HEC-1B细胞：人子宫内膜腺癌复苏快细胞系 货号：GD-C4720523
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Methyl-5-(trifluoroMethoxy)benzoic acid, 97%
Hyaluronic acid sodiuM salt froM rooster coMb
TriMorpholinophosphine oxide
AMberlite XAD-1180
3-Fluoropicolinic acid
4-NITROBENZO-15-CROWN-5
2-(Trifluoromethyl)-imidazo[1,2-a]pyrazine
n-Triacontyldimethylchlorosilanetech grade
(4-Methoxy-pyridin-2-yl)-carbamic acid tert-butyl ester
Thiazolidine
2,4'-Dihydroxydiphenyl Sulfone
Trimethylolpropane trimethacrylate
Calcium nitrite
4,6-Dichloro-5-pyrimidinecarbaldehyde
2-(5-Chloro-3-Methylbenzo[b]thiophen-2-yl)acetic acid
4-ChlorobenzylaMine
Diethyl 2,2'-Thiodiglycolate
4-BroMo-3-(trifluoroMethyl)benzaldehyde
2-FluorobenzylaMine, 96%
Trazodone Hydrochloride, USP
N,N,N'',N''-TetrabutyldiethylenetriaMine
Tricyclo(3.3.1.13,7)decane-1-carboxylic acid, methyl ester
2,4,6,8-Tetrahydroxy-Pyrimido-(5,4D)Pyrimidine
calcium diundec-10-enoate
Rosmarinic acid
2-Amino-4-methoxy-6-methylpyrimidine
Flufenoxuron
3-Iodobenzonitrile
O-Benzylphospho-N-FMoc-L-serine, 95%
N-Benzyl-4-fluorobenzylaMine, 97%
2-NitrobenzenesulfonaMide
N,N-DiMethylbutylaMine
3,5-Difluoro-2-nitrophenylboronic acid
N-Methylaniline
1-ACETOXY-1,3-BUTADIENE
N,N-DiethylsalicylaMide
6-Fluoronicotinaldehyde
2-Chlorobenzoylacetonitrile
D-Glutamine methyl ester hydrochloride
N-Carbobenzoxy-L-isoleucine Dicyclohexylammonium Salt
Lofexidine hydrochloride
5-Azacytidine
Cyclobutanecarbonyl chloride
LAURIC ACID SODIUM SALT
2,3-DichlorobenzylaMine
Dibenzo(a,h)anthracene D14 solution
2-Nonene (cis- and trans- Mixture)
IMidocarb
(-)-8-PhenylMenthol, 97%
N-PHENYLAZA-15-CROWN 5-ETHER
1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one
m-Methyl red
1-(methylsulphonyl)imidazolidin-2-one
2-Chloro-3-methylbenzoic acid
o-Sulfobenzoic acid monoammonium salt
DL-2-Octanol
DIMETHYL 2-(THIOPHEN-2-YLMETHYL)MALONATE
Hexyl alcohol
P-TOLUENESULFONYL-N,N-DIETHYLAMIDE
4-Hydroxy-1-methylbicyclo[2.2.2]octan-2-one
4'-Amino-3'-(trifluoromethyl)acetanilide
4-Ethoxy-3-nitropyridine hydrochloride
8-Aminoquinoline N-Oxide
FMOC-D-2-PYRIDYLALANINE
1-CHLORO-3-METHYLBUTANE
1,2,3,4-TETRAHYDRO-9-ACRIDINAMINE
2-Heptanol;MethylaMyl carbinol;sec-Heptyl alcohol;2-Hydroxyheptane;Methyl pentyl carbinol
1,2-Difluoro-4-iodobenzene
1-Ethylpiperidine
Nepafenac
Methyl 2-aMinobenzo[d]thiazole-7-carboxylate
3H-1,2-Benzodithiol-3-one 1,1-dioxide, 98%
Methyl (triphenylphosphoranylidene)acetate
Ethyl Nonanoate
2-Bromo-3-methyl-5-nitropyridine
dichloroisocyanuric acid sodium salt dihydrate
Dimethoxymethylvinylsilane
Trimethylsilyl cyanide
GDF1 ELISA Kit
CACNα1D ELISA Kit
Human Helicobacter pylori IgG ，Hp-IgG ELISA Kit
Human MRP1 (Multidrug Resistance Associated Protein 1) ELISA Kit
Monkey apoprotein B100,apo-B100 ELISA Kit
γSAP ELISA Kit
CA2 ELISA Kit
Human PKCβ1 ELISA Kit
PAF ELISA Kit
FABP ELISA Kit
Dulbecco’s Phosphate Buffered Saline(D-PBS)
Cary-Blair Transport Medium
Trichlorovinylsilane
Furan-2-thiocarboxaMide, 97%
5-(2-CHLOROPHENYL)-2-FUROIC ACID
2-Iodobenzoic acid, 98+%
Tricyclo(3.3.1.13,7)decane-1-carboxylic acid, methyl ester
Benzo-18-crown-6
Acid Yellow 116
Tert-butyl nitrite
2,5-Dimethylphenylacetonitrile
4-Chloro-2-fluorophenol
Diisobutyl adipate
AMMoniuM diaMMinetetrathiocyanatochroMate(III) Monohydrate, ACS, 93.0% Min
(+)-Fenchone, 98%
Tetrahydrofuran-3-ylaMine hydrochloride
Tributylphosphine solution
Methyl 1H-indazole-6-carboxylate
(S)-1-[(RP)-2-(Diphenylphosphino)ferrocenyl]ethyldi(3,5-xylyl)phosphine
3-(2-Naphthyl)-D-alanine
1,2-Dichloropropane
2,3-Dichlorophenyl isocyanate
(4-sec-butylphenyl)boronic acid(SALTDATA: FREE)
Ethyl 2-(Hydroxymethyl)acrylate (stabilized with HQ)
5-Nitro-2-(3-piperidinylmethoxy)pyridine hydrochloride
