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NSE神经元特异性烯醇化酶单克隆抗体

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  • NSE神经元特异性烯醇化酶单克隆抗体
    英文名称 NSE
    中文名称 神经元特异性烯醇化酶单克隆抗体
    别    名 Gamma-enolase; Gamma enolase; Neural enolase; Neuron specific enolase; neurone-specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENO2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Neuron specific enolase; Neuron specific gamma enolase; NSE; ENOG_HUMAN; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal.  γ 烯醇化酶
    规格价格 50ul/860元 购买    100ul/1580元 购买        大包装/询价
    说 明 书 50ul  100ul  
    研究领域 肿瘤  细胞生物  免疫学  神经生物学  
    抗体来源 Mouse
    克隆类型 Monoclonal
    克 隆 号 15E2
    交叉反应 Human, Mouse, Rat, 
    产品应用 WB=1:500-2000 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 48kDa
    细胞定位 细胞浆 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human NSE: 
    亚    型 IgG
    纯化方法 affinity purified by Protein G
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008].

    Function:
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

    Subunit:
    Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

    Subcellular Location:
    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

    Tissue Specificity:
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

    Similarity:
    Belongs to the enolase family.

    SWISS:
    P09104

    Gene ID:
    2026

    Database links:

    Entrez Gene: 2026 Human

    Entrez Gene: 13807 Mouse

    Entrez Gene: 24334 Rat

    Omim: 131360 Human

    SwissProt: P09104 Human

    SwissProt: P17183 Mouse

    SwissProt: P07323 Rat

    Unigene: 511915 Human



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

    神经元特异性烯醇化酶(NSE)存在于神经元细胞和神经内分泌组织中,起源于神经内分泌细胞的肿瘤可产生过量的NSE。NSE也是小细胞肺癌的检测指标,70%左右的小细胞肺癌患者血中NSE升高,而其他组织型肺癌NSE升高的患者仅为10%~20%。
    产品图片
    Sample: A431 Cell (Human) Lysate at 40 ug
    Primary: Anti- NSE (bsm-33072M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 50 kD


    Sample: U87MG Cell (Human) Lysate at 40 ug
    Primary: Anti- NSE (bsm-33072M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 50 kD
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