Rat ADORA2A/Adenosine A2A Receptor ELISA Kit (Sandwich ELISA) - LS-F34417

Rat ADORA2A/Adenosine A2A Receptor ELISA Kit (Sandwich ELISA) - LS-F34417

Catalog Number: LS-F34417 Type: Sandwich ELISA (enzyme-linked immunosorbent assay) kit Target: ADORA2A/Adenosine A2A Receptor Reactivity: Rat Description: LS-F34417 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat ADORA2A/Adenosine A2A Receptor in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of ADORA2A/Adenosine A2A Receptor as low as 0.094 nanograms per millilter.

Intended Sample Types: Plasma, Serum, Tissue Homogenates

Format: 96-Well Strip Plate Detection: Colorimetric - 450nm (TMB) Measurement: Quantitative Detection Range: 0.156 - 10 ng/ml Sensitivity: 0.094 Precision: Intra-Assay: CV<8% Inter-Assay: CV<10%

Quality Assurance: Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Kit Components: Adhesive Plate Sealers Coated 96-well Strip Plate Detection Antibody Diluent Biotinylated Detection Antibody (100x) HRP-Streptavidin Conjugate (100x) HRP-Streptavidin Conjugate Diluent Sample Diluent TMB Substrate Stop Solution Wash Buffer (25x) Standard (Lyophilized)

Background: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ADORA2a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ADORA2a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ADORA2a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ADORA2a can be calculated.

Storage: Short term: 4°C; Long term: see manual. Restrictions: For research use only.

About ADORA2A/Adenosine A2A ReceptorUniprot: P29274 NCBI: NM_000675 NP_000666.2

Synonyms: ADORA2A ElisaKit, Adenosine receptor A2a ElisaKit, ADORA2 ElisaKit, A2a receptor ElisaKit, Adenosine A2a receptor ElisaKit, Adenosine A2 receptor ElisaKit, Adenosine receptor subtype A2a ElisaKit, HA2aR ElisaKit, RDC8 ElisaKit

Description: ADORA2A/Adenosine A2A Receptor is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways.
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