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Vimentin波形蛋白单克隆抗体

产品信息
  • Vimentin波形蛋白单克隆抗体
    英文名称 Vimentin
    中文名称 波形蛋白单克隆抗体
    别    名 FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.  
    规格价格 50ul/860元 购买    100ul/1580元 购买        大包装/询价
    说 明 书 50ul  100ul  
    研究领域 肿瘤  细胞生物  发育生物学  信号转导  干细胞  
    抗体来源 Mouse
    克隆类型 Monoclonal
    克 隆 号 3B7
    交叉反应 Human, Mouse, Rat, 
    产品应用 WB=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 53kDa
    细胞定位 细胞浆 细胞外基质 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 Recombinant human Vimentin Protein: 
    亚    型 IgG
    纯化方法 affinity purified by Protein G
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

    Function:
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

    Subunit:
    Homopolymer assembled from elementary dimers. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B (By similarity).

    Subcellular Location:
    Cytoplasm.

    Tissue Specificity:
    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

    Post-translational modifications:
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.

    Similarity:
    Belongs to the intermediate filament family.

    SWISS:
    P08670

    Gene ID:
    7431

    Database links:

    Entrez Gene: 7431 Human

    Entrez Gene: 22352 Mouse

    Entrez Gene: 81818 Rat

    Omim: 193060 Human

    SwissProt: P08670 Human

    SwissProt: P20152 Mouse

    SwissProt: P31000 Rat

    Unigene: 455493 Human



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

    Vimentin—波形蛋白。是五种主要的中间丝之一，存在于各种正常和病理性间质来源的组织，如纤维母细胞、内皮细胞、淋巴细胞等正常细胞和肉瘤、淋巴瘤、黑色素瘤等肿瘤。波形蛋白是负责维持细胞骨架完整性的蛋白之一。
    产品图片
    Sample: 
    Hela(Human) Cell Lysate at 30 ug
    Hela KO Vimentin (Human) Cell Lysate at 30 ug
    Primary: Anti- Vimentin (bsm-33170M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 53 kD
    Observed band size: 58 kD


    Sample:
    Raji Cell Lysate at 40 ug
    U87MG Cell Lysate at 40 ug
    Primary: Anti- Vimentin (bsm-33170M)at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 53kD
    Observed band size: 58kD
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