Bv-2细胞：小鼠小胶质瘤贴壁细胞系

Bv-2细胞：小鼠小胶质瘤贴壁细胞系 货号：GD-C4720505
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
AluMinuM oxide, catalyst support, interMediate surface area (low SiO{2})
DL-Lactic acid
3,5-DIMETHYLTHIOPHENOL
2-Chloro-4-broMobenzaldehyde
Ethyl 1-Boc-piperidine-2-carboxylate
N-CinnaMoyl-N-(2,3-xylyl)hydroxylaMine , 98%
5-Cyano-1H-indole-2-boronic acid, N-BOC protected 95%
2-Oxindole-5-carboxylic acid 97%
Methyl 5-bromo-2-methoxynicotinate
Dodecylbenzene (hard type) (mixture of branched chain isomers)
Tri(4-morpholino)phosphine oxide ,99%
FERROIN
2-AMINO-N-METHYLBENZAMIDE
2-Butoxyethyl acetate
1-Iodo-4-nitrobenzene
4-Chlorophenylboronic Acid
Di-tert-butyl IMinodiacetate
Methyl 2-broMo-3,3,3-trifluoropropionate
4-Iodo-M-xylene, 95%
TiMolol Maleate, USP
Dienestrol
γ-Decalactone
1,2-dibromo-4-tert-butylbenzene
Calcium carbonate
11-octadecenoic acid
Pentafluorophenol
4,4'-Diaminobenzophenone
4-Fluoro-N-MethylbenzylaMine
1,2-Bis(1-naphthyl)ethane, 97%
2-Fluoro-N,N-diisobutylbenzaMide, 97%
Clodinafop-propargyl
Cyclopentanecarbonitrile
Dihydrogen hexachloroiridate(IV) hydrate, 99% (Metals basis), Ir 38-42%
N-NONADECYLBENZENE
N-METHYL-P-TOLUIDINE
3-AMino-9-fluorenone
5-Bromoindoline, 97%
Ethyl 2-cyano-3-methyl-2-butenoate, 97%
4-Methoxy-3-methylaniline
Chromium(III) 2-ethylhexanoate, 70% in mineral spirits (8-10% Cr)
4-CYANOPHENYL 4-N-BUTYLBENZOATE
Fucoidan
2'-(TRIFLUOROMETHOXY)ACETOPHENONE
Fmoc-O-tert-butyl-L-tyrosine
(R)-(+)-1-(4-Methoxyphenyl)ethylaMine
DexaMethasone 21-acetate
4-Methoxyphenyl 4-(3-Butenyloxy)benzoate
1-(4-Fluorophenyl)cyclopropylaMine
5-Methoxyindole-3-carboxaldehyde, 99+%
CARBETAPENTANE CITRATE
4-Isopropyl-3-Methylphenol
Dialuminum trisulfate hydrate
5-bromo-3-chloro-2-fluoropyridine
CALCIUM SULFATE HEMIHYDRATE
5-Chloro-2-pentanone
2,6-Dichlorobenzamide
3-TRIMETHYLSILYL-2-OXAZOLIDINONE
LithiuM Metagallate, 99%
Borane-Methyl sulfide coMplex
Diethyl [(ethoxycarbonyl)fluoromethyl]phosphonate 96%
2-Iodobenzamide
2,2-Di(4,4-di(tert-butylperoxy)cyclohexyl)propane, 20 wt% solution in ethylbenzene
(R)-N-(2,3-Dihydroxypropoxy)-3,4-difluoro-2-(2-fluoro-4-iodophenylamino)benzamide
4-Amino-5-chloro-2,1,3-benzothiadiazole
1-Decyne
6-NITROCOUMARIN
5-Methyl-2-hexanone
2-Mercaptopropionic acid
1-Decanol
4-tert-Butylcalix[6]arene (contains 5-10% Benzene)
1-Fluoro-2,4-diMethoxybenzene
2,2-DibroMopropane, 98%
Anisonitrile
Trichloroacetic Anhydride
Ethyl morpholinoacetate
4-AMINO-NICOTINONITRILE
2-Bromomethyl-1,3-dioxolane
S-Methyl thioacetate
GH2 ELISA Kit
NANOG ELISA Kit
Human Free Thyroxine,FT4 ELISA Kit
Human OTOL1 ELISA Kit
IL-8/CXCL8 ELISA Kit
Rat β-Thromboglobulin,β-TG ELISA Kit
Chicken adrenomedullin,ADM ELISA Kit
Human PKBβ ELISA Kit
Human thrombospondin 1,TSP-1 ELISA Kit
CENPE ELISA Kit
Azide Blood Agar Base
Bile Esculin Azide Agar
Acetyl chloride
Furazolidone
5-(2-Thienyl)pentanoic acid, 95%
Nonafluoro-2-iodobutane, 98%, stab. with copper
γ-Decalactone
l-Menthone
Solvent Orange 54
isobutyl butyrate

4-CUMYLPHENOL
Hesperidin
6-Methyl-5-Hepten-2-One
6-Chloropyridine-3-Methanol, 98%
Uracil, 99+%
Maltol Propionate
2-Ethylaniline
4-(AMinoMethyl)tetrahydro-2H-pyran
2-ACETYLCYCLOHEXANONE
(S)-1-{(RP)-2-[Bis[3,5-bis(trifluoroMethyl)phenyl]phosphino]-ferrocenyl}ethyldicyclohexylphosphine
3-Cyano-1-propylboronic acid pinacol ester, 96%
1-Iodo-4-isopropylbenzene
2,6-Difluoro-3-methylbenzyl bromide
2-Methyl-thiazole-4-carboxylic acid hydrazide
4-Hydroxy-3-isothiocyanatobutyric acid lactone