产品库
登录
首页 找仪器 社区 百科 新品 供应商 品牌 应用 资料 仪企号 展会 标准 求购 招中标

RUNX2核心结合因子α1/成骨特异性转录因子/Cbfα1抗体

产品信息
  • RUNX2核心结合因子α1/成骨特异性转录因子/Cbfα1抗体
    英文名称 RUNX2
    中文名称 核心结合因子α1/成骨特异性转录因子/Cbfα1抗体
    别    名 RUNX2_HUMAN; Runt-related Transcription Factor 2; CBF alpha 1; CBF-alpha-1; PEBP2-alpha A; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; OSF-2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit; AML3; CLCD.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 干细胞  转录调节因子  表观遗传学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复） 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 57(hu)/67(mo,ratkDa
    细胞定位 细胞核 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human RUNX2:201-300/521 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008].

    Function:
    Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. [SUBUNIT] Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors (By similarity). Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 (By similarity). The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.

    Subunit:
    Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.

    Subcellular Location:
    Nucleus.

    Tissue Specificity:
    Specifically expressed in osteoblasts. 

    Post-translational modifications:
    Phosphorylated; probably by MAP kinases (MAPK). Isoform 3 is phosphorylated on Ser340.

    DISEASE:
    Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.

    Similarity:
    Contains 1 Runt domain.

    SWISS:
    Q13950

    Gene ID:
    860

    Database links:

    Entrez Gene: 860 Human

    Entrez Gene: 12393 Mouse

    Entrez Gene: 100155806 Pig

    Entrez Gene: 367218 Rat

    Omim: 600211 Human

    SwissProt: Q13950 Human

    SwissProt: Q9XSB7 Horse

    SwissProt: Q08775 Mouse

    SwissProt: Q9Z2J9 Rat

    Unigene: 535845 Human

    Unigene: 391013 Mouse

    Unigene: 391017 Mouse

    Unigene: 214214 Rat

    Unigene: 83672 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片
    Sample: 
    Cerebrum (Mouse) Lysate at 40 ug
    Primary: Anti-RUNX2 (bs-20003R) at 1/2000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 57/67 kD
    Observed band size: 57 kD


    Sample: U937 Cell (Human) Lysate at 40 ug
    Primary: Anti-RUNX2 (bs-20003R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 57/67 kD
    Observed band size: 57 kD


    Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug
    Primary: Anti-RUNX2 (bs-20003R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 57/67 kD
    Observed band size: 57 kD


    Sample: U937 Cell (Human) Lysate at 40 ug
    Primary: Anti-RUNX2 (bs-20003R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 57/67 kD
    Observed band size: 57 kD


    Blank control (blue line): U251 (fixed with 2% paraformaldehyde (10 min)and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). 
    Primary Antibody (green line): Rabbit Anti-RUNX2 antibody (bs-20003R),Dilution: 1μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test. 
    • 信息声明：本产品供应信息由仪器网为您整合，供应商为（上海联迈生物工程有限公司），内容包括 （RUNX2核心结合因子α1/成骨特异性转录因子/Cbfα1抗体）的品牌、型号、技术参数、详细介绍等；如果您想了解更多关于 （RUNX2核心结合因子α1/成骨特异性转录因子/Cbfα1抗体）的信息，请直接联系供应商，给供应商留言！
    供应商产品推荐
      您可能感兴趣的产品