TU 686细胞：人喉癌复苏快细胞系 货号：GD-C4720574
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Benzoic anhydride, 98%
4,5-Dichloro-o-phenylenediaMine
(1R,3R)-5-(BROMOMETHYLENE)-1,3-BIS(TERT-BUTYLDIMETHYLSILYLOXY)CYCLOHEXANE
METHYL 2-ACETAMIDOBENZOATE
5-Fluoroindole-2-carboxylic acid
YTTRIUM ALUMINUM OXIDE
4-Hydrazinobenzene-1-sulfonaMide hydrochloride
Dodecanedioyl dichloride, 98%
Ethyl 4-(4,4-dimethylpiperidino)benzenecarboxylate
9,10-Dimethylanthracene, 97%
Aceanthrenequinone, 95%
4-Hydroxy-2-methoxybenzaldehyde
Undecanoyl chloride
1-(4-AMINOPHENYL)-4-(4-METHOXYPHENYL)
2-CHLORO-5-METHYLBENZOIC ACID
1-Pentanesulfonic acid
S-(N,N-DiMethylthiocarbaMoyl)thioglycolic Acid
3-BroMopyridazine
SodiuM hydride, 60% dispersion in Mineral oil, in soluble bags
Mepivacaine Hydrochloride, USP
Glycyl-DL-valine
Propiolic acid
4-Pyrimidinamine,6-chloro-5-nitro-
hept-1-yne
N,N,N',N'-Tetramethylethylenediamine
Amylamine
Di-n-butyl ether
Proflavine HeMisulfate
TetraphenylphosphoniuM iodide, 98+%
Triphenylphosphine hydrobroMide, 97%
1,3,5-Benzenetricarbonyl trichloride
(±)-Epichlorohydrin
AMMoniuM Molybdate tetrahydrate
TERT-BUTYL 4-(5-IODOPYRID-2-YL)-1-CARBOXYLATE
2-Mercapto-5-Methyl-1,3,4-thiadiazole
7-HYDROXYCOUMARIN-4-ACETIC ACID
2-Ethyl-1,3-hexanediol (mixture of diastereoisomers)
6-Chloropyridine-2-sulphonic acid
5-Methyl-2-phenyl-2-hexenal
1-amino-6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamide(SALTDATA: FREE)
(S)-ALPHA-METHYL-4-NITROBENZYLAMINE HYDROCHLORIDE
6-CYANONICOTINIC ACID
5,6-Difluoro-2-oxoindole
KANAMYCIN SULFATE
Methyl pyridine-2-carboxylate
N-Acetyl-L-tyrosine Ethyl Ester Monohydrate
1-(3-Nitrophenyl)pyrrolidin-2-one
3,5-DiMethoxybenzyl alcohol, 98%
5-(2-Furyl)-1H-pyrazole-3-carboxylic acid, 97%
TRIHEPTADECANOIN
(R)-4-(4-Methoxybenzyloxy)-1,2-butanediol
1-Aminonaphtalene-5-sulfonic acid
Ceftibuten dihydrate
methyl 3-bromopyridine-4-carboxylate
(6-Chloro-2-pyridinyl)methanol
(4-Pyridylthio)acetic acid
ALPHA-METHYL-GAMMA-BUTYROLACTONE
Graphite
2-AMino-4-ethoxycarbonylphenylboronic acid, HCl
L-Prolinol ,98%
7-Methoxy-3-phenylsulfonyl-1(3H)-isobenzofuranone
Chlorodifluoroacetyl chloride
Direct Yellow 44
D-Xylose
ETHYL ALLOPHANATE
ALPHA-PHENYLCYCLOPENTANEACETIC ACID
RHENIUM (VI) OXIDE
2-AMino-3-iodopyridine
1-(3-AcetaMidophenyl)-5-Mercaptotetrazole
2,4-Dichloro-3-Methylpyridine
4-(4-Nitrophenylazo)resorcinol, 90%
1H-BenziMidazol-5-ylMethanol, 95%
alpha-D-Methylglucoside
eta-Benzene(eta-cyclopentadienyl)iron(II) Hexafluorophosphate
1-Bromotetradecane
(S)-2-(Boc-amino)-2-phenylethylamine
2-Oxa-1,4-butanediol diacetate
(Z)-Dodec-5-enol
Porcine nitric oxide,NO ELISA Kit
IL-21 ELISA Kit
Human TNF-β ELISA Kit
Human TG ELISA Kit
GAL14 ELISA Kit
CD34 ELISA Kit
Monkey IL-12 (Interleukin 12) ELISA Kit
Human Opacity-associated proteins,OPAs ELISA Kit
Human atrial natriuretic factor，ANF ELISA Kit
NT-2R ELISA Kit
Shigella Broth Base
Bacillus Medium Base
1,1,1-TriMethoxypropane
SodiuM Persulfate
5-Hexynoic acid, 97%
5-BroMo-2-(Methylthio)PyriMidine-4-Carboxylic Acid ,97%
Propiolic acid
Zinc nitrate
1,3-Disilabutane
2,4-Dinitroanisole
FERROUS GLUCONATE DIHYDRATE
3-Mercaptopropionic Acid
9-FluorenylMethyl chloroforMate, 98%
Triethyl citrate, 99%
1,3-Diphenylisobenzofuran
(1E)-1-Methoxy-2-Methyl-1-penten-3-one
2-Pentylfuran
GADOLINIUM BROMIDE
Methyl 6-pyrrolidinonicotinate
DL-Panthenol
Ethyl 2-(Bromomethyl)acrylate (stabilized with HQ)
Tetramethylene-d8 sulfone, 99+ atom% D
Sodium DL-2-Hydroxybutyrate
