EOMA细胞：小鼠血管内皮瘤传代细胞系 货号：GD-C4618764
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 4-Methoxybenzoate, 98+%
SodiuM Metasilicate
1-(4-CHLORO-PHENYL)-2-ISOPROPYLAMINO-ETHANOL
5-(4-FLUOROPHENYL)VALERIC ACID
1H-BenziMidazole-2-Methanol
1-(4-CHLOROPHENYL)-1-CYCLOHEXANECARBOXYLIC ACID
(R)-Boc-4-cyano-β-Phe-OH
2-oxo-2,3-dihydro-1,3-benzothiazole-6-sulfonyl chloride(SALTDATA: FREE)
Fmoc-L-homophenylalanine
N-Ethyl-beta-alaninenitrile, 98%
Methyl(diphenyl)phosphine Oxide
2-Chloro-4,6-dimethylpyrimidine
TETRAETHYLAMMONIUM P-TOLUENESULFONATE
Zinc bromide
Ethyl 6-bromohexanoate
Silver acetate
2,5-DiaMino-4,6-dihydroxypyriMidine Hydrochloride
2-BroMothiazole-5-Methanol
tert-Butyl carbaMate, 98%
Molybdic Acid, 85 Percent, Reagent, ACS
3-Ethyl-3-Methylglutaric Acid
2-methyl-1-propanethiol
1,4-Benzenediboronic acid
1-Chloro-1-phenylethane
Ethyl 2-phenylbutyrate
Chlorodiphenylmethylsilane
Methyl 3-mercaptopropionate
Urea Hydrogen Peroxide Adduct
5-Methoxy-2-(trifluoroMethyl)pyridine, 97%
2,4-DibroMobenzyl alcohol, 98%
4-Fluorostyrene
N-Benzyl-2-naphthylaMine
Diethyl sec-butylMalonate, 96%
StrontiuM oxalate, 95%
2-Ethoxy-4-fluorophenylboronic acid
2-Methyl-2-thiazoline
3-Amino-5-methylhexanoic acid, 98%
1-(1-Phenylethyl)
[3-(4-pyridinyl)-1,2,4-oxadiazol-5-yl]methanol(SALTDATA: FREE)
5-(Hydroxymethyl)furfural, 98%
Pentafluorobenzyl chloride
3-CYCLOPROPYL-3-OXO-PROPIONIC ACID ETHYL ESTER
PYRUVIC ACID LITHIUM SALT
Hoechst 33258;BisbenziMide H33258
3-(EthoxyMethylene)-2,4-pentanedione
Acetic Acid (1S,2R)-2-[N-Benzyl-N-(Mesitylenesulfonyl)aMino]-1-phenylpropyl Ester [Reagent for double aldol reaction]
2,3,4,5-Tetrahydro-benzo[c]azepin-1-one
4-[4-Chloro-3-(trifluoroMethyl)phenyl]-4-piperidinol, 97%
Ethyl 3-exo-aMinobicyclo[2.2.1]heptane-2-exo-carboxylate hydrochloride, 98%
SODIUM METAVANADATE HYDRATE
TriMethyl 1,2,4-Cyclohexanetricarboxylate (cis- and trans- Mixture)
Girard's Reagent T
N-Boc-(4-piperidin-4-yl)butyric acid
(silanetetrayltetraethane-2,1-diyl)tetrakis(trichlorosilane)
S-alpine-borane
5-(4-DIETHYLAMINOBENZYLIDENE)RHODANINE
8-CARBOXYNAPHTHALENE-1-CARBOXAMIDE
2-BroMo-3-trifluoroMethylpyridine
2′,4′-DiMethoxypropiophenone
4-(Morpholin-4-ylcarbonyl)benzeneboronic acid
4-Cyanophenyl 4-(3-Butenyloxy)benzoate
4-Bromo-DL-mandelic Acid
2-Chloro-1-methylpyridinium p-Toluenesulfonate
Azathioprine
2-Acetyl-6-bromopyridine
1-Bromo-6-chlorohexane
Paeonol
1-Methyl-1H-benziMidazole-2-sulfonic acid
1,10-Bis[4-(ethoxycarbonyl)phenoxy]decane
Ethyl 4'-Methoxybiphenyl-4-carboxylate
alpha-Naphthoflavone, 99.5%
2,2,6,6-TetraMethyl-3,6-dihydro-2H-pyran-4-boronic acid pinacol ester, 97%
2,3,4-Trifluorophenol
tert-Butyl 2,6-Dichloroisonicotinate
4-(phenylmethyl)-1,1'-biphenyl
2-Chloro-3,6-difluorobenzaldehyde
3,5-Difluoroanisole
1-(bromomethyl)-4-(propan-2-yl)benzene
Porcine platelet activating factor,PAF ELISA Kit
IL18BP ELISA Kit
Human neutrophil elastase,NE ELISA Kit
Human GAL ELISA Kit
TNF-β ELISA Kit
CXCR4 ELISA Kit
Monkey αFP (Alpha-Fetoprotein) ELISA Kit
Human cholecystokinin，CCK ELISA Kit
Human STAT6 ELISA Kit
ECP ELISA Kit
BAT Broth
Dubos Oleic Agar Base
Oleyl alcohol, 99+%
ChroMiuM silicon Monoxide, 99.9% (Metals basis)
Floxuridine
Methyl 2-methylbutyrate
2-methyl-1-propanethiol
trisilver orthophosphate
Barium stearate
1-hydroxyanthraquinone
3-(2-HYDROXYPHENYL)PROPIONIC ACID
2-Bromothioanisole
CalciuM hydroxide
SodiuM hexafluorophosphate, 98%
Ethyl trans-3-(1-pyrrolidinyl)acrylate, 98+%
(-)-(R)-N,N-DiMethyl-1-[(S)-1′,2-bis(diphenylphosphino
BariuM titanate
Ethyl N-benzoyl-L-argininate hydrochloride
5-Chloro-2-Methyl-4-thiocyanatoaniline
5-(3-Chlorophenyl)-1H-pyrazol-3-aMine
2-AMino-N-cyclohexyl-N-MethylbenzylaMine
4-Benzyloxy-2-formylphenylboronic acid
3-(Difluoromethoxy)benzaldehyde 98%
Calcium propionate, 98+%
Kinetin riboside ,98%
