Bovine APOE / Apolipoprotein E ELISA Kit (Competitive EIA) - LS-F10343

Bovine APOE / Apolipoprotein E ELISA Kit (Competitive EIA) - LS-F10343

Catalog Number: LS-F10343 Type: Competitive EIA (enzyme immunoassay) kit Target: APOE / Apolipoprotein E Reactivity: Bovine Specificity: This assay has high sensitivity and excellent specificity for detection of bovine APOE. No significant cross-reactivity or interference between bovine APOE and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between bovine APOE and all the analogs, therefore, cross reaction may still exist. Description: LS-F10343 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Bovine APOE / Apolipoprotein E in samples of Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of APOE / Apolipoprotein E as low as 7.81 nanograms per millilter.

Intended Sample Types: Plasma, Serum Sample Size: 50 - 100 µl

Format: 96-Well Microplate Detection: Colorimetric - 450nm (TMB) Measurement: Quantitative Detection Range: 31.25 - 2000 ng/ml Sensitivity: 7.81 ng/ml Precision: Intra-assay: CV%<8% Inter-assay: CV%<10%

Quality Assurance: Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Kit Components: Coated 96-well Strip Plate Standard (Lyophilized) HRP-conjugate (100x) HRP-conjugate Diluent Sample Diluent Wash Buffer (25x) TMB Substrate Stop Solution Adhesive Plate Sealers

Background: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOE. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APOE. The competitive inhibition reaction is launched between with pre-coated APOE and APOE in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOE in the samples. The color development is stopped and the intensity of the color is measured.

Storage: Short term: 4°C; Long term: see manual. Restrictions: For research use only.

About APOE / Apolipoprotein EUniprot: P02649 NCBI: NM_000041 NP_000032.1

Synonyms: APOE ElisaKit, AD2 ElisaKit, Apo-E ElisaKit, Apolipoprotein E3 ElisaKit, Apolipoprotein E ElisaKit, LDLCQ5 ElisaKit, LPG ElisaKit

Description: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2.
(More About APOE / Apolipoprotein E)
  

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