U-138 MG细胞系：人脑星形胶质母细胞株 技术硬 货号：GD-C3920575
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
复苏细胞注意事项：1)收到细胞后当天换液，全部更换成客户自己的新鲜培养基，放进培养箱，第二天再消化；2)边观察边消化，根据细胞的形态，终止消化时间，采取以半分钟间隔吹打细胞边缘，如可吹打下来，即终止消化。客户摸索比较好的消化时间；3)随细胞有一份细胞操作说明书，请客户仔细查看；4)记得加1%双抗，降低被污染的概率。另外尽早冻存留种，以备后用；5)售后时间为一周，于细胞本身的质量问题，而因乙方自己不当操作(不规范操作，消化过度，不加双抗导致的污染等)导致的细胞问题不在售后范畴；6)收到细胞后，如细胞状态不佳，或培养中遇到问题，烦请当天尽快联系，以便处理。当天及时反馈细胞情况和细胞照片是售后跟踪处理重要的参考依据，请您务必重视；7)刚收到细胞时，胎牛血清可以用15%培养两三天，利于细胞尽快恢复状态，细胞状态稳定后，再调回10%即可(其中1,2条针对于贴壁细胞，悬浮细胞详情请见细胞操作说明书)
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
4-n-Nonylbiphenyl, 98%
4-DiethylaMinobenzaldehyde
3-Tolylboronic acid
Methyl 3-cyclopentenecarboxylate
2,3-DICYANOPYRAZINE
(R)-2-AMino-1,2,3,4-tetrahydronaphthalene-2-carboxylic acid
trans-N,N'-DiMethylcyclohexane-1,2-diaMine , 98%
1-(4-Ethoxyphenyl) 97%
6-Chloro-2-pyridinecarbaldehyde
Monoethyl Itaconate
2-Acetylbenzenecarbonitrile
2-METHYLTHIO-3,5-METHYLPYRAZINE
Piroctone olamine
Ethyl (S)-2-[(S)-4-methyl-2,5-dioxo-1,3-oxazolidin-3-yl]-4-phenylbutyrate
(S)-Cbz-Phenylalaninol
4-MethylMorpholine N-oxide Monohydrate
DiphenyliodoniuM-2-carboxylate Monohydrate
3-BroMopyridine-2,4-diol
Styrene oxide, 97+%
MeradiMate, USP
3-Hydrazinobenzoic Acid
Calcium carbonate
4,6-Dichloro-5-methylpyrimidine
hexanenitrile
Isobutyl Acetate
1,2-BIS(DIMETHYLPHOSPHINO)ETHANE
Rhodium triiodide
5,6,7,8-Tetrahydroquinoline
4-Cyano-N-FMoc-L-phenylalanine, 95%
HexaMethylene oxide, 98%
Carnosol
(S)-(+)-2-Methyl
2,5-DiaMinopyridine dihydrochloride
tert-Butyl 3-oxoazetidine-1-carboxylate
1-(3-METHYLPHENYL) DIHYDROCHLORIDE HYDRATE
FMoc-D-glutaMic acid gaMMa-tert-butyl ester
2,3-Difluorochlorobenzene
2-Iodo-6-methoxy-pyridine
Bis[(2-diphenylphosphino)ethyl]ammonium chloride, min. 97%
Diallylphenylphosphine, 95%
2,5-Diamino-4,6-dichloropyrimidine
Delorazepam
Moxonidine
MonoethanolaMine;EthylolaMine;2-AMinoethanol;β-AMinoethyl alcohol;ColaMine
Hydrochlorothiazide
L-Leucine benzyl ester p-toluenesulfonate salt
2-Phenyl-2-(2-piperidyl)acetaMide Hydrate
1,1-DichlorodiMethyl ether, 97%
2,4-Dichlorophenyl isothiocyanate, 97%
CITRATE
DL-2-Hydroxybutyric Acid (contains PolyMolecular esterification product)
3,4-Dichloro-5-nitropyridine
Eflornithine
4-Ethoxybenzonitrile
n-Amyl 2-furyl ketone
9-FLUORENOL
2-(DODECYLOXY)ETHANOL
3,5-DIMETHYLBENZENESULFONYL CHLORIDE
4H-PYRAN-4-ONE
Tetrapropylammonium hydroxide, 25% in water
2-Fluoro-4-(trifluoromethyl)benzoyl chloride, 97%
(S)-(-)-alpha-Methylbenzyl isocyanate, 98%
Recombinant Isocitrate Dehydrogenase
1H,1H,10H,10H-PERFLUORO-1,10-DECANEDIOL
3-Amino-4-methoxybenzamide
3-IODOPHENYLACETONITRILE
ETHYL NONAFLUOROBUTYL ETHER
2-Chloro-1,3-thiazole-5-carbonyl chloride
1-(3-BroMophenyl)piperidine
(1-Ethylpropyl)benzene
t-Butyl 4-iodopyrazole-1-carboxylate
2-BroMo-1-(2,3-dihydro-1,4-benzodioxin-6-yl)ethan-1-one, 95%
ALDICARB SULFOXIDE
Methyl 4-BroMo-3-hydroxybenzoate
n-Undecane
Sodium Chlorate
2-HYDROXYADENOSINE
2-amino-3-nitro phenol
NO ELISA Kit
IL-21 ELISA Kit
Human TNFβ ELISA Kit
TG ELISA Kit
IL9 ELISA Kit
CD3d ELISA Kit
Monkey IL-16 (Interleukin 16) ELISA Kit
Human Opacity-associated proteins,OPAs ELISA Kit
Human atrial natriuretic factor,ANF ELISA Kit
NTRK3 ELISA Kit
Gram Negative Enrichment Broth
Bromocresol Purple Agar
Triethyl Orthobenzoate
Sodium persulfate
5-Hexynenitrile, 98%
2-Methylpyrazine
Calcium carbonate
Zinc Phosphate
Hexyl benzoate
1-Naphthylamine-7-Sulfonic Acid
2,4-Dimethylthiazole
AMMoniuM Sulfide
N-Acetyl-^b-alanine, 97%
Benzenesulfonyl hydrazide, 98%
HEXARHODIUM HEXADECACARBONYL
3-AMino-4-Methoxyphenylboronic acid hcl
1,3-Dicyanobenzene
Methyl 1-Methyl-1H-iMidazole-5-carboxylate
(R)-(?)-(3,5-Dioxa-4-phosphacyclohepta[2,1-a:3,4-a′]dinaphthalen-4-yl)diMethylaMine
2-Bromo-1-[2-(trifluoromethyl)phenyl]-1-ethanone, 95%
2'-Methylacetophenone
2,7-Naphthalenedithiol
1-methyl-1H-1,2,3-triazol-4-amine(SALTDATA: HCl)
