FTC-133甲状腺癌细胞株
产品货号：GD-C319050
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
我常看到一个比较复杂的四步消化法，这个挺有意思，我也是次听说，应该是网友自己发展出来的方法，很有参考价值。但我估计这个方法可能对付少数非常怪异的细胞才需要这么复杂的程序。我目前养过的近300株细胞里面，还没遇到这么怪异的。比如Caco2其实是很好消化的细胞，不需要胰酶孵育即可，只是有点成片分布。不要以为成片分布是自己没养好，这个视细胞而定。如果和标准形态不一致，那可能是自己没消化好导致的，但如果消化方法正确，仍然成片分布，甚至完全吹打成单细胞悬液，贴壁后仍然成片分布，这是细胞的特性，是因为贴壁过程中重新聚集了。这个时候你拼了命要去让它均匀分布，你的细胞之后会对你越来越不好；比较难消化的细胞(润洗方法5min还不能消化)，就以colon cancer为例，比如HCT15, LS411和KM12，这样的细胞一般需要用少量胰酶孵育，但也不需要很多，一般100 mm dish，一次Z多加入500ul，就足够了，一般我加300ul。即使这样难消化的细胞，一般不超过5min，即可见细胞成片移动，就应该停止消化。一些正常细胞也会有难消化的时候，比如tsDC细胞，但也只要用少量胰酶孵育，3min左右即可看到成片沙状移动。
4-Chloro-3-nitrobenzoic acid, 99%
ZirconiuM(IV) butoxide solution
2-HYDROXY-4-(METHYLTHIO)BUTYRIC ACID
(FORMYLMETHYL)TRIPHENYLPHOSPHONIUM CHLORIDE
(3S)-N-[(1R)-1-Phenylethyl]-1-azabicyclo[2.2.2]octan-3-aMine Dihydrochloride
4-Chlorothieno[3,2-d]pyriMidine
1-(2-Naphthyl)ethanol, 97%
2,2-Bis[4-(4-aminophenoxy)phenyl]propane
1-Hexyl-3-phenyl-2-thiourea
(S)-(+)-4,12-Bis(diphenylphosphino)-[2.2]-paracyclophane, min. 97% (S)-PHANEPHOS
2-Isobutyl-3-methylpyrazine
4-CHLORO-2,5-DIFLUOROBENZOYL CHLORIDE
PHOSPHORUS PENTAFLUORIDE
4-BROMO-3-NITROPHENYLBORONIC ACID
YttriuM fluoride
β-D-Ribofuranose 1,2,3,5-Tetraacetate
2-(2,4-Difluorophenyl)pyridine
Ethyl 2-(2-broMophenyl)acetaMide
Trifluoroacetic acid, sodiuM salt, 97%
PralidoxiMe Chloride, USP
1-AMinononadecane
3,5-Dinitrosalicylic acid
2-Hydroxy-3-Cyanopyridine
1-ethynyl-4-nitrobenzene
1-fluorodecane
2-PHENYL-2-PROPANOL
SODIUM SULFATE DECAHYDRATE
Epsilon-Caprolactone MonoMer
(-)-1,1'-Bis[(2R,5R)-2,5-diethylphospholano]ferrocene(1,5-cyclooctadiene)rhodiuM(I) tetrafluoroborate, 97%
1-Benzyl-4-cyano-4-phenylpiperidine hydrochloride, 99%
Phytin
N,N'-Bis(2-hydroxyethyl)oxaMide
(-)-ANABASINE
POTASSIUM 4-BROMOPHENYLTRIFLUOROBORATE
2-AcetylaMinophenylboronic acid
5-(4-BroMophenyl)isoxazole-3-carboxylic acid hydrazide
1-(5-Bromopyridin-3-yl)-2-methylsulfonyl-ethanone
Muscimol.hydrobromide
1,4-dioxane-2-carboxylic acid(SALTDATA: FREE)
2-(4-Hydroxy-phenyl)-N-phenyl-acetamide
DEGN
N-Acetylanthranilic acid
Bromine
3-Acetylpyridine;Methyl 3-pyridyl ketone;3-Acetopyridine
1,3-Cyclopentanediol
Ethyl 3-(1-azetidinyl)propionate
4-(2-OctylaMino)diphenylaMine
2-MethylaMino-5-Methyl-3-nitropyridine
L-Tyrosine disodiuM salt, 98%
Sodium metaphosphate
Triethoxy-1H,1H,2H,2H-heptadecafluorodecylsilane
Titanium nitride
Bathophenanthrolinedisulfonic acid, disodium salt
5-Chloroindole
Lithium perchlorate
(R)-(-)-1-Aminoindan
4'-HYDROXY-2'-METHYLACETOPHENONE
4-HYDROXY-4'-NITROBIPHENYL
2-Indanol
2,3-Dicyanohydroquinone, 98%
3-(3-Fluoro-4-methoxybenzoyl)propionic acid
(R)-(-)-4-Benzyl-3-propionyl-2-oxazolidinone
2-Chloropyrimidine-5-boronic acid
2,4-Dibromopyridine
2,3,4-TRIFLUOROBENZENESULFONYL CHLORIDE
3-BROMO-5-(TRIFLUOROMETHYL)BENZENESULFONYL CHLORIDE
1,2-EPOXYTETRADECANE
2-BroMo-1-(2-chlorophenyl)ethan-1-one
1,8-Bis(2-pyridyl)-3,6-dithiaoctane
4-BroMo-2,3,5,6-tetrafluorophenylboronic acid
Acetovanillone, 98%
Propyl butyrate, 98+%
L-Azetidine-2-carboxylic Acid [Antagonist of L-Proline]
Butyl Methyl Sulfide
carbamyl-B-methylcholine chloride
2,6-Dimethoxypyridine-3-boronic acid
2-(1-Piperazinyl)nicotinamide
1,2,3-triacetyl-5-deoxy-β-D-Riboturanose
Porcine intercellular adhesion molecule 3,ICAM-3 ELISA Kit
αCTx ELISA Kit
Human Mucin-7,MUC7 ELISA Kit
Human PARC/CCL18 ELISA Kit
GSH ELISA Kit
S100A6 ELISA Kit
IL4 ELISA Kit
Human δSIP-α (Delta Sleep Inducing Peptide α) ELISA Kit
Human VEGFR1 ELISA Kit
SEMA7A ELISA Kit
Schaedler Broth
Yeast Synthetic Drop-out Agar Medium
Ethyl vanillin isobutyrate
CalciuM glycerophosphate hydrate, 97%
5-Chloro-6-hydroxynicotinic acid, 98%
2-Fluorophenylhydrazine hydrochloride
3,5-Dinitrosalicylic acid
Acid Yellow 119
Tin (IV) iodide
17A-hydroxypregnenolone
BOC-L-2-(5-BROMOTHIENYL)ALANINE
Potassium chlorate
AluMinuM BroMide
Bis(2-nitrophenyl) disulfide, 98%
2-HydroxyMethyl-1,4-benzodioxane, 98%
2,4-Dichlorothiazole-5-carboxaldehyde, 96%
N-Nitroso-Methyl ethylaMine
Methyl 5-DOXYL-stearate, free radical
Methyl nitroacetate
INDIUM CHLORIDE, HYDROUS
PENTYLTRIPHENYLPHOSPHONIUM BROMIDE
4-TriMethylsilyl-3-butyn-2-ol, 97%
2,4,6-Trimethylbenzylamine, 99%
8-Hydroxyquinaldine
3-Oxo-3-(1-Cbz-piperidin-4-yl)-propionic acidethyl ester
