K7M2wt细胞：小鼠骨肉瘤成骨复苏快细胞系

K7M2wt细胞：小鼠骨肉瘤成骨复苏快细胞系 货号：GD-C4719656
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-(4-Hydroxyphenyl)ethanol
N-AcetylethanolaMine
(3R,4R)-3,4-Bis(diphenylphosphino)-1-benzylpyrrolidine
VARIAMINE BLUE B SULFATE (=4-AMINO-4'-METHOXYDIPHENYLAMINE SULFATE)[FOR IRON-TITRATION]
2-BROMO-4-NITRO(TRIFLUOROMETHOXY)BENZENE
4-Hydroxy-1-indanone benzoate
2-(4-Methoxyphenyl)quinoline-4-carboxylic acid
(4-Bromophenyl)methanesulfonyl chloride , 97%
2-(Methylthio)pyrimidine-5-carbonitrile ,97%
N-Myristoyl-D-erythro-sphingosine, synthetical, 99+%
(+)-NEOMENTHOL
3-FLUORO-2-FORMYLPYRIDINE
2,3-Dimethoxy-5-methyl-p-benzoquinone
(+)-1,4-DI-O-TOSYL-2,3-O-ISOPROPYLIDENE-D-THREITOL
3,3,3-Trifluoro-2,2-diMethylpropionic acid
2-CyanopyriMidine
Ethyl (S)-(-)-1-Ethyl-2-pyrrolidinecarboxylate
tert-Butyl trans-4-forMylcyclohexylcarbaMate
2-BroMo-1-[2,6-dichloro-4-(trifluoroMethyl)phenyl]ethan-1-one, 95%
CHLOROACETONITRILE
Copper(I) TrifluoroMethanesulfonate Benzene CoMplex
polymyxin B standard solution
germanium tetrafluoride
Sodium Houttuyfonate
Ethyl 3-nitrobenzoate
Manganese triacetate dihydrate
2-Bromo-5-iodopyridine
2,3-Difluoro-6-Nitroaniline
LanthanuM(III) hydroxide, 99.95% (REO)
N,N,N',N'-TetraMethylMethylenediaMine, 99%
Valeric acid
trans-3-Indoleacrylic acid
DIISOBUTYL PHOSPHITE
N,N-Diethylaniline
SODIUM COBALTINITRITE
HexaMethylene diacrylate
4-Chloro-1-methylpiperidine Hydrochloride
3-Methylamino-1,2-propanediol
4-Chloro-5-nitro-o-phenylenediamine, 98%
N-Methylaniline
Hexahydro-1,3,5-tris(hydroxyethyl)-s-triazine
Pilocarpine nitrate
1-IODO-2,3,4,5-TETRAMETHYLBENZENE
QUINACRINE DIHYDROCHLORIDE
4-BroMo-2-Methylaniline
Bis(1,5-cyclooctadiene)nickel(0)
3,4-MethylenedioxycinnaMic Acid
4-(2-Chloro-4-nitrophenyl)Morpholine
2-Chloro-3-fluoropyridine, 97%
Arabic gum
2-Fluorobenzaldehyde
Basil oil french
2-s-Thiuronium ethanesulfonate
N,N-dicyclohexylmethylamine
5-Acetyl-2,2'-Bithiophene
4-[5-(4,4,5,5-TETRAMETHYL-[1,3,2]DIOXABOROLAN-2-YL)-PYRIDIN-2-YL]--1-CARBOXYLIC ACID TERT-BUTYL ESTER
2-BroMo-1-(difluoroMethyl)-4-fluorobenzene
Bis(diMethylaMino)diMethylsilane
5-CHLOROSALICYLANILIDE
(R)-N-FMOC-4-Fluorophenylalanine, 98% ee, 95%
1-Azido-1-deoxy-β-D-galactopyranoside ,97%
4,5,6,7-Tetrahydro-1H-indole-2-carboxylic acid
Fmoc-L-2-indanylglycine
2,4-BIS(TRIFLUOROMETHYL)BENZALDEHYDE
4-N-BUTYLBENZENESULFONAMIDE
1,6-Diiodododecafluorohexane
2,4,6-TRICHLOROPHENOXYACETIC ACID
2-[(4-Chlorophenyl)sulfonyl]ethanethioaMide
2,2-Bis(4-carboxyphenyl)hexafluoropropane
3-Butoxy-5-Methylphenylboronic acid
alpha,alpha'-DibroMo-p-xylene, 97%
Isobutanesulfonyl chloride, 97%
Ibudilast
N-Benzoyl-DL-phenylalanine 2-Naphthyl Ester [for DeterMination of ChyMotrypsin]
1,3-dimethyl-1,3-diphenylurea
6,7-Dihydro-4H-pyrano[4,3-d][1,3]thiazol-2-amine
N-T-boc-ala-pro-nva P-chlorothiobenzyl*ester
N,1-dimethylpyrrolidin-3-amine
TAT ELISA Kit
IκBKβ ELISA Kit
Human IA-2A ELISA Kit
Human hypoxia-inducible factor 1α，HIF-1α ELISA Kit
CDKN2A ELISA Kit
IL－8 ELISA Kit
MMP1 ELISA Kit
Human CHRM4 ELISA Kit
Human Try2 (Trypsinogen II) ELISA Kit
NTXⅠ ELISA Kit
Azospirillum Medium
Martin Agar
Ethyl 3-oxo-3-(4-pyridyl)propionate, 98%
trans-4-(4-n-Pentylcyclohexyl)benzonitrile, 97%
4-Vinylbiphenyl, 97%
2-AMinopyridine-4-carboxylic acid, 96%
polymyxin B standard solution
2,3-DICHLOROBIPHENYL
(S)-7-[[6-O-(6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl]oxy]-2,3-dihydro-5-hydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one
N,N'-Diphenylethanediamide

Isonicotinic acid
N-BENZYLOXYCARBONYL-D-ALANINOL
Lovastatin
2-AMinoadenosine, 97%
Bis(cyclopentadienyl)vanadiuM broMide, 98%
Bis(2-oxo-3-oxazolidinyl)phosphonic chloride
TryptaMine hydrochloride
Chlorfenazole
Phenylacetyl chloride
Reference Material of Hydrocarbon Types in Gasoline by FIA Certified by The Japan PetroleuM Institute, Olefins Content 5vol% level (5Ml*3)
AMbersep 900(OH), ion exchange resin
3,5-Dimethyl-1-phenyl-1H-pyrazole-4-carboxylic acid , 97%
[1,3]Oxazolo[4,5-b]pyridin-2-ol , 97%
Dichlorobis[3-(di-t-butylphosphino)propylamine]ruthenium(II), min. 97%
(S,S)-2,6-Bis(4-isopropyl-2-oxazolin-2-yl)pyridine