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14-3-3 zeta+delta抗体

产品信息
  • 14-3-3 zeta+delta抗体
    英文名称 14-3-3 zeta+delta
    中文名称 14-3-3 protein ζ/δ抗体
    别    名 14 3 3 protein delta; 14 3 3 protein zeta/delta; 14 3 3 protein/cytosolic phospholipase A2; 14-3-3 protein zeta/delta; 14-3-3zeta; 14-3-3 delta; 14-3-3 zeta; 14-3-3delta; 1433Z_HUMAN; KCIP 1; KCIP-1; KCIP1; MGC111427; MGC126532; MGC13815; Phospholipase A2; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein zeta polypeptide; YWHAZ.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 肿瘤  细胞生物  神经生物学  信号转导  细胞凋亡  转录调节因子  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复） 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 27kDa
    细胞定位 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta:51-150/245 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    Function:
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    Subunit:
    Interacts with CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with SAMSN1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11. Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2.

    Subcellular Location:
    Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.

    Post-translational modifications:
    The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.

    Similarity:
    Belongs to the 14-3-3 family.

    SWISS:
    P63104

    Gene ID:
    7534 

    Database links:

    Entrez Gene: 7529 Human

    Entrez Gene: 7534 Human

    Entrez Gene: 22631 Mouse

    Entrez Gene: 54401 Mouse

    Entrez Gene: 25578 Rat

    Entrez Gene: 56011 Rat

    Omim: 601288 Human

    Omim: 601289 Human

    SwissProt: P29358 Cow

    SwissProt: P31946 Human

    SwissProt: P63104 Human

    SwissProt: P35215 Mouse

    SwissProt: Q9CQV8 Mouse

    SwissProt: P35213 Rat

    SwissProt: P63102 Rat

    SwissProt: P29361 Sheep

    Unigene: 492407 Human

    Unigene: 643544 Human

    Unigene: 1292 Rat

    Unigene: 8653 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片
    Sample: 
    Cerebrum (Mouse) Lysate at 40 ug
    Primary: Anti-14-3-3 zeta+delta (bs-6790R) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 27 kD
    Observed band size: 27 kD


    Sample: 
    Cerebrum (Rat) Lysate at 40 ug
    Primary: Anti-14-3-3 zeta+delta (bs-6790R) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 27 kD
    Observed band size: 27 kD


    Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). 
    Primary Antibody (green line): Rabbit Anti-14-3-3 zeta+delta antibody (bs-6790R),Dilution: 0.2μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<, Dilution: 1μg /test. 


    Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) 
    Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (bs-6790R) ; Dilution: 1μg /10^6 cells; 
    Isotype Control Antibody (grenn line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test. 
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