MC-3T3细胞：小鼠前成骨复苏快细胞系 货号：GD-C4719684
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
L-Proline t-butyl ester
2-Chloro-3,4-diMethoxybenzaldehyde
15-Crown-4 [4-(2,4-Dinitrophenylazo)phenol] , 98%
4-DiethylaMinobenzaldehyde
1,1,2,2,3,3-HEXACHLOROPROPANE
3-AMino-5-broMo-2-chloropyridine
2-(4-Benzylpiperdin-1-yl)ethylamine
Cefodizime Sodium
Magnesium perchlorate dihydrate, for analysis, 89%
(R)-(+)-2-(Diphenylphosphino)-2'-methoxy-1,1'-binaphthyl, 99% (R)-MOP
2-Bromobutyryl bromide
Ethylene carbonate
1,2-Dibromoethane
CALCIUM HYDRIDE
Tri(o-tolyl)phosphine
Benzidine
1-Ethylpyrrolidine
6-AcetaMido-3-broMopicolinic acid
1-[(tert-Butoxycarbonyl)aMino]cyclohexanecarboxylic acid, 97%
DEMETON-S
O-tert-Butyl-L-serine Methyl Ester Hydrochloride
2-Fluoro-4-nitrotoluene
Reactive Violet 2
ethyl 3-phenylpropionate
4-Nitrobenzonitrile
Thulium trifluoride
4-tert-Butylphenylboronic acid
CaMphene
2-Chloro-5-n-decylpyriMidine, 99%
^a,^a-DiMethylbenzylaMine, 96%
2-Chloro-4-fluoroanisole
Cobalt(II) hydroxide
SodiuM hypochlorite
N,N'-BIS(4-CHLOROPHENYL)-N,N'-DIPHENYL-1,4-PHENYLENEDIAMINE
2-(4-METHOXYPHENYL)ETHANETHIOAMIDE
METHYL 2-NAPHTHOATE
Prostaglandin E2, 98%
Phenyl sulfone, 97%
alpha-Bromo-o-xylene
3-Chlorophenylacetone, 94%
GLYCYLGLYCINE HYDROCHLORIDE
2,2-Dimethylsuccinic acid
CALCIUM CYANIDE
Epichlorohydrin;DL-α-Epichlorohydrin;γ-Chloropropylene oxide;ChloroMethyloxirane
Pyrrolidine-1-carbonitrile
H-β-HoMoglutaMic acid hydrochloride
Propyl 3-Propoxypyridine-2-carboxylate
(R)-(-)-1-[(S)-2-Diphenylphosphino)ferrocenyl]ethyldicyclohexylphosphine, 98%
8-AMino-5-broMoisoquinoline, 97%
H-TYR-NH2 HCL
2,4-Difluorobenzaldehyde
Vinpocetine
Hydrogenated terphenyls
2-Methylbenzyl cyanide
2-Chloro-3-oxopentyl acetate
EUROPIUM CHLORIDE
1,4-DIACETYLBENZENE
BETA-(4-MORPHOLINO)PROPIOPHENONE HYDROCHLORIDE
ETHYL 3-OXO-4-(TRIPHENYLPHOSPHORANYLIDENE)BUTYRATE
SodiuM-potassiuM alloy
Phenylthiohydantoin-valine
1-Benzyl D-Glutamate
1,3-Pyrrolidinedicarboxylic acid 4-oxo-1-(1,1-dimethylethyl) 3-ethyl ester
4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-D-glucopyranoside
2-(Aminomethyl)-1-N-Boc-pyrrolidine
5-(TERT-BUTYL)-2-METHYLFURAN-3-CARBONYL CHLORIDE
[1,2-Bis(diphenyphosphino)ethane]dichloroiron(II)
2-Bromo-3-methylpyridine
2,2-DiMethyl-4-pentenal
(S)-2,2'-DiMethyl-1,1'-binaphthyl
2-(t-ButyldiMethylsilyloxy)-4-broMoanisole
TetraMethylene sulfone, 99+%
N-Methyl-L-Alanine
4,4',4''-Trifluorotrityl BroMide
(3β)-olean-12-ene-3,28-diol
(2S)-[(2S,3R,4R)-3,4-dihydroxy-5-oxotetrahydrofuran-2-yl](hydroxy)ethanoic acid (non-preferred name)
Cyclopropyl methyl carbinol
Butyl Hydrogen Phthalate
VASPIN ELISA Kit
ABCG2 ELISA Kit
IGFBP4 ELISA Kit
Human LDL-IC ELISA Kit
CA724 ELISA Kit
C.albicans ELISA Kit
Equine growth hormone,GH ELISA Kit
Human TERF2 ELISA Kit
Human ISR-β ELISA Kit
HELIX-Ⅱ ELISA Kit
GVPC Liquid Medium
B12 Assay Medium
Ethyl 1-Boc-DL-nipecotate, 97%
trans-2-Undecenal, 95%, reMainder Mainly cis-isoMer
4-Ethylcyclohexene-1-boronic acid, 98%
Anthranilonitrile
2-Fluoro-4-nitrotoluene
3,4-Diaminofurazan
4,5-dibromo-2-phenyl-2,3-dihydropyridazin-3-one
1,2-Dibromotetrachloroethane
N,N,N',N'-Tetramethylethylenediamine
2,2'-BIPHENYLDIMETHANOL
4-AMino-3-BroMo-2-Chloropyridine
1,4-Diacetoxybenzene, 98%
1-MethyliMidazole-5-carboxaldehyde, 97%
Tri-n-butyl(1-ethoxyvinyl)tin
1-Chlorotetradecane
5-BroMo-2-(4-Methoxybenzyloxy)pyridine
Methyl 2-aMino-4-cyclopropylthiazole-5-carboxylate
N-HEXYLLITHIUM
METHYL 3-OXOHEPTANOATE
1-Eth-1-ynyl-4-(pentyloxy)benzene
(2S,4S)-(+)-2,4-Pentanediol, 99%
1-Methyl-2-imidazolidinone ,98%
Ethyl 3-ethyl-5-methylisoxazole-4-carboxylate
