxy-1H-benzotriazol-1-ium hexafluorophosphate
4-(4-Chlorophenyl)-1,2,3,6-tetrahydropyridine hydrochloride, 97%
2,6-Di-tert-butyl-4-hydroxymethylphenol
2,4,6-Trifluorobenzyl bromide 97%
1,1,1-TRIFLUORO-2-BUTANONE
2-Chloro-N-[4-chloro-2-(2-chlorobenzoyl)phenyl]acetamide
DYSPROSIUM
2,4-Difluorobenzaldehyde
1,3-Cyclohexanedione
Dansyl-L-phenylalanine
6-BroMo-2-Mercaptobenzothiazole
PiperonylaMine, 97%
Evans Blue
Methyl Anthranilate
acridin-9-ylamine hydrochloride
1-Chloro-3,3-dimethylbutane
Arbutin
Iodoacetic acid
APATITE
3,5-DI-O-(P-TOLUYL)-2-DEOXY-D-RIBOFURANOSYL CHLORIDE
BariuM Hydroxide Octahydrate
PotassiuM hydrotris(1-pyrazolyl)borate
4-(2-Methylphenyl)-3-thioseMicarbazide, 98%
(2-Hydroxypropyl)-β-cyclodextrin
3,3′-DiMethylnaphthidine
4',7-DiMethoxyisoflavone
α-(TrifluoroMethyl)benzyl alcohol
2-Chloro-5-isocyanatopyridine
DECANOIC ANHYDRIDE
3,5-Di-tert-butyl-o-benzoquinone, 97%
2,4-Dihydroxyphenacyl bromide
Bromobenzene-d5, 99.5 atom% D, for NMR
2-(Bromomethyl)-4-fluorobenzeneboronic acid, neopentyl glycol ester 95%
DL-Ornithine hydrochloride
DIBENZOSUBEROL
TRANS-2-DECENOIC ACID
1-Ethynyl-1-cyclohexanol
3-Fluoro-4-nitrobenzoic acid
Pentachlorophenol
3,4,5,6-TetrachlorophthaliMide
2,2-DiMethyl-5-phenyl-1,3-dioxane-4,6-dione, 98%
1-Benzyl-2-(chloroMethyl)-1H-iMidazole hydrochloride, 97%
DL-THREITOL
9,10-DiMethylanthracene
Flusilazole
5-Chloro-2-hydroxy-4-methylbenzaldehyde
1,3-Dichloro-2,4-difluorobenzene
Prazosin
1-Iodo-3,5-dimethylbenzene
3-Chloro-2,6-difluorophenylboronic acid
TRIETHYLPHOSPHINE OXIDE
N-Methoxy-N-MethyltrifluoroacetaMide
Cyclopentanecarboxaldehyde ,96% [stabilized]
N-(2,4-Dinitrophenyl)glycine
4-Aminobenzo-18-crown-6 sesquihydrate hydrochloride, 99%
methyl 3-hydroxy-1-piperidinecarboxylate(SALTDATA: FREE)
3,5-BIS(TRIFLUOROMETHYL)IODOBENZENE
1H-Tetrazole-1-acetic acid
2-CHLORO-3,6-DIFLUOROPHENOL 97
DiMethyl M-phthalate;Methyl isophthalate
Ethyl Cyclobutanecarboxylate
(-)-AMbroxide
3-Benzyloxy-2-chloro-6-fluorophenylboronic acid
4-Isopropylbenzyl alcohol, 97%
2-BroMo-6-chloro-4-fluoroaniline, 98%
Diisopropyl D-tartrate
7-AMino-4-MethylcouMarin [for HPLC Labeling]
ethyl 5-fluoro-2-nitrobenzoate
4-Benzyloxy-2-fluorobenzeneboronic acid
5-Bromo-2-hydroxy-3-(trifluoromethyl)pyridine
2-Amino-6-methoxybenzoic acid
tPA ELISA Kit
FOXO3 ELISA Kit
Human Histocompatibility 2-K1-K region，H2-K1 ELISA Kit
Human BMPRⅡ ELISA Kit
Col III ELISA Kit
FGF6 ELISA Kit
AGTR1 ELISA Kit
Human CYSLTR2 ELISA Kit
Human CVL/EZR (Cytovillin/Ezrin) ELISA Kit
PANCA ELISA Kit
Nutrient Broth
Tinsdale Agar Base additive
Histamine
SulfaMethazine sodiuM salt
5-BroMo-2-Chloroisonicotinic Acid
2-Hydroxypyridine-4-carboxylic acid, 98%
acridin-9-ylamine hydrochloride
N-Hydroxy succinimide
3,7-Dimethyl-2,6-octadienenitrile, mixtureof isomers
Methyl vinyl ketone
N-Cyanoimido-S,S-dimethyl-dithiocarbonate
2-Piperidone
GadoliniuM(III) chloride, ultra dry, 99.99% (REO)
2-Pentadecanone, 97%
4-Benzyloxybenzotrifluoride, 95%
5-BroMoisatoic anhydride
(1S,2S)-(+)-2-BenzyloxycyclohexylaMine
4-Benzyloxy-3-chloroaniline
Methyl 2,2-difluoro-2-(fluorosulfonyl)acetate
Cholesterol Hydrogen Phthalate , 97%
Basic Green 1
Chromium(III) trifluoride, 99%
4'-Tetrahydropyranylglycine
4-(5-Methyl-1,3,4-oxadiazol-2-yl)benzylamine, 97%
12-Hydroxy-1,2-dihydrosenecionan-11,16-dione
