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MAP1D蛋氨酸氨肽酶1D抗体

产品信息
  • MAP1D蛋氨酸氨肽酶1D抗体
    英文名称 MAP1D
    中文名称 蛋氨酸氨肽酶1D抗体
    别    名 AMP1D_HUMAN; CDS of metAP 3 within PCR fragment; M ethionine aminopeptidase 1D, mitochondrial precursor; MAP 1D; MAP1D; MetAP 1D; Metap1l; Methionine aminopeptidase 1D; Methionine aminopeptidase 1D, mitochondrial; Methionyl aminopeptidase type 1D (mitochondrial); Methionyl aminopeptidase type 1D, mitochondrial; mitochondrial; Peptidase M 1D.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 细胞生物  转录调节因子  表观遗传学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Dog, Cow, Horse, Rabbit, Sheep, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 35kDa
    细胞定位 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human MAP1D:251-335/335 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]

    Function:
    Removes the amino-terminal methionine from nascent proteins (By similarity). May play a role in colon tumorigenesis.

    Subcellular Location:
    Mitochondrion.

    Tissue Specificity:
    Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level).

    Similarity:
    Belongs to the peptidase M24A family.

    SWISS:
    Q6UB28

    Gene ID:
    254042

    Database links:

    Entrez Gene: 254042 Human

    Entrez Gene: 66559 Mouse

    Entrez Gene: 311748 Rat

    Omim: 610267 Human

    SwissProt: Q6UB28 Human

    SwissProt: Q9CPW9 Mouse

    Unigene: 298250 Human

    Unigene: 152796 Mouse

    Unigene: 24026 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片
    Sample: 
    SW480(Human) Cell Lysate at 30 ug
    Primary: Anti-MAP1D (bs-18659R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 35 kD
    Observed band size: 35 kD


    Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1D) Polyclonal Antibody, Unconjugated (bs-18659R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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