CAPRIN2胃癌多药耐药相关蛋白

CAPRIN2胃癌多药耐药相关蛋白

英文名称	CAPRIN2
中文名称	胃癌多药耐药相关蛋白
别    名	C1q domain-containing protein 1; C1QDC1; CAPR2_HUMAN; Caprin family member 2; Caprin-2; caprin2; Cytoplasmic activation/proliferation-associated protein 2; EEG 1; EEG1; Gastric cancer multidrug resistance-associated protein; KIAA1873; Protein EEG-1; RNA granule protein 140; RNG140.
规格价格	100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	100ul  200ul
研究领域	细胞生物  信号转导  干细胞
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Chicken, Pig, Horse, Sheep,
产品应用	ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	126kDa
细胞定位	细胞浆
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human CAPRIN2:101-200/1127
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: Caprin2 is 1127 amino acid highly conserved protein that is ubiquitously expressed with highest levels of expression in brain and spleen. Caprin2 stabilizes cytosolic β-catenin and enhances LEF-1 dependent reporter gene activity as well as the expression of Wnt target genes in mammalian cells. Caprin2 promotes LRP5/6 phosphorylation by GSK-3 and enhances the interaction between Axin and LRP5/6. It is suggested that Caprin2 functions as a proapoptotic inhibitor of the cell cycle. Nine isoforms of Caprin2 exist due to alternative splicing events. Function: May regulate the transport and translation of mRNAs, modulating for instance the expression of proteins involved in synaptic plasticity in neurons. Involved in regulation of growth as erythroblasts shift from a highly proliferative state towards their terminal phase of differentiation. May be involved in apoptosis. Subcellular Location: Cytoplasm; Mitochondrion. Colocalizes with aggregated mitochondria and Mitochondrion. Cytoplasm. Expressed throughout the cytoplasm. Tissue Specificity: Detected in all tissues tested with highest levels of expression in brain and spleen. Similarity: Belongs to the caprin family. Contains 1 C1q domain. SWISS: Q6IMN6 Gene ID: 65981 Database links: Entrez Gene: 65981 Human Omim: 610375 Human SwissProt: Q6IMN6 Human Unigene: 234355 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (bs-12423R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: Mouse spleen cells (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).  Primary Antibody:Rabbit Anti-CAPRIN2 antibody(bs-12423R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );  Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.