描述
Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types.
With Lipofectamine® RNAiMAX Transfection Reagent you will get:
• Superior transfection efficiency requiring lower RNAi concentrations, leading to more effective gene knockdown with minimal nonspecific effects
• Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
• Superior transfection efficiencies for miRNA antagonists and mimics
• Compatibility with a broad range of cell types, providing the most versatile approach to all of your gene silencing experiments
• A simple and rapid protocol for consistent and reproducible results
High knockdown in a wide range of cells
Lipofectamine® RNAiMAX Transfection Reagent transfects a wide range of cell types (see figure). For gene silencing, Lipofectamine® RNAiMAX Transfection Reagent's high-efficiency transfections lead to the high levels of gene knockdown needed to achieve convincing results.
Simple, high-throughput–ready transfections
Simply mix Lipofectamine® RNAiMAX Transfection Reagent with siRNA, add to your cells, incubate, and measure gene knockdown. The simplicity and speed combined with high transfection efficiency make Lipofectamine® RNAiMAX Transfection Reagent ideal for high-throughput siRNA transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
Find an optimized Lipofectamine® RNAiMAX transfection protocol for your cell line.
规格
| Cell Type: |
Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells |
| Sample Type (Specific): |
Synthetic siRNA |
| Transfection Technique: |
Lipid-Based Transfection |
| Serum Compatible: |
Yes |
| High Throughput Compatibility: |
High Throughput-Compatible |
| Product Line: |
Lipofectamine® |
| Product Size: |
1.5 mL |
| Shipping Condition: |
Approved for shipment at Room Temperature or on Wet Ice |
| Green Features: |
Sustainable packaging |
内容及储存
Contains one vial (1.5 ml) Lipofectamine® RNAiMAX Transfection Reagent. Store at 2–8°C. Do not freeze
其它规格：
| 品Pai |
货号 |
品名 |
规格 |
| Fermentas |
K1622 |
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26617 |
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| Invitrogen |
11668-027 |
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| Invitrogen |
11668-019 |
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| Invitrogen |
L3000-008 |
转染试剂lipo3000 LIPOFECTAMINE 3000 |
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| Invitrogen |
L3000-015 |
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| Invitrogen |
15596-026 |
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| Invitrogen |
15596-018 |
一步法总RNA提取试剂 TRIzol Reagent[Invitrogen 15596-018] |
200ml |
| Invitrogen |
C11875500BT |
国产培养基RPMI 1640，无HEPES、丙酮酸钠 |
500ml |
| Invitrogen |
C11995500BT |
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500ml |
| Invitrogen |
25200-056 |
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100ml |
| Invitrogen |
25200-072 |
0.25%胰蛋白酶溶液 Trypsin-EDTA (0.25%)[Invitrogen 25200-072] |
500ml |
| Invitrogen |
12800-017 |
DMEM高糖培养基粉末 含丙酮酸钠 [Invitrogen 12800-017] |
10*1L |
| Invitrogen |
12100-046 |
DMEM高糖培养基粉末[Invitrogen 12100-046] |
10*1L |
| Invitrogen |
31600-034 |
DEME低糖培养基[Invitrogen 31600-034] |
10*1L |
| Invitrogen |
31800-022 |
1640培养基[Invitrogen 31800-022 10*1L] |
10*1L |
| Invitrogen |
18064-071 |
18064-071 SuperScript II反转录酶 |
4*10000u |
| Invitrogen |
14175-095 |
HBSS[Invitrogen 14175-095] |
500ml |
| Invitrogen |
17504-044 |
B-27无血清添加剂 [Invitrogen 17504-044] |
10ml |
| Invitrogen |
17502-048 |
N-2 添加剂 [Invitrogen 17502-048] |
5ML |
| Invitrogen |
21103-049 |
Neurobasal神经细胞培养基{Invitrogen 21103-049} |
500ml |
| Invitrogen |
10888-022 |
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500ml |
| Invitrogen |
R25001 |
博莱霉素[Invitrogen R25001] |
1.25ml |
| Invitrogen |
13778-150 |
Lipofectamine RNAiMAX Transfection Reagent转染试剂 [Invitrogen 13778-150] |
1.5ml |
| Invitrogen |
15140-122 |
青链霉素双抗 (10,000 U/mL)[Invitrogen 15140-122] |
100ml |
| Invitrogen |
31985-070 |
Opti-MEM I减血清培养基 [Invitrogen 31985-070] |
500ml |
| Invitrogen |
25030-081 |
L-谷氨酰胺100倍浓缩液 [Invitrogen 25030-081] |
100ml |
| Invitrogen |
15338-100 |
15338-100 脂质体LTX，含Plus试剂 |
1ml |
| Invitrogen |
18064-014 |
18064-014 SuperScript II反转录酶 |
10000u |
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