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Frizzled 1/Wnt receptor Wnt信号受体蛋白/Frizzled homolog 1抗体

产品信息
  • Frizzled 1/Wnt receptor Wnt信号受体蛋白/Frizzled homolog 1抗体
    英文名称 Frizzled 1/Wnt receptor
    中文名称 Wnt信号受体蛋白/Frizzled homolog 1抗体
    别    名 Frizzled 1; Frizzled-1; Frizzled homolog 1 (Drosophila); Fz1; FZD1; FzE1; Wnt receptor; FZD1_HUMAN.  
    规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    研究领域 肿瘤  细胞生物  免疫学  细胞周期蛋白  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/test IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 63/71kDa
    细胞定位 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human Frizzled 1:501-600/647 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK3 kinase, nuclear accumulation of beta catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt mediated inactivation of GSK3 kinase. Both pathways seem to involve interactions with G proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt, Wnt5B, Wnt6, Wnt7A or Wnt7B.

    Function:
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt, Wnt5B, Wnt6, Wnt7A or Wnt7B.

    Subcellular Location:
    Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (By similarity).

    Tissue Specificity:
    Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney.

    Similarity:
    Belongs to the G-protein coupled receptor Fz/Smo family. 
    Contains 1 FZ (frizzled) domain. 

    SWISS:
    Q13467

    Gene ID:
    7855

    Database links:

    Entrez Gene: 7855 Human

    Entrez Gene: 14367 Mouse

    Entrez Gene: 317674 Rat

    Omim: 601723 Human

    SwissProt: Q13467 Human

    SwissProt: Q9EQD0 Mouse

    SwissProt: Q8CHL0 Rat

    Unigene: 17631 Human

    Unigene: 150813 Mouse

    Unigene: 470210 Mouse

    Unigene: 24792 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片
    Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Frizzled 1) Polyclonal Antibody, Unconjugated (bs-6148R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

    Images provided by the Independent Validation Program (badge number 029757) Formalin-fixed and paraffin embedded human ovarian cancer labeled with Rabbit Anti-Wnt receptor Polyclonal Antibody (bs-6148R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody. Diffuse, low-level staining was observed in positive and negative control tissues.

    Blank control: A549. 
    Primary Antibody (green line): Rabbit Anti-Frizzled 1 antibody (bs-6148R) 
    Dilution: 3μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 3μg /test. 
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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