Mouse/Human/Rat CASP3 / Caspase 3 ELISA Kit (Cell-Based ELISA) - LS-F1854

Mouse/Human/Rat CASP3 / Caspase 3 ELISA Kit (Cell-Based ELISA) - LS-F1854
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LS-F1854-1
1 plate
$615 USD
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SpecificationsPublicationsReviewsImagesPopular CASP3 / Caspase 3 Elisa Kits
Rat CASP3 / Caspase 3 ELISA Kit (Sandwich ELISA) - LS-F11016
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.312-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Human CASP3 / Caspase 3 ELISA Kit (Sandwich ELISA) - LS-F11014
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.31-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Rat CASP3 / Caspase 3 ELISA Kit (Sandwich ELISA) - LS-F4138
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Tissue Homogenates

Mouse CASP3 / Caspase 3 ELISA Kit (Sandwich ELISA) - LS-F4139
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.625-40 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Tissue Homogenates

Human CASP3 / Caspase 3 ELISA Kit (Sandwich ELISA) - LS-F4137
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Tissue Homogenates

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Product DescriptionLS-F1854 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human CASP3 / Caspase 3 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.
About CASP3 / Caspase 3
CASP3 / Caspase 3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Uniprot: P42574 NCBI: NM_004346 NP_004337.2

Synonyms: CASP3 ElisaKit, Apopain ElisaKit, CASP-3 ElisaKit, Caspase-3 ElisaKit, CPP32B ElisaKit, CPP32 ElisaKit, Cysteine protease CPP32 ElisaKit, SCA-1 ElisaKit, SREBP cleavage activity 1 ElisaKit, Yama ElisaKit, Procaspase3 ElisaKit, Caspase 3 ElisaKit, CPP-32 ElisaKit, PARP cleavage protease ElisaKit, Protein Yama ElisaKit

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SpecificationsType
 
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
 
CASP3 / Caspase 3
Reactivity
 
Mouse, Human, Rat
Specificity
 
Caspase 3
Manual
 

Intended Sample Types
 
Adherent Cell Cultures
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• 96-Well Cell Culture Clear-Bottom Microplate
• 10x TBS
• Quenching Buffer
• Blocking Buffer
• 10x Wash Buffer
• 100x Anti-Caspase 3 Antibody (Rabbit Polyclonal)
• 100x Anti-GAPDH Antibody (Mouse Monoclonal)
• HRP-Conjugated Anti-Rabbit IgG Antibody
• HRP-Conjugated Anti-Mouse IgG Antibody
• Primary Antibody Diluent
• Ready-to-Use Substrate
• Stop Solution
• Crystal Violet Solution
• SDS Solution
• Adhesive Plate Seals

Protocol
 

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
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