PSTPIP2脯氨酸-丝氨酸-苏氨酸磷酸酶相互作用蛋白2抗体

PSTPIP2脯氨酸-丝氨酸-苏氨酸磷酸酶相互作用蛋白2抗体

英文名称	PSTPIP2
中文名称	脯氨酸-丝氨酸-苏氨酸磷酸酶相互作用蛋白2抗体
别    名	Macrophage actin associated tyrosine phosphorylated protein; Macrophage actin-associated tyrosine-phosphorylated protein; MAYP; PPIP2_HUMAN; Proline serine threonine phosphatase interacting protein 2; Proline-serine-threonine phosphatase-interacting protein 2.
规格价格	100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	100ul  200ul
研究领域	细胞生物  信号转导  结合蛋白  细胞骨架
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Pig, Horse, Rabbit,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	39kDa
细胞定位	细胞膜
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human PSTPIP2:131-230/334
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	Function: Binds to F-actin. May be involved in regulation of the actin cytoskeleton (By similarity). Subunit: Homodimer (PubMed:19584923). Homotrimer PubMed:17964261). Interacts (via coiled-coil domain) with CD2AP, PTPN12 and PTPN18. Interacts (via SH3 domain) with ABL1 and WAS. Interacts (via SH3 and coiled-coil domains) with MEFV (via B-box zinc finger); the interaction allows binding of MEFV to PYCARD and facilitates formation of PYCARD pyroptosomes. Interacts with CD2, DNM2 and FASLG. Subcellular Location: Cell Membrane; peripheral membrane protein and Cytoplasmic (by similarity). Tissue Specificity: Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta. Post-translational modifications: Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV (By similarity).  DISEASE: PAPA syndrome (PAPAS) [MIM:604416]: Characterized by autosomal dominant inheritance of early-onset, primarily affecting skin and joint tissues. Recurring inflammatory episodes lead to accumulation of sterile, pyogenic, neutrophil-rich material within the affected joints, ultimately resulting in significant destruction.  Similarity: Contains 1 F-BAR domain. Contains 1 SH3 domain. SWISS: Q9H939  Gene ID: 9050 Database links: Entrez Gene: 9050 Human Entrez Gene: 19201 Mouse Entrez Gene: 307248 Rat SwissProt: Q9H939 Human SwissProt: Q99M15 Mouse Unigene: 567384 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Paraformaldehyde-fixed, paraffin embedded (rat uterus tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSTPIP2) Polyclonal Antibody, Unconjugated (bs-19580R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.