Human Apolipoprotein C-I ELISA Kit (Sandwich ELISA) - LS-F34455 Catalog Size Price LS-F34455-1 1 plate $865 USD Request Bulk Add to Wish List Add to CartPerformance Guaranteed SpecificationsPublicationsReviewsImagesPopular Apolipoprotein C-I Elisa Kits Human Apolipoprotein C-I ELISA Kit (Sandwich ELISA) - LS-F3320 Type: Quantitative Sandwich ELISA Format: 96-Well Microplate Reactivity: Human Range: 3.2-10000 pg/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Cell Culture Supernatants, Serum, Plasma Human Apolipoprotein C-I ELISA Kit (Sandwich ELISA) - LS-F5230 Type: Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Human Range: 4.69-300 ng/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Serum, Plasma Mouse Apolipoprotein C-I ELISA Kit (Sandwich ELISA) - LS-F5921 Type: Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Mouse Range: 6.25-400 ng/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Serum, Plasma Human Apolipoprotein C-I ELISA Kit (Sandwich ELISA) - LS-F10866 Type: Quantitative Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Human Range: 1.56-100 ng/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Serum, Plasma Rat Apolipoprotein C-I ELISA Kit (Competitive EIA) - LS-F27339 Type: Quantitative Competitive EIA Format: 96-Well Strip Plate Reactivity: Rat Range: 5-100 µg/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Serum, PlasmaProduct DescriptionLS-F34455 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Apolipoprotein C-I in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Apolipoprotein C-I as low as 5.625 nanograms per millilter. About Apolipoprotein C-I Apolipoprotein (apo) C-I gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A copy of the apo C-I gene, apo C-I', is located 4 kb downstream in the same orientation, on the same chromosome. However, apo C-I' gene has no detectable mRNA product in any tissue, suggesting that it may be a pseudogene. The apoC-I gene is 4653 bases long and the apo C-I' is 4387 base pairs, and each contains four exons and 3 introns.P02654 NM_001645 NP_001636.1
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
Adhesive Plate Sealers
Coated 96-well Strip Plate
Detection Antibody Diluent
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
HRP-Streptavidin Conjugate Diluent
Sample Diluent
TMB Substrate
Stop Solution
Wash Buffer (25x)
Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- APOC1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- APOC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the APOC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of APOC1 can be calculated. Restrictions
For research use only. Guarantee
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