NCI-H295R[H295R]细胞：人肾上腺皮质腺癌复苏快细胞系

NCI-H295R[H295R]细胞：人肾上腺皮质腺癌复苏快细胞系 货号：GD-C4720366
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3'-Chloroacetophenone, 98+%
(R)-(-)-1-Methyl-3-pyrrolidinol
SodiuM acetate-1-13C
2-HYDROXY-4-METHYLQUINOLINE
Fluoxetine hydrochloride
8-Anilino-1-naphthalenesulfonic acid MagnesiuM salt
Lauryl chloroforMate
(3-Piperidinophenyl)methylamine , 95%
2-(1,3-Benzodioxol-5-yl)-6-chloroimidazo[1,2-b]pyridazine
4,5,6,7-Tetrahydro-3-(trifluoromethyl)-1H-indazole
4-Benzoyl benzylamine hydrochloride
4-Pyridineethanol
3,5-BIS(METHYLSULFONYL)ANILINE
alpha,alpha,alpha-Trifluoro-o-toluoyl chloride
2-Cyano-5-Methylbenzenesulfonyl chloride
FlufenaMic acid
2,4-DiMethyloxazole
N-Methyl 4-broMobenzenesulfonaMide
VancoMycin hydrochloride, >900 MicrograM/Mg
SodiuM Pyrophosphate, Decahydrate, Crystal, Reagent, ACS
Hydroquinidine Hydrochloride
Ethyl Azidoacetate
Brilliant Blue G
8-Fluoroisoquinoline
Alpha,Alpha,Alpha-Trifluoro-p-tolunitrile
Decanoyl chloride
3-Bromobenzenesulfonyl chloride
4-Fluoro-3-Hydroxybenzonitrile
Dicarbonylcyclopentadienylcobalt
AdaMantane-1,3-dicarboxylic acid, 98%
3-AMinobenzenesulfonaMide, tech. 80%
4-PiperidineMethanol
1,1,2,3,3-PENTACHLOROPROPANE
PENTACHLOROTHIOPHENOL
2,4-DIMETHYL-6-NITROPHENOL
1-BroMo-4-(MethoxyMethoxy)benzene
(R)-(+)-(3-Chlorophenyl)oxirane
Ethyldiphenylphosphine, 99%
2,2'-Dihydroxydiphenylmethane
(+/-)-Limonene
(Chloromethyl)dimethylvinylsilane(Vinyl(chloromethyl)dimethylsilane)
Tetraphenylmethane
N-Methyl-L-prolinol
3-FLUORO-L-PHENYLALANINE
PotassiuM pyrosulfate
Benzanilide
3,5-Dichloro-2,4-difluoroaniline
Undecafluorohexanoyl Fluoride
Tris(4-broMophenyl)aMiniuM hexachloroantiMonate, 95%
SeleniuM Sulfide, USP
Diisodecyl 4-Cyclohexene-1,2-dicarboxylate
N-propylaniline
Pigment Red 2
ethambutol dihydrochloride
2-Fluoronicotinic acid
TETRAPROPYLAMMONIUM PERCHLORATE
sec-ButylaMine
5-BroMo-2-benzoxazolinone
2-Methylfuran-3-boronic acid, pinacol ester
5-Fluorouracil
3-Iodoisopropylbenzene
4-Chloro-2,3-dimethylpyridine-N-oxide ,98%
Methyl 4'-nitro[1,1'-biphenyl]-4-carboxylate
2-(4-NITROPHENOXY)ETHANOL
2-Norbornanemethanol
3-CHLORO-2-FLUORONITROBENZENE
CalciuM chloride fused
DI-TERT-BUTYL PHOSPHITE
1-BroMo-2,3-dichlorobenzene
4-MethoxycinnaMonitrile (cis- and trans- Mixture)
Tetrahydrothiophene-3-carboxylic acid 1,1-dioxide
2,6-Difluorobenzaldehyde, 98%
Tungstic acid
3-Pyridylacetic Acid Hydrochloride
ethylenediaminetetraacetic acid*trisodium
Glutaraldehyde sodium bisulfite addition compound
Dextrin
1-chloro-3-ethoxy-2-propanol
PPIL1 ELISA Kit
Smad7 ELISA Kit
Human Progesterone，PROG ELISA Kit
E2/UBCE ELISA Kit
IL13 ELISA Kit
A-GST ELISA Kit
Chicken Myoglobin,MYO/MB ELISA Kit
CH ELISA Kit
Human hemoglobin-advanced glycosylation end products,Hb-AGE ELISA Kit
TAP ELISA Kit
Modified Plate Count Agar
Letheen Broth Base
Ethoxyamine hydrochloride
FluoroMethyl 1,1,1,3,3,3-hexafluoroisopropyl ether, 98+%
5,7-Dichloro-4-Hydroxy-2-(TrifluoroMethyl)Quinoline
2-Fluoro-4-nitrobenzoic acid, 98%
Ethyl Azidoacetate
4-Aminoanisole-3-sulfonic acid
4-nitrophenyl-alpha-L-fucopyranoside
3,5-Dimethylbenzoic acid

SODIUM 4-N-OCTYLBENZENESULFONATE
N-(ISOBUTOXYMETHYL)ACRYLAMIDE
3-Hydroxy-5-Methylisoxazole, 97%
2-(4-Methylphenyl)-4-phenylthiazole, 97%
SodiuM Metasilicate, anhydrous, tech.
tert-Butyl isocyanate
6-Methylnicotinic Acid Methyl Ester
N-Methoxy-N-Methyl 4-borono-2-fluorobenzaMide
2-Chloro-6-trifluoroMethylnicotinic acid
3-CHLORO-4-FLUOROPHENYL ISOCYANATE
1-(4-METHOXY-PHENYL)-2-METHYLAMINO-ETHANOL
Benzyl-propyl-amine
Lead citrate, trihydrate, 96%
7-Methylindole-3-carboxaldehyde 98%
1,2,3,4-Tetrahydrocarbazol-4-one