Calponin 1 + 2 + 3钙结合蛋白Calponin抗体

Calponin 1 + 2 + 3钙结合蛋白Calponin抗体

英文名称	Calponin 1 + 2 + 3
中文名称	钙结合蛋白Calponin抗体
别    名	smooth muscle; Basic calponin; Calponin 1; Calponin 2; Calponin 3; Calponin1; Calponin2; Calponin3; Calponin-1; Calponin-2; Calponin-3; Calponin 1 basic smooth muscle; Calponin H1; Calponin H1 smooth muscle; Calponins basic; CNN 1; Cnn1; CNN2_HUMAN; Sm Calp; SMCC.
	Specific References  (1)     |     bs-4902R has been referenced in 1 publications. [IF=15.20] Lovren, Fina, et al. "MicroRNA-145 Targeted Therapy Reduces Atherosclerosis." Circulation 126.11 suppl 1 (2012): S81-S90.  IHC-F ;  Mouse.   PubMed:22965997
规格价格	100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	100ul  200ul
研究领域	心血管  细胞生物  信号转导  干细胞  结合蛋白  细胞骨架
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Cow, Horse,
产品应用	ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:50-200 IEM=1:20-200 IGS=1:20-200 GICA=1:20-200 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	34kDa
细胞定位	细胞浆 细胞膜 细胞外基质 分泌型蛋白
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human Calponin 1/2/3:101-200/309
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain. Function: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.  Tissue Specificity: Heart and smooth muscle.  Similarity: Belongs to the calponin family.  Contains 3 calponin-like repeats.  Contains 1 CH (calponin-homology) domain.  SWISS: P51911 Gene ID: 1265 Database links: Entrez Gene: 1265 Human Omim: 602373 Human SwissProt: Q99439 Human Unigene: 651512 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Calponin 1 + 2 + 3) Polyclonal Antibody, Unconjugated (bs-4902R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Positive control: H9C2(2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA  Primary Antibody: rabbit Anti-Calponin 1 + 2 + 3 (bs-4902R); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA