OCM-1A细胞：人低侵袭性脉络膜黑色素素瘤传代细胞系 货号：GD-C4619045
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
5-AMino-3-(2-thienyl)-1H-pyrazole, 98%
2-AMino-5-(trifluoroMethyl)pyridine
2,6-Bis[(4R,5R)-4-Methyl-5-phenyl-2-oxazolinyl]pyridine
2-Fluoro-3-trifluoroMethylphenylboronic acid
3-METHYLOXINDOLE 96
Methyl 2-cyclopentanonecarboxylate
Methyl 7-Azaindole-3-glyoxylate
Diethyl adipate, 99%
2,4-Difluoro-alpha,alpha-1-bis(1H-1,2,4-triazol-1-ylmethyl)benzylalcohol
Benzoin Ethyl Ether
Heptadecanoyl Chloride
BENZALDEHYDE AZINE
4-PENTYNOIC ACID
Dimethyl nitroterephthalate
Dichlorophenylborane
2,5-DiMethyl-3-furansulfonyl chloride
2,4-DiMethyldiphenylaMine
Ethyl 2-(5-broMo-2-Methylphenyl)-2,2-difluoroacetate
Triethylsilyl trifluoroMethanesulfonate, 99%
PotassiuM Sulfate, FCC
2-Carboxyphenyl Salicylate
1-Iodo-2-nitrobenzene
octahydro-1H-pyrrolo[3,4-b]pyridine
3-(trifluoromethyl)thiophenol
1-Bromo-4-(trifluoromethylthio)benzene
2,4-Dimethoxy-1-nitrobenzene
3-PHENYL-1-PROPYNE
CalciuM Acetylacetonate
5-Iodoindole, 98%
Methyl thiocyanate, 99%
L-Lysine dihydrochloride
3-AMino-3-phenyl-1-propanol
POTASSIUM (2-METHOXYPHENYL)TRIFLUOROBORATE
PotassiuM antiMonyl tartrate sesquihydrate
1-(4-Methylphenyl)-1-propanol
(1S,2R)-(+)-trans-2-(1-Methyl-1-phenylethyl)cyclohexanol
N-benzylguanidine(SALTDATA: 0.92CH3COOH 0.4H2O)
3-Methyl-1-phenyl-5-pyrazolone
3,4-(Methylenedioxy)phenylglyoxal hydrate
5-Chloro-2-thiophenecarboxamide
VANADIUM(III) CHLORIDE
Levosulpiride
1-NITROBUTANE
2,4-Dichlorotoluene
7-BroMo-5-fluoro-1-benzofuran
Ethyl 3-benzoylacrylate
2-(2-Nitrovinyl)thiophene
4-Methoxy-2-naphthylaMine
1-Methyl-1H-indole-6-carboxylic acid, 97%
SPERMINE DIHYDRATE
2,2-Bis(4-cyanatophenyl)propane
3-(2-thienyl)-L-alanine
2,3,4,6,7,8,9,10-octahydropyrimido[1,2-a]azepinium (p-tolylsulphonate)
1-Methyl-1,2,3-triazole
Sodium nitrite
5-TERT-BUTYL-2-METHOXYBENZENEBORONIC ACID
4-BroMo-1-trityl-1H-iMidazole
3-(Piperidin-1-ylMethyl)phenylboronic acid
N-Hydroxyurethane
5-Chloro-1H-indazole-3-carboxylic acid
(S)-Hexahydro-oxazolo[3,4-a]pyrazin-3-onehydrochloride
2-(p-Tolyl)pyridine ,97%
2-Amino-4'-methylbenzophenone, 99%
MEVINPHOS
YTTERBIUM NITRATE, PENTAHYDRATE
1,2-Dinitrobenzene
3,3-Dimethyl-1-butyne
(3-HydroxyMethyl-benzyl)-carbaMic acid tert-butyl ester
1,6-Bis(MaleiMido)hexane
3-(3'-Chlorobenzyloxy)phenylboronic acid
BenzenerutheniuM(II) chloride, diMer, 97%
3,4-Dihydro-2H-pyrido[3,2-b][1,4]oxazine, 97%
3',5'-Dibenzyloxyacetophenone
1,1,2,2,3,3,4-Heptafluorocyclopentane
4-chlorobutyric acid
3-Cyano-4-fluorobenzenesulphonyl chloride
7-Nitro-1-isoquinolinaMine
N-(5-chloro-2-methoxyphenyl)-3-hydroxy-4-[[2-methoxy-5-[(phenylamino)carbonyl]phenyl]azo]naphthalene-2-carboxamide
CK 18 ELISA Kit
CRYaB ELISA Kit
Human MUC17 ELISA Kit
Human Lung resistance-related protein,LRP ELISA Kit
Chicken BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
S100A11 ELISA Kit
Chicken IL-23 (Interleukin 23) ELISA Kit
Human delta sleep-inducing peptide,DSIP ELISA Kit
VEGF.C ELISA Kit
STAT2 ELISA Kit
NBB Agar Base,Modified
Yeast Synthetic Drop-out Agar Medium without Trypt
Butyl Isobutyrate
TRILINOLEIN
5-Chloro-8-Hydroxyquinoline
2-Fluorophenylacetonitrile, 97%
1-Iodo-2-nitrobenzene
Acid Red 315
rubidium iodide
3-Chloro-2-nitrobenzotrifluoride
4-Pentylcyclohexanone
ACIBENZOLAR-S-METHYL
3,5-Bis(trifluoromethyl)benzyl chloride
4-BroMo-3-(trifluoroMethyl)benzonitrile, 98%
4-Pentenoic acid, 98%
2,6-Dichloropyridine-3-boronic acid
CadMiuM acetate dihydrate
Methyl 4-hydroxypicolinate
2,5-DichlorobenzaMide
2-AMino-5-nitrobenzotrifluoride
N-Ethyl 5-broMopyridine-3-sulfonaMide
2-Methyl-1,3-oxazole-4-carboxylic acid, 97%
4-Hydroxyazobenzene-2'-carboxylic Acid
3-bromo-5-isopropyl-1H-1,2,4-triazole(SALTDATA: FREE)
3-Hydroxy-5-(trifluoromethoxy)benzeneboronic acid 98%
