TE10人食道癌肿瘤细胞株
产品货号：GD-C219235
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
常规的细胞实验(增殖，凋亡，迁移，分化之类的)，受消化影响不是很大，如果不用胰酶去孵育而使用润洗的方法消化的话(难消化细胞例外)。但少数实验，比如病毒包装，对细胞代数，对细胞状态要求极高。这个时候，胰酶消化，就是润洗，都会对细胞包装病毒的能力有影响，传代次数一多，细胞包装能力就会逐渐下降(当然也有其它因素影响包装效率)。这个时候都不用胰酶消化，用一些盐溶液(不是EDTA液)即可。这些盐溶液通过影响ECM相关酶的活性，来使得细胞脱离基质附着表面，但不切割任何蛋白；EDTA的作用。许多人不用胰酶，只用EDTA，或者用trypsin/EDTA联合作用。这里要明白，trypsin切割ECM的一些负责粘连和附着的蛋白，而EDTA通过螯合Ca离子，作用于Integrin的活性，所以EDTA的作用更加温和。有的人在trypsin里添加一些EDTA，或者对付特别难消化的细胞，添加多一些EDTA，就是这个道理。一般不要试图延长消化时间(如果10min还消化不彻底的话)，而应该想其它办法；PBS洗涤。消化之前用PBS洗涤，是常见的操作，因为Serum含有YZtrypsin的蛋白。但这里面也有学问可以讲，对于一些难消化细胞，那么可以配制不含Ca，Mg离子的PBS，因为这些离子也会YZtrypsin的活性。但对于绝大部分胰酶或者EDTA溶液润洗即可消化的细胞，不需要配制如此溶液。
2,6-Dichloro-4-nitroaniline, 95%
BetaMethasone 17-valerate
Methyl Malonyl chloride
Vinylcyclooctane
Solvent Green 5
HYDRIDOCARBONYLTRIS(TRIPHENYLPHOSPHINE)IRIDIUM (I)
AllyltriphenylphosphoniuM broMide
(1R,2R)-2-(Diphenylphosphino)-2,3-dihydro-1H-inden-1-amine, min. 97% (10wt% in THF)
(3'-Methoxy-biphenyl-4-yl)-acetic acid
1-Amino-1-phenylpropan-2-one hydrochloride
4-(n-Pentyloxy)benzeneboronic acid
(S)-6,7-Dimethoxy-1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid hydrochloride
1-(2,4-DICHLOROBENZYL)PIPERAZINE
MECARBAM
2-Hydroxy-4-MethylpyriMidine hydrochloride
2-Chloro-4-fluorobenzoic acid
1,3-DicyclohexyliMidazoliuM Tetrafluoroborate
1-Boc-2-piperidinecarbaldehyde
YttriuM(III) nitrate hexahydrate, 99.9%, (trace Metal basis)
Squalane, NF
2-Naphthaleneboronic Acid (contains varying aMounts of Anhydride)
reichstein'S substance S 21-acetate
4-Methylbenzamidine hydrochloride
columbianetin
3-Fluorobenzaldehyde
(S)-(-)-2-METHOXYPROPIONIC ACID
1H-Purin-6-amine sulfate
SodiuM phosphotungstate octadecahydrate
2,4-DibroMo-N-(4-fluorobenzyl)aniline, 97%
N-Acetyl-L-tyrosine ethyl ester Monohydrate, 99%
1,5-Pentanedithiol, 96%
Isopropenyl chloroforMate
HEXAFLUORO-2-(P-TOLYL)ISOPROPANOL
O-TOLUALDEHYDE 2,4-DINITROPHENYLHYDRAZONE
TRIARACHIDIN
Dipropylene glycol MonoMethyl ether
3-(2-FURYL)-2-PHENYLPROPENAL
5-Bromosalicylaldehyde ,99%
3,6-Dichloro-benzo[b]thiophene-2-carbonylchloride
2-Bromo-4-fluoro-5-nitrophenol
1-adamantyl hydrosulfide(SALTDATA: FREE)
tert-Butyl L-alaninate hydrochloride
4-(Trifluoromethoxy)benzoic acid
2,4,6-TRIMETHYL DIPHENYL SULFIDE
TERT-BUTYL METHYL MALONATE
Phenylurea
Dansyl Fluoride
5-BroMo-2-phenoxypyridine
SeleniuM, 99.5+%, powder, 200 Mesh
LeMon Oil, Italian Type, Coldpressed, FCC
1,3,5-Triethylbenzene
p-Fluorotoluene
benzyl 2,4-dihydroxyphenyl ketone
3-Methyl-2-butylamine
Tetraethylthiuram disulfide
Trimethylsilyl trifluoromethanesulfonate
4-(Azetidin-1-ylsulfonyl)phenylboronic acid
Indigo
5-Nitro-8-(pyrrolidin-1-yl)quinoline
1,3-Dioxan-2-one
5-tert-Butoxycarbonylamino-pyridine-3-carboxylic acid ,97%
N-Methylhexylamine
H-Orn(Z)-OH
1H,1H-PERFLUORO-1-HEPTANOL
Edifenphos
4-Vinyl-1-cyclohexene
3-BUTOXYBENZENEBORONIC ACID
3-Cyclopentylpropionic acid
1-BroMo-3-chloro-2-Methylpropane
5-Chloro-2-cyanophenylboronic acid
Benzothiazole, 97%
2-Octyn-1-ol, 98%
N-(2,4-Dinitrophenyl)-L-leucine
2,5-DiMethyl-3-hexyne-2,5-diol
Tetrafluorophthalic acid
5-Chloro-2-thiophenecarboxylic acid hydrazide
Milnacipran Hydrochloride
1-(4-Cyanophenyl)-piperazine
AFGF-1 ELISA Kit
S100A7 ELISA Kit
Human TPM3 ELISA Kit
Human UBN1 ELISA Kit
Chicken Interferon β,IFN-β/IFNB ELISA Kit
β2-GP ELISA Kit
CT ELISA Kit
Human cholecystokinin octapeptide，CCK-8 ELISA Kit
Human PC ELISA Kit
PTF1α ELISA Kit
BTB Medium
Casein peptone agar (G.A)
Vanillin acetate
CeriuM(III) fluoride, anhydrous, 99.9% (REO)
5,6,7-TriMethoxyflavone, 97%
2-Fluoro-4-iodobenzonitrile, 98%
reichstein'S substance S 21-acetate
Alpha-Cedrene Epoxide
4-Cyclopropylphenol
4-Hydroxyphenethyl alcohol
BIS(CYCLOPENTADIENYL)TUNGSTEN DIHYDRIDE
NICKEL(II) AMIDOSULFATE TETRAHYDRATE
2-AMino-3-benzyloxypyridine
(+)-1,2-Bis[(2S,5S)-2,5-diethylphospholano]benzene(1,5-cyclooctadiene)rhodiuM(I) tetrafluoroborate, 97%
3-Fluorophthalic anhydride, 98%
4-Methoxyacetophenone
TetraethylthiuraM disulfide
2-METHOXYFURAN
2,6-DIFLUORO-3-METHYLPHENYLACETIC ACID
4-(N-BUTOXYCARBONYL)PHENYL ISOCYANATE
3-Cyanophenylboronic acid, pinacol ester
(1S)-(-)-1-(Naphth-1-yl)ethylamine
6-Bromo-1H-indole-2,3-dione
2-(Chloromethyl)-5-methylthieno[2,3-d]pyrimidin-4(3H)-one ,97%
Poly(4-chlorostyrene)
