HEC-1-B细胞：人子宫内膜腺癌复苏快细胞系 货号：GD-C4718271
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-Ethylcyclohexanecarboxylic acid, cis + trans, 97%
Ethyl 4-nitrobenzoate
Sulfadiazine, 99%
2,5-DibroMo-1-trityliMidazole
3-IsobutyraMidophenylboronic acid
TETRAETHYLSILANE
2-Methyl-1,4-bis(triMethylsiloxy)benzene, 98%
5-amino-4-isoxazolecarbonitrile(SALTDATA: FREE)
Sodium sulfide nonahydrate, 98+% (ACS)
2-Bromo-1-(5-chloro-2-hydroxyphenyl)ethanone
2-(Trifluoromethyl)anisole
1-Chloroethyl ethyl carbonate
IODOCYCLOPENTANE
2,4,6-TRINITRORESORCINOL
5-Bromo-3-nitro-2-pyridinol
Adenosine 3',5'-cyclic Monophosphate
2,3-Dihydro-2-Methylbenzofuran
5-BroMo-2-Methoxybenzoic acid
PlatinuM(IV) oxide, 83% Pt
FD and C Red No. 40
MoMetasone Furoate
Methyclothiazide
Fluorescent brightener 185
furan-3-carboxaldehyde
2-Ethyl-2-(hydroxymethyl)-1,3-propanediol
BREDERECK'S REAGENT
MERCURIC PERCHLORATE
2-FluorocinnaMaldehyde
trans-3,5-DifluorocinnaMic acid, 99%
1,2,4-Benzenetricarboxylic acid, 98%
N-(Benzyloxycarbonyloxy)succiniMide
2-(4-Methoxyphenyl)
ChaMoMile oil, Moroccan
ZIRCONIUM(IV) ACETYLACETONATE
2-(4-Acetylpiperazino)-5-broMopyridine
Boron trifluoride-acetic acid coMplex
2-Bromo-4-fluorophenol 97%
9H-Xanthene-9-carboxylic acid
Methyl 2,4-dichlorophenylacetate, 99%
spiro-[3.3]-Heptane-2,6-dicarboxylic acid fecht's acid
3,4-DIHYDRO-2H-PYRAN-5-CARBALDEHYDE
N-(N-(N-Glycylglycyl)glycyl)-8-L-lysinevasopressin
N-BOC-4-Hydroxypiperidine
MALT EXTRACT
Benzyl 2,2,2-trichloroacetiMidate
Cvesol red
alpha-Methyl-gaMMa-butyrolactone
N-tert-Butyl-2-chlorobenzaMide
Nitrobenzo-18-crown-6, 99+%
Ethyl 3-aminopropanoate hydrochloride
5-tert-Butyl-M-xylene
DL-Phenylsuccinic acid
(R)-(-)-O-formylmandeloyl chloride
glucagon
benzo[1,2-c:4,5-c']diacridine-6,9,15,18(5H,14H)-tetrone
Gadolinium oxide
4,4',4''-TRICHLOROTRITYL ALCOHOL
(S)-(-)-N-Benzyl-1-phenylethylaMine
Tangeretin
Isonicotinic acid ,99%
Carbonic Anhydrase [from bovine erythrocytes, 2000 units/mg?]
3-(Trifluoromethyl)phenylacetone
1-pyridin-3-ylacetone(SALTDATA: HCl)
5-Bromo-4-chloro-3-indolyl-beta-D-glucoside
Butyl thiocyanate
7-CHLORO-1H-INDAZOLE-3-CARBOXYLIC ACID
CACOTHELINE
1,3-Dichloro-2-iodo-5-(trifluoroMethyl)benzene
(-)-Dihydrocarvyl acetate
Methyl 3-Methoxysalicylate
H-P-Fluoro-phe-oet hcl
2-Fluoro-5-forMylbenzonitrile, 99%
4-Bromothioanisole
4-(3-Phenylpropyl)pyridine
2-Methylthiopyridine
Valienamine
Phenmedipham
terbuthylazine
Cath-S ELISA Kit
LN ELISA Kit
Human Tissue Polypeptide Antigen,TPA ELISA Kit
BMP7 ELISA Kit
HSP90αB1 ELISA Kit
Tie2 ELISA Kit
ATA ELISA Kit
Human GAL2 (Galectin 2 ) ELISA Kit
Human lipocalin 2,LCN2 ELISA Kit
sCD163 ELISA Kit
Tryptic Soy Agar
Murashige & Skoog Macronutrients
2-ETHYLHEXYL PALMITATE
SulfanilaMide, 98%
5-BroMo-2-chlorobenzonitrile
2-HydroxypyriMidine-5-boronic acid pinacol ester, 95%
Methyclothiazide
1-Methyl-1,2,4-triazole
Lakered CBA
Bromoacetic acid
2,2,3,3,3-PENTAFLUOROPROPYLAMINE
(S)-N-(Benzyloxycarbonyl)Asparagine
6-Methoxy-2-Methylquinoline, 97%
Diphenylphosphonic azide, 97%
2-Hexyn-1-ol, 97%
YttriuM
Methyl (S)-PyroglutaMate
1,1-Diiodo-2,2-diMethylpropane
2-Chloro-5-iodotoluene
4,4′-DiMercaptostilbene
2-(DibutylaMino)ethanol
N-Methyl-3-[(6-methylpyrazin-2-yl)oxy]benzylamine , 97%
4-(2-Piperazin-1-yl-ethyl)-morpholine
Ethylenediamine-N,N'-dipropionic Acid Dihydrochloride
Potassium (4-methylsulphonylphenyl)trifluoroborate
