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Adipose Triglyceride Lipase脂肪甘油三酯脂酶抗体

产品信息
  • Adipose Triglyceride Lipase脂肪甘油三酯脂酶抗体
    英文名称 Adipose Triglyceride Lipase
    中文名称 脂肪甘油三酯脂酶抗体
    别    名 PEDF R; Adipose Triglyceride Lipase; TTS 2.2; PEDFR; PEDF-R; ATGL; ATGL DESNUTRIN; Calcium independent phospholipase A2; Desnutrin; EC 3.1.1.3; IPLA2 zeta; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA; Pigment epithelium derived factor; Pnpla2; Transport secretion protein 2; Transport secretion protein 2.2; Triglyceride hydrolase; TTS2; TTS2.2; ZETA.  
    规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    研究领域 免疫学  生长因子和激素  糖尿病  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复） 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 55kDa
    细胞定位 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human ATGL:401-504/504 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

    Subunit:
    Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity.

    Subcellular Location:
    Lipid droplet. Cell membrane.

    Tissue Specificity:
    Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).

    Similarity:
    Contains 1 patatin domain.

    SWISS:
    Q96AD5

    Gene ID:
    57104

    Database links:

    Entrez Gene: 57104 Human

    Entrez Gene: 66853 Mouse

    Entrez Gene: 361676 Rat

    Omim: 609059 Human

    SwissProt: Q96AD5 Human

    SwissProt: Q8BJ56 Mouse

    SwissProt: P0C548 Rat

    Unigene: 654697 Human

    Unigene: 29998 Mouse

    Unigene: 19196 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

    Pathway: Glycerolipid metabolism; triacylglycerol degradation.
    产品图片
    Sample: Heart (Mouse) Lysate at 40 ug
    Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD


    Sample: Ovary (Mouse) Lysate at 40 ug
    Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD


    Sample: Testis (Mouse) Lysate at 40 ug
    Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD


    Sample: Lung (Mouse) Lysate at 40 ug
    Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD


    Sample: Heart (Mouse) Lysate at 40 ug
    Primary: Anti- Adipose Triglyceride Lipase (bs-3831R) at 1/300 dilution
    Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD


    Blank control: A431. 
    Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (bs-3831R) 
    Dilution: 3μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody: Goat anti-rabbit IgG-AF647
    Dilution: 3μg /test. 
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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