SGC-790细胞：人胃癌传代细胞系 货号：GD-C4620558
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2',5'-Dihydroxypropiophenone, 97%
Agar
3-(EthylaMino)propionitrile
4-Chloro-3-(trifluoroMethoxy)benzonitrile, 97%
N-PROPYLDICHLOROPHOSPHINE
cis-Bis(triphenylphosphine)platinuM(II) chloride
2-AMinobenzenearsonic acid
4'-Carboxybenzo-15-crown 5-Ether
4-Fluoro-2-methylphenol
Diphenyl chloridophosphate ,99%
3,5-Dibromo-2-hydroxypyrazine
2-Ethyl-1-hexanethiol
4-METHOXYCINNAMIC ACID 2-ETHYLHEXYL ESTER
CHLOROMESITYLENE
2,2,2-Trifluoroethyl trifluoromethanesulfonate
cis-DichlorodiaMineplatinuM(II)
Dodecafluorosuberic Acid
5-BroMo-4-propylpyriMidine
Squalane, 99%
Mastic GuM, Tears
1,3,3-TriMethylindolino-8'-Methoxybenzopyrylospiran [PhotochroMic CoMpound]
20-ethyl-6,16-dimethoxy-4-(methoxymethyl)aconitane-1,8,14-tr...
4-ethynyl-1,2-dimethoxybenzene
hexanamide
3-Pyrroline
4-(Trifluoromethoxy)benzyl bromide
5-FLUORO-2-METHOXYBENZONITRILE
3-Methylindole
2-AdaMantylzinc broMide, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
N-tert-Butyl-2-chloropyridine-4-carboxaMide, 95%
Ethyl benzoylacetate
Bis(3,5-diMethylphenyl)phosphine oxide
1-PROPYN-1-YL IODIDE
tert-Butylboronic acid pinacol ester
Phytic acid
3,4,5-Trifluorobenzoyl chloride
2-Nitro-4-(trifluoromethyl)benzoic acid
2-(4-Methoxybenzylidene)succinic acid , 97%
2,4,6,8-Tetrachloropyrimido[5,4-d]pyrimidine
5-Bromo-1,2-benzisoxazol-3-ylamine, 97%
Boc-D-Phenylglycine
2,5-DICHLOROPHENYLHYDRAZINE
2-Bromophenol
HexaMethyldisiloxane;HMDSO
PalladiuM(II) 2,4-pentanedionate
Isobutyl acetoacetate
Ethyl UndecafluoroaMyl Ketone
(S)-(+)-5-HydroxyMethyl-2-pyrrolidinone, 97%
5-BroMo-1H-indazole, 97%
PTH-VALINE
Direct Blue 6
2-Octanol, (2R)-
Silicone oil
2-Amino-3,5-diiodopyridine
cadmium bromide tetrahydrate
1-CHLORO-4-ETHYLBENZENE
CHROMIUM (IC) PHOSPHATE
1-ETHYLHOMO
2,6-DINITRO-4-FLUOROPHENOL
4-Methylmorpholine N-oxide monohydrate ,97%
1-Phenyl-1-hexanol
2,5-Di-tert-butyl-1,4-benzoquinone
Dimethyldiphenylphosphonium iodide, 98%
2-Chloro-4-fluorobenzoyl chloride
ETHYL (R)-(-)-MANDELATE
Furfuryl thiopropionate
2,3,4-Trimethoxybenzoic acid
3,5-Bis(trifluoroMethyl)phenylacetic Acid
1-(2-Cyanoethyl)-2-ethyl-4-MethyliMidazole (contains 5-Methyl isoMer)
2-Piperazinopyridine-5-boronic acid
HexaMethyldisiloxane, 98+%
4-Pyridylacetonitrile Monohydrochloride, 98%
(S)-1-(tert-Butoxycarbonyl)-2-piperidinecarboxylic Acid
Valeric Anhydride
2-Amino-6-methylbenzothiazole
2,5-Dichloroaniline
2-(Aminomethyl)-1-N-Boc-pyrrolidine
o-(Methacryloxyethyl)-N-(triethoxysilylpropyl)urethane
IDE ELISA Kit
Mouse IL-25 (Interleukin 25) ELISA Kit
Human TNFSF14 ELISA Kit
EFNA1 ELISA Kit
αFP ELISA Kit
CD14 ELISA Kit
Monkey AMBP (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
CFP ELISA Kit
CT1 ELISA Kit
GFAP ELISA Kit
Salmonella Shigella Agar
Semi-solid Medium
CesiuM orthovanadate, 99.9% (Metals basis)
Benzoyl Peroxide
5-Methyl-1-hexyn-3-ol, 97%
2-Methyltetrahydrofuran, 99%, stab. with ca 150-400ppM BHT
20-ethyl-6,16-dimethoxy-4-(methoxymethyl)aconitane-1,8,14-tr...
sulfamyl chloride
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt
2,4,6-Tribromophenol
2,6-Difluorobenzyl chloride
(S)-1-[(1R)-2-(DIPHENYLPHOSPHINO)FERROCENYL]ETHYLDI-TERT-BUTYLPHOSPHINE
Boc-D-PyroglutaMinol
(R)-N-Boc-2-hydroxyMethylMorpholine, 99%
1,4-Benzenediboronic acid, 96%
FenhexaMid
[10]-Gingerol
4-BroMo-6-chloro-2-(trifluoroMethyl)quinoline
4-Pyridineethanol
2-Chloro-6-fluorophenylboronic acid
4-Fluorostyrene
N-(tert-Butoxycarbonyl)-O-benzyl-L-threonine
1-Aminocyclopropanecarboxylic acid hydrochloride, 97%
4,5,6,7-tetrahydro-2,1-benzisoxazole-3-carboxylic acid(SALTDATA: FREE)
3-[3,5-Di(tert-butyl)-4-hydroxyphenyl]-propanoic acid
