C99人结肠癌腺癌细胞株
产品货号：GD-C0219178
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
【细胞培养中细菌、霉菌的污染情况总结】细菌：细菌在普通倒置显微镜下为黑色细沙状，根据感染细菌的不同，可有不同的外形，培养液一般会浑浊变黄，对细胞生长影响明显。仔细检查一下器皿的灭菌情况，是否在高压灭菌时放气时间足够，压力足够！尤其是和储存培养液接触的移液管等物品，连续两次污染的话有可能造成储存液污染，一定要注意！下次使用前检查一下培养液是否存在浑浊的现象！可在培养液中加相应的抗生素处理；霉菌：培养液是清亮的，倒置显微镜下无杂质，37度孵箱培养2-3天，仍清亮，但出现絮状杂质，镜下可见呈细丝状的团状漂浮物，可看到明显的菌丝，细胞仍可生长，但时间长之后，细胞的活力状态变差，用硫酸铜溶液擦拭CO2孵箱内，再把水盘里也加上饱和量的硫酸铜。或者在培养箱的托盘加入饱和的消毒磷酸氢二钠高盐液体,可以防止霉菌污染。CO2孵箱被霉菌污染后，可把所有细胞暂时转移，采用过氧乙酸擦洗孵箱(包括隔板，箱壁)。并把过氧乙酸放置在孵箱内一个小时，使其蒸汽弥漫。待过氧乙酸的气味消散后，再移入细胞。孵箱应定期清洁(2月左右)，尤其在多雨的季节。其它培养箱清洗方法是：用84液擦洗－清水擦洗－75%酒精擦洗－紫外灯照。预防霉菌污染，可在培养基里加3u/ml的两性霉素或制霉菌素或放线菌素D或双抗；但细胞一旦污染，很难挽救，制霉菌素或放线菌素D或双抗都于事无补，建议舍弃该污染细胞，将环境彻底消毒，如果所有细胞都污染，可能是系统污染，检查一下培养基和器材，如果只是个别污染，可能是操作问题，就要注意操作。
(2S,3S)-3-(Boc-aMino)-1,2-epoxy-4-phenylbutane, 98%
Azocyclotin
MagnesiuM hydroxide
2,3-Difluorobenzonitrile
ETHYL CHLOROFLUOROACETATE
ETHYL 4-CHLORO-5,7-DIMETHYLQUINOLINE-3-CARBOXYLATE
HENEICOSANOIC ACID
1-[2-Chloro-4-(4-chlorophenoxy)phenyl]ethan-1-one , 95%
5-Chloro-2-methoxybenzonitrile, 97%
4-Bromo-7-methylisatin
Sodium Abietate
3',4'-DIMETHYLACETANILIDE
Deslanoside
2-Bromoanthraquinone
Benzil
Tri-p-tolyl phosphate
Diethyl cis-4-Cyclohexene-1,2-dicarboxylate
2,6-DiMethylaniline Hydrochloride
Undecanoic acid, 99%
SodiuM Glucoheptonate
Methyl 4-AMino-3-Methylbenzoate
1,2-Propylene diacetate
3,4,6-Trichloropyridazine
Benzylhydrazine monohydrochloride
2-Fluoro-4-methylphenol
CYCLOPROPANEMETHYLAMINE HYDROCHLORIDE
ACETIC ACID
5-BroMo-1H-Indole-3-Carboxylic Acid
2-Thiopheneacetic acid, 98%
2-(Chloroethyl)benzene, 99%
4-Dodecylbenzenesulfonic acid
Saikosaponin C
N-CAPRIC ACID ISOAMYL ESTER
PERFLUORO-2,5,8-TRIMETHYL-3,6,9-TRIOXADODECANOYL FLUORIDE
7-(TRIFLUOROMETHYL)QUINOLINE
2-BroMothiazole-4-carboxylic acid
1-(4-methyl-2-thienyl)ethanone(SALTDATA: FREE)
3-(4-Methylpiperazin-1-yl)benzoic acid
(2,3-Dihydro-benzo[1,4]dioxin-6-yl)-acetic acid
2,4,6-Trimethylphenyl isothiocyanate
Bromocresol Purple
N-(2-Hydroxyethyl)acetamide
2-NONENOIC ACID
4-Methoxybenzoyl chloride
2,3-Dihydro-1-benzofuran-5-carbonyl chloride
Tauroursodeoxycholic Acid Dihydrate
Tetrakis(ethylthio)tetrathiafulvalene [Organic Electronic Material]
2-AMino-2-Methyl-1-propanol, 99%
