MAdCAM-1粘膜血管定居因子抗体

MAdCAM-1粘膜血管定居因子抗体

英文名称	MAdCAM-1
中文名称	粘膜血管定居因子抗体
别    名	Mucosal addressin cellular adhesion molecule-1; Addressin mucosal; hMAdCAM 1; MACAM1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1; MADCA_RAT.
规格价格	50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	50ul  100ul  200ul
研究领域	肿瘤  心血管
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	40kDa
细胞定位	细胞膜
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from rat MAdCAM-1:151-250/394
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] Function: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes (By similarity). Subunit: Homodimer (Probable). Subcellular Location: Membrane; Single-pass type I membrane protein.  Tissue Specificity: Detected in Peyer patches and mesenteric lymph nodes but not in spleen. Similarity: Contains 3 Ig-like (immunoglobulin-like) domains.  SWISS: O70540 Gene ID: 54266 Database links: Entrez Gene: 17123 Mouse SwissProt: Q61826 Mouse Unigene: 391556 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.  MAdCAM-1（粘膜血管定居因子或黏膜地址素细胞黏附分子）、CD44等均参与癌症和肿瘤细胞与血管内皮的相互作用，与癌症和肿瘤细胞一起穿过血管内皮而进入血管内，参与血行播散转移。
产品图片	Sample:  Lymph node (Mouse) Lysate at 30 ug Spleen (Mouse) Lysate at 30 ug Primary: Anti- MAdCAM-1 (bs-0642R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;  Incubation: Anti-MAdCAM-1 Polyclonal Antibody, Unconjugated(bs-0642R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining