CyTOF 2质谱流式细胞仪

The CyTOF® 2 system, the world’s most advanced mass cytometry platform, brings high-parameter capabilities to protein research, providing an unparalleled capacity to generate high-resolution phenotypic and functional profiles of cells from normal and diseased states.

The CyTOF 2 mass cytometer analyzes individual cells labeled with stable metal isotopes using state-of-the-art inductively coupled plasma time-of-flight technology. With more than 120 detection channels, the CyTOF 2 has the capability to simultaneously resolve multiple elemental probes per cell at high acquisition rates, thereby maximizing the per-cell information obtained from a single sample.

Mass cytometry employs elemental tags that are uncommon in biological systems. Cells stained with metal-conjugated probes in a single-cell suspension are introduced into the CyTOF 2 and undergo a multistep process within the instrument to detect these metal tags and record the identity and amount of each probe on each cell.

CyTOF 2
Specification

Channels	121
Mass Range	89–209 amu
Abundance Sensitivity	0.3% for 159Tb
Instrument Response	400,000 counts/pg 159Tb
Detection Limit	500 antibodies/cell
Dynamic Range	4.5 orders of magnitude
Calibration	Automatic
Operating System	Windows® 7 Pro 64-bit
Data Storage	720GB RAID (mirrored)
Sample Introduction	Dual loop, syringe
Peak Throughput	1,000 events/sec
Flow Rate	45 μL/min
Replicate Sample CV (normalized)	<3%
Width	97 cm (38 in)
Height	132 cm (52 in)
Depth	79 cm (31 in)
Weight	285 kg (628 lb)

Chiller
Specification​

Width	38 cm (15 in)
Height	64 cm (25 in)
Depth	67 cm (27 in)
Weight	81 kg (178 lb)

Workstation
Specification

Operating System	Windows 7 Pro 64-bit
CPU	Intel® Core™ i7 3930K
RAM	8 GB 2,400 MHz DDR3
Hard Drives	1.44 TB (6 x 240 GB SSD Corsair® Force Series™ GS SATA3) in RAID 10 configuration
Data Storage	720 GB RAID (mirrored)
Video	NVIDIA® NVS™ 310 x16 512 MB
Power Supply	650 W Antec® TruePower®
Serial Adapter Board	8 Port Native PCI Express RS232
Monitor	ViewSonic® 24 in LED
Keyboard/Mouse	Logitech® MK120 Wired
DVD/Blu-ray	LG® SATA
Motherboard	ASUS® P9X79 WS PRO
Enclosure	Antec Titan 650
Data File Size	imd    0.3 MB/sec/channel fcs     12.4 bytes/event/channel txt      2.4 bytes/event/channel
Width	20 cm (8 in)
Height	46 cm (18 in)
Depth	58 cm (23 in)
Weight	35 kg (77 lb)

Autosampler (optional)
Specification​

Sample Volume	50–900 μL
Sample Format	3 x 96-well plate, up to 2 mL deep well
Sample Resuspension	Probe pipet motion
Reagent Reservoirs	Carrier (DIW)       250 mL Wash Solution    250 mL Rinse (DIW)         250 mL
Peak Throughput	1,000 events/sec
Flow Rate	45 μL/min
Replicate Sample CV (normalized)	<3%
Width	39 cm (16 in)
Height	24 cm (10 in)
Depth	36 cm (14 in)
Weight	20 kg (44 lb)

Site Requirements​

Argon Gas	Source	Liquid or compressed gas
	Pressure	522 ±7 kPa (80 ±1 psi)
	Flow Rate	20 L/m
	Purity	≥99.996%
	Oxygen	<5 ppm
	Hydrogen	<1 ppm
	Nitrogen	<20 ppm
	Water	<4 ppm
Exhaust	Flow Rate	280 L/s (600 cfm)
	Heat Dissipation	3,000 W (10,090 BTU/hr)
Electrical	CyTOF 2	Two dedicated 30 A single-phase 220–240 V AC,50–60 Hz
	Power Consumption	2 x 4,500 VA
	Chiller	Powered through instrument
	Power Consumption	2,300 VA (via instrument)
	Workstation	6 A, 100–240 V AC, 50–60Hz
	Power Consumption	1,050 VA
	Monitor	6 A, 110–230 V AC, 50–60 Hz
	Power Consumption	100 VA
	Autosampler	4.2 A, 100–240 V AC, 50–60 Hz
	Power Consumption	100 VA