4-(PhenoxyMethyl)benzoic acid, 97%
S-[N-(3-PHENYLPROPYL)(THIOCARBAMOYL)]-L-CYSTEINE
1-Butyl-4-MethylpyridiniuM Chloride
Esmolol
2-Methoxy-5-nitro-3-picoline
1-Methyl-1H-imidazole-4-carboxylic acid
Methyl 5-methylsalicylate
4-Pyridinecarboxaldehyde
Cholesteryl palMitate
Z-Lys(Z)-OSu
ChlorodiMethylphenylsilane
β-Galactosidase [ 400 units/mg]
ethyl (4-formyl-2-methoxyphenoxy)acetate(SALTDATA: FREE)
Cinnamoyl chloride, predominantly trans, 98%
3-Chloro-2-(4-methylpiperazin-1-yl)pyridine
Sclareol
TRIBROMONEOPENTYL METHACRYLATE
2-(2-(3-(2-(7-Chloro-2-quinolinyl)-ethenylphenyl)-3-hydroxypropyl)phenyl)-2-propanol
2-phenyl-2-iMidazoline
Victoria Blue B
1-Benzyl-3-(MethylaMino)pyrrolidine
2-(t-ButylsulfaMoyl)-5-Methylphenylboronic acid
AMberlite? IR120, Na forM, ion-exchange resin
2,4-Difluorobenzaldehyde, 98%
2-(TriMethylsilyl)ethyl 3-Nitro-1H-1,2,4-triazole-1-carboxylate
2-Phenoxypropionic Acid
(E)-o-Hydroxycinnamic acid
Methyl 4-butylacetamino-3-methylbenzoate
2-(3-Fluorophenyl)-2-methylpropanoic acid
Butanoic acid,2-amino-3-oxo-,ethyl ester
GSK-3 ELISA Kit
TIEG1 ELISA Kit
Pre-APO-A1 ELISA Kit
Human ARSF ELISA Kit
IL-2 ELISA Kit
α2M ELISA Kit
Chicken BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit
Human MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit
Human VASH1 ELISA Kit
GYPE ELISA Kit
Acid Broth
Nutrient Agar with TTC
FORMATE ION CHROMATOGRAPHY STANDARD
PotassiuM hydrogen fluoride, 98%
5alpha-Androstane-3b,17b-diol
2-Fluoro-5-iodobenzonitrile, 97%
1,2-Propylene diacetate
Ethanone, 1-(3-hydroxy-2-pyridinyl)-
4-PHENYLPYRAZOLE
3-fluorobenzyl chloride
3-TERT-BUTYL-1-METHYL-2-PYRAZOLIN-5-ONE
Titanium tetraisopropanolate
7-Fluorooxindole
SodiuM n-heptafluorobutyrate, 98%
Bis(tert-butylcyclopentadienyl)diMethylhafniuM(IV), 98+%
2-AMinopyridine
4-Methylpyridine-2-carboxaldehyde
2'',4'',6''-TRIFLUOROPROPIOPHENONE
N-Methoxy-N-MethylbenzaMide
3-AMino-L-tyrosine dihydrochloride Monohydrate
(S)-3,3-Diphenyl-1-Methylpyrrolidino[1,2-c]-1,3,2-oxazaborole
3-(Methoxycarbonyl)-5-nitrobenzeneboronic acid
4-(4-Propylphenyl)benzoic Acid
(S)-(-)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl, 99+%
Potassium niobate (99.999%-Nb) PURATREM
