NPA细胞系：人甲状腺乳头状癌细胞株 售后强 货号：GD-C3819465
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
【冻存细胞操作】1)选对数增生期细胞(证明无支原体污染)，在冻存前1d换液；2)按常规方法把培养细胞制备成悬液，计数，使细胞密度达5×10(7)/ml左右密度，离心，去上清；3)加入配制好的冻存液(培养液6.8ml，小牛血清2ml，DMSO 1ml，5.6%NaHCO3 0.1ml)，按与去上清相同的量一滴一滴加入离心管中，然后用吸管轻轻吹打令细胞重悬。冻存细胞时培养液中加入保护剂10％二甲基亚砜(DMSO) 或甘油，可使冰点降低，使细胞内水分在冻结前透出细胞外；4)分装于无菌冻存管中，每管加1.5m悬液；5)旋好冻存管并仔细检查，一定要盖紧，做好标记；6)冻存：在特殊的仪器或简易的液氮容器中，按-1℃/min的速度，在30～40min时间内，下降到液氮表面，再停30min后，直接投入液氮中。要适当掌握下降冷冻速度，过快能影响细胞内水分透出，太慢则促进冰晶形成。操作时应戴防护眼镜和手套，以免液氮冻伤。【复苏细胞操作】1)从罐中取出冻存管；2)迅速放入36℃～37℃水浴，不时摇动，使其急速融化，30～60s内完成；3)冻存管用70％酒精擦拭消毒后，打开盖子，用吸管将细胞悬液注入离心管中，再滴加10ml培养液；4)低速离心(500～1000r/min) 5min，去上清后再用培养液洗一次；5)用培养液适当稀释后，装入培养瓶37℃培养，次日更换一次培养液后，继续培养。以后仍按常规进行培养。冻存细胞数量要充分，密度应达到10(7)/ml，在融后稀释20倍时，仍能保持5×10(5)/ml数量。
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
接收细胞相关问题：如不能在收到细胞后及时操作细胞，T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞，可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞，并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
2-Methoxy-pyridin-4-ylaMine
L-GlutaMic acid 5-tert-butyl 1-Methyl ester hydrochloride
2-Chloro-3,4-diMethoxybenzyl alcohol, 97%
LithiuM hydroxide
2-CHLORO-6-METHYLTHIOPHENOL
1,6-HEXANEDIAMINE DIHYDROCHLORIDE
4-Aminopyrazolo[3,4-d]pyrimidine
6-Phenylhexanoic acid, 99%
2-Aminoethanethiolsulfuric acid, 99%
1,1-Diphenylhydrazine Hydrochloride
3-Methyl-1H-pyrazol-5-amine
5-ACETYLVALERIC ACID
NIOBIUM(V) CHLORIDE
2-Chloropropene
4-AMino-3-broMopyridine
5-Fluoroquinazolin-4-ol
1-(DiMethylaMino)-3-[2-(3-Methoxyphenethyl)phenoxy]-2-propanol Hydrochloride
3-AMino-2-fluorobenzoic acid
3-(Phenylthio)thiophene, 97%
1,3,5-TRIMETHYLBENZENE
Cholesterol Acetate
Diallyl maleate
4,6-dichloro-5-methylpyrimidin-2-amine
methyl sorbate
1-Acetylindole
2-Formylphenoxyacetic acid
3,4-Dihydroxybenzoic acid
4,5-Difluorophthalic Acid
Methallylcyclopentane, 99%
Propargyl benzoate, 98%
Benzotrifluoride
4-Chlororesorcinol
2-Chloro-6-ethylaMinopyrazine
NeodyMiuM trifluoride
1,1'-BIS(DICYCLOHEXYLPHOSPHINO)FERROCENE
2-AMino-7-broMofluorene
Terephthalaldehyde Bis(diethyl Acetal)
4-Iodobenzoic acid, 98%
3-Amino-2-bromo-6-methoxypyridine ,97%
1-Acetyl-3-indolecarboxaldehyde, 98%
Glycol sulfite
2,3,6-TRIFLUOROBENZYL ALCOHOL
o-Tolunitrile
2-Nitropropane;β-Nitropropane
2-Chlorobenzonitrile
1-Acetyl-2-iMidazolidinone
2-(4-TrifluoroMethylphenyl)pyrrolidine
4-BroMobutyryl chloride, 95%
1-(4-AMino-2-hydroxyphenyl)ethan-1-one, Tech.
L-Tyrosine hydrochloride
1-Acetyl-4-piperidone
3,7-dimethyloct-7-en-1-ol
N-Boc-D/L-Alaninol
3,5-Dinitrobenzyl alcohol
anti-3-oxotricyclo(2.2.1.0(2,6))heptane-7-carboxy
2,3,5,6-Tetrafluoroaniline
alpha,alpha-Diphenyl-4-piperidinoMethanol
MARGAROYL CHLORIDE
1-Benzyloxy-2-Methoxy-4-propenylbenzene
1H,1H,5H-Octafluoropentyl acrylate
2',4'-Dimethoxyacetophenone
2-Amino-5-chloro-3-nitropyridine
4-Formylcinnamic acid, predominantly trans, 98%
TRANS-2-UNDECENAL
3-ACETAMIDOCOUMARIN
2-Phenyltoluene
2,4,5-Trifluorophenylacetic acid
Poly(ethylene glycol Methyl ether)
1-Phenyl-1-pyridin-2-ylMethanaMine dihydrochloride
Guanidine SulfaMate
2-AMinoMethylpyrazine
3-BroMo-5-fluorobenzotrifluoride, 97%
2-Methyl-1-phenyl-2-propanol
N,N'-Diacetylchitobiose
1-chloro-1-fluoroethylene
N-alpha-t-BOC-L-glutamic-gamma-benzyl ester
1-Methylcyclopropene
Brompheniramine hydrogen maleate
Porcine Heat Shock Protein 70,HSP-70 ELISA Kit
Mouse FMS-like tyrosine kinase 3,Flt3 ELISA Kit
Human hnRNP A2/B1 ELISA Kit
Human D2R/DRD2 (Dopamine Receptor D2) ELISA Kit
Monkey Cys-C (Cystatin C) ELISA Kit
IL15 ELISA Kit
TGFβ1 ELISA Kit
Human PR3-ANCA ELISA Kit
PDGFD ELISA Kit
TPS ELISA Kit
Cysteine Heart Blood Agar with antibiotics
Cysteine Heart Blood Agar with antibiotics
LithiuM acetate dihydrate, 99%
trans-N-Boc-4-cyano-L-proline Methyl ester, 97%
4-n-Butyl-4'-cyanobiphenyl, 98%
2-PhenoxythioacetaMide, 97%
Diallyl maleate
Methyl acetimidate hydrochloride
6-Iodoquinoline
2-Naphthylacetic acid
1,4-Diiodobenzene
Cyclopropyl carbinol
Citronellyl Isobutyrate
6-tert-Butyl-1-indanone, 97%
Tri-n-butyltin acetate, 98%
4-AMino-N,N-diethylaniline Monohydrochloride
2′,6′-Dichloroacetophenone
Methylparaben
4-(DiethylaMino)benzaldehyde-1,1-diphenylhydrazone
(±)-cis-Cyclopentane-1,2-dicarboxylic acid
SUCCINAMIDE
2-Chloro-6-fluorobenzyl Cyanide
6-hydroxy-4-methyl-1-benzofuran-3(2H)-one(SALTDATA: FREE)
Inosine 5'-Triphosphate Trisodium Salt Hydrate
4-tert-Butyl-3-nitrobenzeneboronic acid 98%
